FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001775039

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001775039
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11306
Sequences flagged as poor quality0
Sequence length40-635
%GC56

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCAGACGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGTCCTC167614.823987263399966No Hit
CAACGTCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTGTCCTC8447.465062798514063No Hit
ACCAGACGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTCGA4664.121705289226959No Hit
ACCAGACGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTCTG4423.909428621970635No Hit
ACCAGACGGGTGGGAACACCTTGTTCAGGTCCTCGAGCACTGTCAGCCGG4303.803290288342473No Hit
CAACGTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCCGG4083.608703343357509No Hit
CAACGTCGGGTGGGAACACGTTTTCAGGTCCTCTCCAGGCACTGTCCTCA3322.9364938970458168No Hit
ACCAGACGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAGAGGG3202.8303555634176543No Hit
ACCAGACGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAGAGGT2792.4677162568547675No Hit
CAACGTCGGGTGGGAACACGTTTTTCAGGTCCTCTACAACTGTGAGTCTG2472.184680700513002No Hit
ACCAGACGGGTGGGAACACCTTGTTCAGGTCCTCTAGCACGGTGAGCCGT2302.0343180612064393No Hit
ACCAGACGGGTGGGAACACCTTGTTCAGGTCCTCTGTGACCGTGAGCCTG2251.990093755528038No Hit
CAACGTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAGAGGT2201.9458694498496374No Hit
CAACGTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGGATGGAGAGTCGA2181.928179727578277No Hit
CAACGTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCCTG2071.8308862550857952No Hit
ACCAGACGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACCTG1991.7601273660003538No Hit
CAACGTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCCGT1931.7070581991862728No Hit
ACCAGACGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAGCCTG1451.2825048646736246No Hit
ACCAGACGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAGCTGG1421.255970281266584No Hit
ACCAGACGGGTGGGAACACCTTGTTCAGGTCCTCTGGTCAATATCTGATG1281.1321422253670617No Hit
CAACGTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAGAGGG1141.0083141694675395No Hit
CAACGTCGGGTGGGAACACGTTTTTCAGGTCCTCTACAACGGTTAACCTG1100.9729347249248187No Hit
ACCAGACGGGTGGGAACACCTTGTTCAGGTCCTCGAGCACCAGGAGCCGC1090.9640898637891385No Hit
ACCAGACGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGAGCCAA780.6898991685830532No Hit
CAACGTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTCACAGTGAGCCTG730.6456748629046524No Hit
CAACGTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCCGC710.627985140633292No Hit
ACCAGACGGGTGGGAACACCTTGTTCAGGTCCTCCAGTACGGTCAGCCTA670.5926056960905713No Hit
CAACGTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGTCAATATCTGATG650.574915973819211No Hit
CAACGTCGGGTGGGAACACGTTTTTCAGGTCCTCCAAGACAGAGAGCTGG620.5483813904121705No Hit
ACCAGACGGGTGGGAACACCTTGTTCAGGTCCTCTCTGCCCATCCCGCCC570.5041570847337697No Hit
CAACGTCGGGTGGGAACACGTTTTCAGGTCCTCGAGCACTGTCAGCCGGG500.44224305678400844No Hit
CAACGTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCCTA480.42455333451264815No Hit
CAACGTCGGGTGGGAACACGTTTTTCAGGTCCTCTACAACAGTGAGCCAA380.33610472315584644No Hit
CAACGTCGGGTGGGAACACGTTTTTCAGGTCCTCTCTGCCCATCCCGCCC380.33610472315584644No Hit
CAACGTCGGGTGGGAACACGTTTTCAGGTCCTCTACAACTGTGAGTCTGG360.3184150008844861No Hit
ACCAGACGGGTGGGAACACCTTGTTCAGGTCCTCCAGCACTGAGAGCCGG350.309570139748806No Hit
CAACGTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCCGG340.3007252786131258No Hit
ACCAGACGGGTGGGACACCTTGTTCAGGTCCTCTCCAGGCACTGTCCTCA340.3007252786131258No Hit
CAACGTCGGGTGGGAACACGTTTTCAGGTCCTCTAGCACGGTGAGCCGTG310.27419069520608524No Hit
CAACGTCGGGTGGGAACACGTTTTCAGGTCCTCTGTGACCGTGAGCCTGG310.27419069520608524No Hit
CAACGTCGGGTGGGAACACGTTTTCAGGTCCTCTAGGATGGAGAGTCGAG290.25650097293472496No Hit
ACCAGACGGGTGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGTCCTCA240.21227666725632408No Hit
CAACGTCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGTCCTC240.21227666725632408No Hit
ACCAGACGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTCCTAACT210.18574208384928356No Hit
CAACGTCGGGTGGGAACACGTTTTCAGGTCCTCGAGCACCAGGAGCCGCG200.1768972227136034No Hit
ACCAGACGGGTGGGAACACCTGTTCAGGTCCTCTCCAGGCACTGTCCTCA200.1768972227136034No Hit
ACCAGACGGGTGGGAACACCTTGTTCAGGTCCTAACTGCTGTGCCACTCTGAGCGGGCTGGCAAGGCGCATA180.15920750044224305No Hit
ACCAGACGGGTGGGAACACCTTGTTCAGGTCCTCTCCTAACTGCTGTGCC170.1503626393065629No Hit
CAACGTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAGCCTG160.1415177781708827No Hit
CAACGTCGGGTGGGAACACGTTTTCAGGTCCTCTGGAAAGGGAAGAGGTA160.1415177781708827No Hit
ACCAGACGGGTGGGAACACCTTGTCAGGTCCTCTCCAGGCACTGTCCTCA160.1415177781708827No Hit
ACCAGACGGGTGGGAACACTTGTTCAGGTCCTCTCCAGGCACTGTCCTCA130.1149831947638422No Hit
ACCAGACGGGTGGGAACACCTTGTTCAGGTCACCTAACTGCTGTGCCACTCTGAGCGGGCTGGCAAGGCGCATA130.1149831947638422No Hit
CAACGTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTCGA130.1149831947638422No Hit
ACCAGACGGGTGGGAACACCTTGTTAGGTCCTCTCCAGGCACTGTCCTCA120.10613833362816204No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)