FastQCFastQC Report
Tue 24 Jan 2023
EGAF00001757757

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001757757
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences346442707
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTT17226050.49722651543650476No Hit
GTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTC13000580.3752591622602695No Hit
CTCCGAAGTTTCCCTCAGGATAGCTGGCGCTCTCGCAGACCCGACGCACC8745850.2524472249894988No Hit
GGCGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCGACCGATCCC8584390.24778671412471093No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT8467050.24439971830609208No Hit
GTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGG8390650.24219444746458466No Hit
CTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAA8034700.23192002133847778No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT7942030.22924512017509438No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT7612190.2197243540185131No Hit
GGCGTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCC7291670.21047260781275443No Hit
GTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGT7112990.20531504506457976No Hit
CCCGAAAGATGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTC6920950.19977184856715718No Hit
GGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCT6326360.1826091261895145No Hit
CTCTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGA5920300.17088828485571209No Hit
GGGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCC5868760.1694005929817423No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG5851240.1688948816578783No Hit
GTGGGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGT5840620.1685883374649881No Hit
CCGAAAGATGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCT5786770.16703396789934447No Hit
GTCGCTTTTTGATCCTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGA5785890.16700856687394489No Hit
CTCTGATCGTTTTTTCACTGACCCGGTGAGGCGGGGGGGCGAGCCCCGAG5752010.16603062739606178No Hit
GCCCTTCGTCCTGGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCCCCCTC5637620.16272878274213462No Hit
GTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATT5559030.1604602979851442No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA5550790.1602224520200392No Hit
GCTCGCTTGATCTTGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGC5481790.1582307807103008No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT5452370.15738157824751092No Hit
CCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGGCA5419160.15642297818669337No Hit
GCTTGATCTTGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCA5378600.15525222183418627No Hit
ATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACGATCTCAACC5341110.15417008042256175No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG5324530.15369150201219273No Hit
CCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGG5276330.1523002185755349No Hit
CTGGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTCCGTAGCGGTCC5105540.14737039911190858No Hit
CCCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCAT4923720.14212220088674No Hit
GTGGCGTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGT4887660.14108133614138973No Hit
CTTGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCT4864470.1404119613925081No Hit
GGCCGAAGTGGAGAAGGCTTCCATGTGAACAGCAGTTGAACATGGGTCAG4829820.13941179601740036No Hit
GGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTT4794750.13839950742562465No Hit
GTGGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCG4730010.1365308001706614No Hit
CTCGCTTGATCTTGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCC4647710.13415522699977056No Hit
GGTTGATATAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAG4629350.1336252692425706No Hit
GTGGTAAACTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTCAAC4365270.1260026524385748No Hit
GTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGA4309010.12437871870109825No Hit
GTCCTGAGAGATGGGCGAGCGCCGTTCCGAAGGGACGGGCGATGGCCTCC4230240.12210503827982154No Hit
GTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTTGT4194510.12107369891899615No Hit
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGC4159120.12005217359071149No Hit
CGACTGTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGA4106840.11854312176356478No Hit
CTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCG4029080.11629859479189442No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA3971640.11464060058854117No Hit
CAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATA3931370.11347821502849531No Hit
CCCAGAAAAGGTGTTGGTTGATATAGACAGCAGGACGGTGGCCATGGAAG3863010.1115050171917748No Hit
CTTCGTCCTGGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCCCCCTCGCC3858230.11136704344017263No Hit
GGCCACTTTTGGTAAGCAGAACTGGCGCTGCGGGATGAACCGAACGCCGG3822760.11034320892776076No Hit
CCTCAGGATAGCTGGCGCTCTCGCAGACCCGACGCACCCCCGCCACGCAG3819680.11025430533886228No Hit
CTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCT3793000.1094841924324301No Hit
GGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTCCGTAGCGGTCCTGA3780970.10913694886929745No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA3735520.10782504363701326No Hit
CCTTCGTCCTGGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCCCCCTCGC3726290.10755862151833376No Hit
GCCGAAGTGGAGAAGGCTTCCATGTGAACAGCAGTTGAACATGGGTCAGT3703970.10691435914683578No Hit
GCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATG3690640.1065295913416356No Hit
CCCCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGG3682030.10628106539994217No Hit
GTGTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCA3680190.1062279541650158No Hit
GCCGAAACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCG3673220.10602676649793064No Hit
GTGCCTAGTGGGCCACTTTTGGTAAGCAGAACTGGCGCTGCGGGATGAAC3666640.10583683610346573No Hit
GGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA3665510.10580421887766855No Hit
GTCCGTAGCGGTCCTGACGTGCAAATCGGTCGTCCGACCTGGGTATAGGG3568630.10300779689958951No Hit
GTCAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCA3549550.10245705648524447No Hit
CTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGT3517970.10154550605101928No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)