FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001757015

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001757015
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16779236
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG10409206.203619759564738TruSeq Adapter, Index 4 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC7840544.672763408298208TruSeq Adapter, Index 4 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATATCGTATG248360.14801627440009785TruSeq Adapter, Index 4 (97% over 49bp)
AAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTT176620.10526105002635401TruSeq Adapter, Index 4 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATATCGTATGC170290.10148853022867073TruSeq Adapter, Index 4 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)