FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001775045

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001775045
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9373
Sequences flagged as poor quality0
Sequence length40-576
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTAGTATCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAG111911.9385468900032No Hit
ATACTAGTATCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAG8268.812546676624347No Hit
ATACTAGTATCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAG5625.995945801771044No Hit
ATACTAGTATCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAG5385.739891176784381No Hit
ATACTAGTATCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAG3894.1502187133255095No Hit
ATACTAGTATCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTGT2562.731249333191081No Hit
ATACTAGTATCGGGTGGGAACACGTTTTTCAGGTCCTCTACAACTGTGAG2382.539208364451083No Hit
CGTCTGTCAGCGGGTGGGAACACGTTTTTCAGGTCCTCTGGTCAATATCT2212.3578363384188625No Hit
ATACTAGTATCGGGTGGGAACACGTTTTCAGGTCCTCTCCAGGCACTGTC1972.101781713432199No Hit
ATACTAGTATCGGGTGGGAACACGTTTTCAGGTCCTCTGTGACCGTGAGC1942.0697748853088656No Hit
ATACTAGTATCGGGTGGGAACACGTTTTTCAGGTCCTCTGGTCAATATCT1932.059105942601088No Hit
ATCGCGAGCACGGGTGGGAACACCTTGTTCAGGTCCTCTGTGACCGTGAG1831.9524165155233117No Hit
ATCGCGAGCACGGGTGGGAACACCTTGTTCAGGTCCTCGAGCACTGTCAG1821.9417475728155338No Hit
ATACTAGTATCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAG1821.9417475728155338No Hit
ATCGCGAGCACGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAG1791.909740744692201No Hit
TGCGTCGAGTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGTCAATATCT1661.7710444894910915No Hit
ATACTAGTATCGGGTGGGAACACGTTTTTTCAGGTCCTCTGTGACCGTGA1371.4616451509655393No Hit
ATCGCGAGCACGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGT1331.4189693801344287No Hit
ATACTAGTATCGGGTGGGAACACGTTTTTCAGGTCCTCTAGGATGGAGAG1131.2055905259788755No Hit
ATCGCGAGCACGGGTGGGAACACCTTGTTCAGGTCCTCTAGCACGGTGAG1121.194921583271098No Hit
ATACTAGTATCGGGTGGGAACACGTTTTCAGGTCCTCGAGCACTGTCAGC1041.1095700416088765No Hit
ATACTAGTATCGGGTGGGAACACGTTTTTTCAGGTCCTCTGGAAAGGGAA1031.098901098901099No Hit
ATACTAGTATCGGGTGGGAACACGTTTTCAGGTCCTCCAGTACGGTCAGC991.0562253280699883No Hit
ATACTAGTATCGGGTGGGAACACGTTTTTTCAGGTCCTCTAGCACGGTGA951.0135495572388777No Hit
ATCGCGAGCACGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAG951.0135495572388777No Hit
ATCGCGAGCACGGGTGGGAACACCTTGTTCAGGTCCTCCAGTACGGTCAG850.906860130161101No Hit
ATCGCGAGCACGGGTGGGAACACCTTGTTCAGGTCCTCTGGTCAATATCT790.842846473914435No Hit
ATACTAGTATCGGGTGGGAACACGTTTTTTCAGGTCCTCGAGCACTGTCA770.8215085884988798No Hit
TGCGTCGAGTCGGGTGGGAACACCTTGTTCAGGTCTCCCGGGTCCTAACT620.6614744478822149No Hit
ATACTAGTATCGGGTGGGAACACGTTTTCAGGTCCTCTGGAAAGGGAAGA600.6401365624666595No Hit
ATACTAGTATCGGGTGGGAACACGTTTTTCAGGTCCTCTACAACGGTTAA580.6187986770511043No Hit
ATACTAGTATCGGGTGGGAACACGTTTTCAGGTCCTCTAGCACGGTGAGC570.6081297343433265No Hit
ATCGCGAGCACGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAG530.565453963512216No Hit
CGTCTGTCAGCGGGTGGGAACACGTTTTTCAGGTCCTCTGGCCAATATCT520.5547850208044383No Hit
ATACTAGTATCGGGTGGGAACACGTTTTTTCAGGTCCTCTAGGATGGAGA520.5547850208044383No Hit
CGTCTGTCAGCGGGTGGGAACACGTTTTTCAGGTCTCCCGGGTCCCTAAC510.5441160780966606No Hit
CGTCTGTCAGCGGGTGGGAACACGTTTTTCAGGTCTCCCGGGTCCTAACT480.5121092499733276No Hit
ATACTAGTATCGGGTGGGAACACGTTTTCAGGTCCTCGAGCACCAGGAGC430.4587645364344394No Hit
TGCGTCGAGTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGCCAATATCT410.437426651018884No Hit
ATCGCGAGCACGGGTGGGAACACCTTGTTCAGGTCCTCGAGCACCAGGAG390.4160887656033287No Hit
ATACTAGTATCGGGTGGGAACACGTTTTTCAGGTCCTCCATTCCCCAAAC350.37341299477221807No Hit
TGCGTCGAGTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAG340.36274405206444044No Hit
ATACTAGTATCGGGTGGGAACACGTTTTTTCAGGTCCTCTACAACTGTGA310.33073722394110744No Hit
TGCGTCGAGTCGGGTGGGAACACCTTGTTCAGGTCTCCCGGGTCCCTAAC290.3093993385255521No Hit
TGCGTCGAGTCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGT280.2987303958177745No Hit
ATACTAGTATCGGGTGGGAACACGTTTTTTCAGGTCCTCCAGTACGGTCA270.2880614531099968No Hit
ATACTAGTATCGGGTGGGAACACGTTTTCAGGTCCTCTACAACTGTGAGT220.23471673957110847No Hit
ATCGCGAGCACGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAA200.2133788541555532No Hit
ATACTAGTATCGGGTGGGAACACGTTTTTTCAGGTCCTCTGGTCAATATC190.2027099114477755No Hit
TGCGTCGAGTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAG180.19204096873999787No Hit
ATACTAGTATCGGGTGGGAACACGTTTTTTCAGGTCCTCTCCAGGCACTG170.18137202603222022No Hit
ATACTAGTATCGGGTGGGAACACGTTTTCAGGTCCTCCATTCCCCAAACC150.16003414061666488No Hit
CGTCTGTCAGCGGGTGGGAACACGTTTTTCAGGTCCTCTGGTCAGTATCT140.14936519790888725No Hit
ATACTAGTATCGGGTGGGAACACGTTTTCAGGTCCTCTGGTCAATATCTG140.14936519790888725No Hit
TGCGTCGAGTCGGGTGGGAACACCTTGTTCAGGTCTCCCGGGCCCTAACT130.13869625520110956No Hit
ATACTAGTATCGGGTGGGAACACGTTTTTTCAGGTCCTCGAGCACCAGGA130.13869625520110956No Hit
ATACTAGTATCGGGTGGGAACACGTTTTCAGGTAAGCATAACCCTTACTT120.1280273124933319No Hit
ATACTAGTATCGGGTGGAACACGTTTCAGGTCCTCTCCAGGCACTGTCCT120.1280273124933319No Hit
ATACTAGTATCGGGTGGGAACACGTTTTCAGGTCCTCTACAACGGTTAAC120.1280273124933319No Hit
ATCGCGAGCACGGGTGGGAACACCTTGTTCAGGTAAGCATAACCCTTACT110.11735836978555424No Hit
TGCGTCGAGTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTGACCGTGAG100.1066894270777766No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)