FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001775044

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001775044
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18792
Sequences flagged as poor quality0
Sequence length40-531
%GC55

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGCTCTCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAG7293.8793103448275863No Hit
TCGAGACTGTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGGATGGAGAG6823.6292039165602383No Hit
TCGAGACTGTCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTGT6143.2673478075776927No Hit
TGATCAGCTACGGGTGGGAACACGTTTTTCAGGTCCTCTACAACTGTGAG5983.1822051936994464No Hit
TCGAGACTGTCGGGTGGGAACACGTTTTTCAGGTCCTCTACAACTGTGAG5723.0438484461472965No Hit
TGATCAGCTACGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAG5653.006598552575564No Hit
TGATCAGCTACGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAG5402.8735632183908044No Hit
TCGAGACTGTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAG5302.820349084716901No Hit
TCGAGACTGTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAG5282.80970625798212No Hit
ATCGCTCTCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAG5022.67134951042997No Hit
ATCGCTCTCTCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGT4772.5383141762452106No Hit
TGATCAGCTACGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTGT4412.346743295019157No Hit
TGATCAGCTACGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAG4182.224350787569178No Hit
TGATCAGCTACGGGTGGGAACACGTTTTTCAGGTCCTCTAGGATGGAGAG4082.1711366538952745No Hit
CGACTCGAGACGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGT4022.1392081736909323No Hit
TCGAGACTGTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAG3531.8784589186888037No Hit
CGACTCGAGACGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAG3361.7879948914431671No Hit
ATCGCTCTCTCGGGTGGGAACACCTTGTTCAGGTCCTCGAGCACTGTCAG3331.7720306513409962No Hit
TGATCAGCTACGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAG3281.7454235845040442No Hit
ATCGCTCTCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGCACGGTGAG3071.6336739037888464No Hit
CGACTCGAGACGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAG2861.5219242230736483No Hit
TGATCAGCTACGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAG2781.4793529161345254No Hit
ATCGCTCTCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAG2581.3729246487867177No Hit
ATCGCTCTCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTGACCGTGAG2441.2984248616432525No Hit
ATCGCTCTCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAA2391.2718177948063005No Hit
TGATCAGCTACGGGTGGGAACACGTTTTTCAGGTCCTCTACAACGGTTAA2351.250532141336739No Hit
TCGAGACTGTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAG2101.1174968071519795No Hit
TCGAGACTGTCGGGTGGGAACACGTTTTTCAGGTCCTCTACAACGGTTAA1951.037675606641124No Hit
CGACTCGAGACGGGTGGGAACACCTTGTTCAGGTCCTCTAGCACGGTGAG1860.9897828863346105No Hit
CGACTCGAGACGGGTGGGAACACCTTGTTCAGGTCCTCGAGCACTGTCAG1840.9791400595998296No Hit
TCGAGACTGTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAG1790.9525329927628778No Hit
ATCGCTCTCTCGGGTGGGAACACCTTGTTCAGGTCCTCGAGCACCAGGAG1660.8833546189868029No Hit
TGATCAGCTACGGGTGGGAACACGTTTTCAGGTCCTCTCCAGGCACTGTC1660.8833546189868029No Hit
TCGAGACTGTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAG1360.7237122179650916No Hit
ATCGCTCTCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGTCAATATCT1340.7130693912303108No Hit
TGATCAGCTACGGGTGGGAACACGTTTTTCAGGTCCTCCAAGACAGAGAG1190.6332481907194552No Hit
ATCGCTCTCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAG1160.6172839506172839No Hit
