FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001775048

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001775048
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences442045
Sequences flagged as poor quality0
Sequence length55-451
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGCTCGTCCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAG6519314.748046013414923No Hit
ACTGCTCGTCCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAG4738710.719949326426043No Hit
ACTGCTCGTCCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAG401189.075546607245869No Hit
ACTGCTCGTCCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGT358978.120666447986064No Hit
ACTGCTCGTCCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAA303316.861518623669535No Hit
ACTGCTCGTCCGGGTGGGAACACCTTGTTCAGGTCCTCGAGCACTGTCAG209374.7363956158309675No Hit
ACTGCTCGTCCGGGTGGGAACACCTTGTTCAGGTCCTCTAGCACGGTGAG182274.123335859471321No Hit
ACTGCTCGTCCGGGTGGGAACACCTTGTTCAGGTCCTCTGTGACCGTGAG143253.240620298838354No Hit
ACTGCTCGTCCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAG140203.1716227985838548No Hit
ACTGCTCGTCCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAG115162.6051646325600335No Hit
ACTGCTCGTCCGGGTGGGAACACCTTGTTCAGGTCCTCTGGTCAATATCT100812.2805370493954236No Hit
ACTGCTCGTCCGGGTGGGAACACCTTGTTCAGGTCCTCCAGTACGGTCAG74251.679693243900508No Hit
ACTGCTCGTCCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAGAGGGGTTGGAGCCAGGGT45521.0297594136343584No Hit
ACTGCTCGTCCGGGTGGGAACACCTTGTTCAGGTCCTCGAGCACCAGGAG44241.0008030856586998No Hit
ACTGCTCGTCCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTTAG43930.993790224977095No Hit
AGTAGCGTATCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAG41310.9345202411519189No Hit
ACTGCTCGTCCGGGTGGGAACACCTTGTTCAGGTTAGGTATCAGCATATC38600.8732142655159543No Hit
AGTAGCGTATCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAG37940.8582836589035053No Hit
AGTAGCGTATCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAG33910.7671164700426427No Hit
ACTGCTCGTCCGGGTGGGAACACCTTGTTCAGGTCCTCCAGCACTGAGAG32470.7345406010700268No Hit
AGTAGCGTATCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAG29450.6662217647524573No Hit
ACTGCTCGTCCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGTCCTCAGGATGTTTTA20430.46217014104898824No Hit
AGTAGCGTATCGGGTGGGAACACGTTTTTCAGGTCCTCTACAACTGTGAG19940.45108529674580644No Hit
ACTGCTCGTCCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTCTGGTGCCTTGTCCA19740.44656087049960974No Hit
ACTGCTCGTCCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGAGCCAACTTCCCTCTCCAAAAT19610.4436199934395819No Hit
ACTGCTCGTCCGGGTGGGAACACCTTGTTCAGGTCCTATTTGTAATCCCA18830.425974731079415No Hit
ACTGCTCGTCCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGAG17810.40290015722381206No Hit
AGTAGCGTATCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTGT16840.3809566899297583No Hit
ACTGCTCGTCCGGGTGGGAACACCTTGTTCAGGTCCTAACTGTTAGGTAT16360.37009806693888636No Hit
ACTGCTCGTCCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGTCCTCAGGATGTTTTTTTTT15690.35494123901412755No Hit
AGTAGCGTATCGGGTGGGAACACGTTTTTCAGGTCCTCTAGGATGGAGAG15690.35494123901412755No Hit
ACTGCTCGTCCGGGTGGGAACACCTTGTTCAGGTCCTCCAGGCACTGTCC14280.3230440339784411No Hit
ACTGCTCGTCCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAC13240.2995170174982185No Hit
ACTGCTCGTCCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGAGCCAACTTCCCTCTCCAA12980.29363526337816287No Hit
ACTGCTCGTCCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTCGAGTCCCATCACCAAAATC12690.2870748453211777No Hit
ACTGCTCGTCCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATTAGGTA12100.2737277878948976No Hit
AGTAGCGTATCGGGTGGGAACACGTTTTTCAGGTCCTCTACAACGGTTAA12060.2728229026456583No Hit
ACTGCTCGTCCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACCTGGTCCCCGA11820.26739359115022227No Hit
ACTGCTCGTCCGGGTGGGAACACCTTGTTCAGGTCCTCTGGTCAATATCTGATGGGATGGTCCACA10980.24839100091619631No Hit
ACTGCTCGTCCGGGTGGGAACACCTTGTTCAGGTCCTCGAGCACTGTCAGCCGGGTGCCTGGGCCAAAAT10380.23481772217760635No Hit
AGTAGCGTATCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAG10330.2336866156160572No Hit
ACTGCTCGTCCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGGG9900.2239590991867344No Hit
ACTGCTCGTCCGGGTGGGAACACCTTGTTCAGGTCCTCTCTGCCCATCCC9870.22328043524980487No Hit
AGTAGCGTATCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAG9450.21377914013279192No Hit
ACTGCTCGTCCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGAGCCAACTTCCCTCTCCAAAATA7570.17124953341854335No Hit
ACTGCTCGTCCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGTCCTCAGGATGTTA7540.17057086948161387No Hit
ACTGCTCGTCCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGTCCTCAGGATGTTTTTTTTTTTTT7270.16446289404924838No Hit
ACTGCTCGTCCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAGCTGGGTTCCACTGCCAA7140.16152201698922056No Hit
ACTGCTCGTCCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGTCCTCAGGATGTTTTTTTTTTT6850.1549615989322354No Hit
ACTGCTCGTCCGGGTGGGAACACCTTGTTCAGGTCCTCTGTGACCGTGAGCCTGGTGCCCGGCCCGA6590.14907984481217976No Hit
ACTGCTCGTCCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGCTAG6290.14229320544288476No Hit
ACTGCTCGTCCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGTCCTCAGGATGTTTTGAGCTCCTT6280.14206698413057495No Hit
ACTGCTCGTCCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGTCCTCAGGATGTTTTTTTTTTTT5880.13301813163818163No Hit
ACTGCTCGTCCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGTCCTCAGGATGTTTTTTTT5760.13030347589046365No Hit
AGTAGCGTATCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAG5680.12849370539198499No Hit
AGTAGCGTATCGGGTGGGAACACGTTTTTCAGGTCCTCTGTCACAGTGAG5620.12713637751812598No Hit
ACTGCTCGTCCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGTCCTCAGGATGTTTTTTTTTT5370.12148084471038016No Hit
ACTGCTCGTCCGGGTGGGAACACCTTGTTCAGGTCCTCCAGCACGGTCAGCCTGCTGCCGT5290.1196710742119015No Hit
ACTGCTCGTCCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAGAGGGGTTGGAGCCAGGGC5280.11944485289959168No Hit
ACTGCTCGTCCGGGTGGGAACACCTTGTTCAGGTCCTCCACGACAGAGAG5260.118992410274972No Hit
ACTGCTCGTCCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAG4900.11084844303181803No Hit
ACTGCTCGTCCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGTCCTCAGGATGTTTTTTTTTTTTTT4580.10360936103790339No Hit
ACTGCTCGTCCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAG4500.10179959053942472No Hit
AGTAGCGTATCGGGTGGGAACACGTTTTTCAGGTCCTCCAAGACAGAGAG4490.10157336922711488No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)