FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001756858

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001756858
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17624684
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC10717956.081215413564294Illumina Single End PCR Primer 1 (100% over 50bp)
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC475160.26959915990550526No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCC369440.209615105723314Illumina Single End PCR Primer 1 (98% over 50bp)
CAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAA213280.12101209871337268No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)