FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001756871

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001756871
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17805019
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC8651414.858972630133111TruSeq Adapter, Index 5 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG6915243.8838711713815073TruSeq Adapter, Index 5 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATATCGTATGC238210.13378811895679527TruSeq Adapter, Index 5 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATATCGTATG213340.1198201473416007TruSeq Adapter, Index 5 (97% over 49bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)