FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001757107

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001757107
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16135999
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTA250616915.53153913804779TruSeq Adapter, Index 27 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTAT14495528.98334215315705TruSeq Adapter, Index 27 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATATCGTA211600.13113535765588483TruSeq Adapter, Index 27 (97% over 43bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)