FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001775040

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001775040
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences396806
Sequences flagged as poor quality0
Sequence length55-451
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTCACTCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAG354918.944169190990056No Hit
CGCTCACTCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAG230775.815688270842679No Hit
CTGACTGTAGCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAG223145.62340287193238No Hit
CTGACTGTAGCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAG214255.399363920908454No Hit
CTGACTGTAGCGGGTGGGAACACGTTTTTCAGGTCCTCTAGGATGGAGAG191314.821247662585748No Hit
CTGACTGTAGCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAG189884.7852099010599645No Hit
CTGACTGTAGCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTGT148953.7537234820038003No Hit
CGCTCACTCTCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGT145553.668039293760679No Hit
CGCTCACTCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAG136763.446520465920374No Hit
CTGACTGTAGCGGGTGGGAACACGTTTTTCAGGTCCTCTACAACTGTGAG126953.199296381607133No Hit
CTGACTGTAGCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAG126593.1902239381460964No Hit
CGCTCACTCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAG93622.359339324506182No Hit
CGCTCACTCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGCACGGTGAG89182.2474458551534No Hit
CGCTCACTCTCGGGTGGGAACACCTTGTTCAGGTCCTCGAGCACTGTCAG88582.2323251160516726No Hit
CGCTCACTCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAA81322.0493641729207726No Hit
CTGACTGTAGCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAG80662.032731359908872No Hit
CGCTCACTCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTGACCGTGAG71161.793319657464857No Hit
CTGACTGTAGCGGGTGGGAACACGTTTTTCAGGTCTTCTGATGGCTCAAA55181.3906039727221866No Hit
CGCTCACTCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGTCAATATCT54811.3812795169427883No Hit
CTGACTGTAGCGGGTGGGAACACGTTTTTCAGGTCCTCTGTCACAGTGAG50891.2824906881448364No Hit
CTGACTGTAGCGGGTGGGAACACGTTTTTCAGGTCCTCTACAACGGTTAA46901.18193777311835No Hit
CTGACTGTAGCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAG40381.0176257415462469No Hit
CGCTCACTCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAG34750.8757428063083723No Hit
CGCTCACTCTCGGGTGGGAACACCTTGTTCAGGTCCTCGAGCACCAGGAG32710.8243322933624996No Hit
CTGACTGTAGCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAG31520.794342827477407No Hit
CGCTCACTCTCGGGTGGGAACACCTTGTTCAGGTCAGGGAAGAAGCCTGT31010.7814901992409388No Hit
CGCTCACTCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGAG30160.7600691521801586No Hit
CTGACTGTAGCGGGTGGGAACACGTTTTTCAGGTCCTCTGGTCAATATCT29190.7356239572990327No Hit
CTGACTGTAGCGGGTGGGAACACGTTTTTCAGGTCCTCCAAGACAGAGAG19820.4994884149937249No Hit
CTGACTGTAGCGGGTGGGAACACGTTTTTCAGGTCAGGGAAGAAGCCTGT18470.4654667520148385No Hit
CGCTCACTCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTTAG18330.4619385795577688No Hit
CGCTCACTCTCGGGTGGGAACACCTTGTTCAGGTCTTCTGATGGCTCAAA17730.4468178404560415No Hit
CTGACTGTAGCGGGTGGGAACACGTTTTTCAGGTCCTCTACAACAGTGAG16490.41556831297913843No Hit
CGCTCACTCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAGTACGGTCAG15950.4019596477875838No Hit
CTGACTGTAGCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAG13870.3495410855682626No Hit
CGCTCACTCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAGCACTGAGAG13050.3288760754625686No Hit
CTGACTGTAGCGGGTGGGAACACGTTTTTCAGGTCCTCTCTGCCCATCCC11360.2862859936593701No Hit
CTGACTGTAGCGGGTGGGAACACGTTTTTCAGGTCCTCTACAACTGTTAG9560.24092377635418818No Hit
CTGACTGTAGCGGGTGGGAACACGTTTTTCAGGTTAGTATGCATCGACTC9320.2348754807134973No Hit
CTGACTGTAGCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTGTCCTCAGGAT7890.19883771918771392No Hit
CGCTCACTCTCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGTCCTCAGGAT7600.1915293619552124No Hit
CTGACTGTAGCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCCGTGTCCCTGGCCCGA7210.18170088153908961No Hit
CTGACTGTAGCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTGTCCTCAGGATGTTTTT6870.1731324627147775No Hit
CGCTCACTCTCGGGTGGGAACACCTTGTTCAGGTTAGTATGCATCGACTC6450.1625479453435684No Hit
CTGACTGTAGCGGGTGGGAACACGTTTTTCAGGTCCTCTGTTAGTATGCA5870.14793123087856536No Hit
CTGACTGTAGCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCCTGGTGCCCGGCCCGA5830.14692318160511686No Hit
CTGACTGTAGCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTTAG5670.1428909845113229No Hit
CTGACTGTAGCGGGTGGGAACACGTTTTTCAGGTCTTCTGATGGCTCAAACACAGCGACCTCGGGTGGGAA5460.13759872582571836No Hit
CTGACTGTAGCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCCGGGTGCCTGGGCCAAAAT5210.13129841786666532No Hit
CTGACTGTAGCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAG5010.1262581714994229No Hit
CGCTCACTCTCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGTCCTCAGGATGTTTTT4750.11970585122200773No Hit
CTGACTGTAGCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTTAG4740.11945383890364561No Hit
CGCTCACTCTCGGGTGGGAACACCTTGTTCAGGTCCTCTCTGCCCATCCC4440.11189346935278197No Hit
CGCTCACTCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTCGAGTCCCAT4340.10937334616916074No Hit
CGCTCACTCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTCTGGTGCCTTGT4210.10609718603045318No Hit
CTGACTGTAGCGGGTGGGAACACGTTTTTCAGGTCTTCTGATGGCTCAAATACAGCGACCTCGGGTGGGAA4170.10508913675700468No Hit
CGCTCACTCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAG4050.10206498893665923No Hit
CGCTCACTCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATTAGTAT3970.10004889038976225No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)