FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001757003

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001757003
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17789772
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT2926481.6450351359196733TruSeq Adapter, Index 13 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTA1398310.7860190675855767TruSeq Adapter, Index 13 (97% over 40bp)
AGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTC249970.14051332417301357TruSeq Adapter, Index 13 (96% over 31bp)
AGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCT227000.12760141051835852TruSeq Adapter, Index 13 (96% over 33bp)
AAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTC218920.12305947484880639TruSeq Adapter, Index 13 (97% over 34bp)
CACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTG200680.11280639234724313TruSeq Adapter, Index 13 (96% over 29bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)