FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001775047

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001775047
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14644
Sequences flagged as poor quality0
Sequence length40-523
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGCTGTGACGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAG152310.400163889647636No Hit
CTAGCTGTGACGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAG14249.72411909314395No Hit
CTAGCTGTGACGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAG12478.515432941819174No Hit
AGATCACTGTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGCACGGTGAG12158.296913411636165No Hit
AGATCACTGTCGGGTGGGAACACCTTGTTCAGGTCCTCGAGCACTGTCAG12058.228626058453974No Hit
AGATCACTGTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTGACCGTGAG9596.548757170172085No Hit
AGATCACTGTCGGGTGGGAACACCTTGTTCAGGTCCTCCAGCACTGAGAG6324.31576072111445No Hit
AGATCACTGTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAG5864.001638896476373No Hit
AGATCACTGTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAG4913.3529090412455615No Hit
AGATCACTGTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAG4192.8612400983337887No Hit
CTAGCTGTGACGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAG4002.7314941272876263No Hit
AGATCACTGTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGAG3532.41054356733133No Hit
CTAGCTGTGACGGGTGGGAACACGTTTTTCAGGTCCTCTACAACTGTGAG2871.9598470363288718No Hit
CTAGCTGTGACGGGTGGGAACACGTTTTTCAGGTCCTCTACAACAGTGAG2181.4886642993717563No Hit
CTAGCTGTGACGGGTGGGAACACGTTTTTCAGGTCCTCTAGGATGGAGAG2051.3998907402349083No Hit
CTAGCTGTGACGGGTGGGAACACGTTTTCAGGTCCTCTAGCACGGTGAGC1471.0038240917782026No Hit
CTAGCTGTGACGGGTGGGAACACGTTTTCAGGTCCTCGAGCACTGTCAGC1170.7989620322316306No Hit
CTAGCTGTGACGGGTGGGAACACGTTTTCAGGTCCTCTGTGACCGTGAGC1130.7716470909587545No Hit
AGATCACTGTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGTCAATATCT990.6760447965036875No Hit
CTAGCTGTGACGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAG970.6623873258672494No Hit
CTAGCTGTGACGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAG820.5599562960939634No Hit
AGATCACTGTCGGGTGGGAACACCTTGTTCAGGTCCTCGAGCACCAGGAG610.41655285441136297No Hit
CTAGCTGTGACGGGTGGGAACACGTTTTCAGGTCCTCTACAACTGTGAGT610.41655285441136297No Hit
CTAGCTGTGACGGGTGGGAACACGTTTTTCAGGTCCTCTGGTCAATATCT580.3960666484567058No Hit
CTAGCTGTGACGGGTGGGAACACGTTTTTTCAGGTCCTCGAGCACTGTCA510.3482655012291723No Hit
CTAGCTGTGACGGGTGGGAACACGTTTTCAGGTCCTCCAGCACTGAGAGC430.29363561868341986No Hit
CTAGCTGTGACGGGTGGGAACACGTTTTTTCAGGTCCTCTGGAAAGGGAA400.27314941272876264No Hit
CTAGCTGTGACGGGTGGGAACACGTTTTTTCAGGTCCTCTGTGACCGTGA370.2526632067741055No Hit
CTAGCTGTGACGGGTGGGAACACGTTTTCAGGTCCTCTAGGATGGAGAGT350.23900573613766732No Hit
AGATCACTGTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAG300.204862059546572No Hit
CTAGCTGTGACGGGTGGGAACACGTTTTTTCAGGTCCTCTAGCACGGTGA260.1775471182736957No Hit
AGATCACTGTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAG250.17071838295547664No Hit
GCTAGCTGTGACGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA250.17071838295547664No Hit
CTAGCTGTGACGGGTGGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA220.15023217700081945No Hit
CTAGCTGTGACGGGTGGGAACACGTTTTCAGGTCCTCTGGAAAGGGAAGA200.13657470636438132No Hit
CTAGCTGTGACGGGTGGGAACACGTTTTTTCAGGTCCTCTACAACAGTGA190.12974597104616223No Hit
GCTAGCTGTGACGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA180.12291723572794319No Hit
CTAGCTGTGACGGGTGGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA180.12291723572794319No Hit
CTAGCTGTGACGGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA160.10925976509150505No Hit
AGATCACTGTCGGGTGGGAACACCTTTGTTCAGGTCCTCTAGCACGGTGA160.10925976509150505No Hit
GAGATCACTGTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTGACCGTGA160.10925976509150505No Hit
GAGATCACTGTCGGGTGGGAACACCTTGTTCAGGTCCTCGAGCACTGTCA150.102431029773286No Hit
AGATCACTGTCGGGTGGGAACACCTTTGTTCAGGTCCTCGAGCACTGTCA150.102431029773286No Hit
GAGATCACTGTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGCACGGTGA150.102431029773286No Hit
CTAGCTGTGACGGGTGGGAACACGTTTTTTCAGGTCCTCTAGGATGGAGA150.102431029773286No Hit
CTAGCTGTGACGGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA150.102431029773286No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)