CGACTCGAGACGGGTGGGAACACCTTGTTCAGGTCCTCTGTGACCGTGAG1150.6119625372498936No Hit
TGATCAGCTACGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAG1130.6013197105151128No Hit
ATCGCTCTCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAG1070.5693912303107705No Hit
TCGAGACTGTCGGGTGGGAACACGTTTTCAGGTCCTCTCCAGGCACTGTC990.5268199233716475No Hit
TGATCAGCTACGGGTGGGAACACGTTTTTCAGGTCCTCTGTCACAGTGAG990.5268199233716475No Hit
CGACTCGAGACGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAG900.4789272030651341No Hit
TGATCAGCTACGGGTGGGAACACGTTTTTCAGGTCCTCTGGTCAATATCT900.4789272030651341No Hit
ATCGCTCTCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGAG870.4629629629629629No Hit
TGATCAGCTACGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAG750.3991060025542784No Hit
TGATCAGCTACGGGTGGGAACACGTTTTCAGGTCCTCTACAACTGTGAGT750.3991060025542784No Hit
TGATCAGCTACGGGTGGGAACACGTTTTCAGGTCCTCGAGCACTGTCAGC720.38314176245210724No Hit
TGATCAGCTACGGGTGGGAACACGTTTTCAGGTCCTCTAGCACGGTGAGC650.34589186888037465No Hit
ATCGCTCTCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAGTACGGTCAG640.34057045551298426No Hit
TGATCAGCTACGGGTGGGAACACGTTTTTCAGGTCCTCTACAACAGTGAG640.34057045551298426No Hit
CGACTCGAGACGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAA630.33524904214559387No Hit
TGATCAGCTACGGGTGGGAACACGTTTTCAGGTCCTCTGGAAAGGGAAGA620.32992762877820353No Hit
TGATCAGCTACGGGTGGGAACACGTTTTCAGGTCCTCGAGCACCAGGAGC520.2767134951042997No Hit
TGATCAGCTACGGGTGGGAACACGTTTTCAGGTCCTCTGTGACCGTGAGC510.2713920817369093No Hit
CGACTCGAGACGGGTGGGAACACCTTGTTCAGGTCCTCGAGCACCAGGAG510.2713920817369093No Hit
TCGAGACTGTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTCACAGTGAG480.2554278416347382No Hit
ATCGCTCTCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAGCACTGAGAG450.23946360153256704No Hit
TGATCAGCTACGGGTGGGAACACGTTTCAGGTCCTCTCCAGGCACTGTCC450.23946360153256704No Hit
TCGAGACTGTCGGGTGGGAACACGTTTTCAGGTCCTCTAGCACGGTGAGC350.18624946785866325No Hit
TCGAGACTGTCGGGTGGGAACACGTTTTCAGGTCCTCGAGCACTGTCAGC350.18624946785866325No Hit
TCGAGACTGTCGGGTGGGAACACGTTTTCAGGTCCTCTAGGATGGAGAGT340.1809280544912729No Hit
TCGAGACTGTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAG340.1809280544912729No Hit
CGACTCGAGACGGGTGGGAACACCTTGTTCAGGTCCTCCAGTACGGTCAG330.1756066411238825No Hit
TCGAGACTGTCGGGTGGGAACACGTTTTTCAGGTCCTCCAAGACAGAGAG320.17028522775649213No Hit
TGATCAGCTACGGGTGGGAACACGTTTTCAGGTCCTCTAGGATGGAGAGT310.16496381438910176No Hit
TCGAGACTGTCGGGTGGGAACACGTTTTTCAGGTCCTCTCCCTGTGGCGG270.14367816091954022No Hit
TCGAGACTGTCGGGTGGGAACACGTTTTTCAGGTCCTCTACAACAGTGAG260.13835674755214986No Hit
TGATCAGCTACGGGTGGGAACACGTTTTTTCAGGTCCTCTAGCACGGTGA250.1330353341847595No Hit
TGATCAGCTACGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAG250.1330353341847595No Hit
TCGAGACTGTCGGGTGGGAACACGTTTTCAGGTCCTCTGTGACCGTGAGC250.1330353341847595No Hit
TCGAGACTGTCGGGTGGGAACACGTTTTTTCAGGTCCTCGAGCACTGTCA230.1223925074499787No Hit
CGACTCGAGACGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAG230.1223925074499787No Hit
TCGAGACTGTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAG220.11707109408258833No Hit
TGATCAGCTACGGGTGGGAACACGTTTTCAGGTCCTCCAAGACAGAGAGC210.11174968071519795No Hit
TGATCAGCTACGGGTGGGAACACGTTTTTTCAGGTCCTCTAGGATGGAGA210.11174968071519795No Hit
TCGAGACTGTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAG210.11174968071519795No Hit
TCGAGACTGTCGGGTGGGAACACGTTTTCAGGTCCTCCAGTACGGTCAGC210.11174968071519795No Hit
TGATCAGCTACGGGTGGGAACACGTTTTCAGGTCCTCCAGCACTGAGAGC200.10642826734780758No Hit
TCGAGACTGTCGGGTGGGAACACGTTTTCAGGTCCTCTGGAAAGGGAAGA190.1011068539804172No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)