FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001775050

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001775050
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences971975
Sequences flagged as poor quality0
Sequence length55-451
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTGTGACGCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAG921529.480902286581445No Hit
CAGTGTGACGCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAG891539.172355256050825No Hit
CAGTGTGACGCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGT743907.653489030067647No Hit
CACTCAGAGCCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAG446104.589624218729906No Hit
CAGTGTGACGCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAG432624.450937524113274No Hit
CACTCAGAGCCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAG355903.6616168111319736No Hit
CACTCAGAGCCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTGT350833.6094549756938195No Hit
CAGTGTGACGCGGGTGGGAACACCTTGTTCAGGTCCTCTAGCACGGTGAG285802.940404845803647No Hit
CAGTGTGACGCGGGTGGGAACACCTTGTTCAGGTACAGCGACCTCGGGTG265412.730625787700301No Hit
CACTCAGAGCCGGGTGGGAACACGTTTTTCAGGTCCTCTAGGATGGAGAG257842.6527431261092107No Hit
CACTCAGAGCCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAG226242.327631883536099No Hit
CACTCAGAGCCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAG221592.2797911468916383No Hit
CAGTGTGACGCGGGTGGGAACACCTTGTTCAGGTCCTCTGGTCAATATCT189801.9527251215309036No Hit
CAGTGTGACGCGGGTGGGAACACCTTGTTCAGGTCCTCGAGCACTGTCAG183401.8868798065793873No Hit
CACTCAGAGCCGGGTGGGAACACGTTTTTCAGGTACAGCGACCTCGGGTG178741.8389361866303147No Hit
CAGTGTGACGCGGGTGGGAACACCTTGTTCAGGTCCTCTGTGACCGTGAG164181.6891380951156152No Hit
CAGTGTGACGCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAG162021.6669153013194784No Hit
CAGTGTGACGCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGAG138691.4268885516602794No Hit
CACTCAGAGCCGGGTGGGAACACGTTTTTCAGGTCCTCTACAACTGTGAG123751.2731808945703336No Hit
CAGTGTGACGCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAA107811.1091849070192135No Hit
CACTCAGAGCCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAG94990.9772885105069575No Hit
CACTCAGAGCCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAG77100.7932302785565473No Hit
CAGTGTGACGCGGGTGGGAACACCTTGTTCAGGTCCTCGAGCACCAGGAG70120.7214177319375499No Hit
CAGTGTGACGCGGGTGGGAACACCTTGTTCAGGTCCTCCAGCACTGAGAG66640.6856143419326628No Hit
CACTCAGAGCCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAG59560.612772962267548No Hit
CAGTGTGACGCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAG53490.5503227963682193No Hit
CAGTGTGACGCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGTCCTCAGGATGTTTTA51920.5341701175441754No Hit
CAGTGTGACGCGGGTGGGAACACCTTGTTCAGGTCCTCCAGTACGGTCAG50520.5197664548985313No Hit
CACTCAGAGCCGGGTGGGAACACGTTTTTCAGGTCCTCTGGTCAATATCT49650.5108156073973096No Hit
CAGTGTGACGCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACCTGGTCCCCGAACCGAAGGTGT44920.4621518043159546No Hit
CACTCAGAGCCGGGTGGGAACACGTTTTTCAGGTCCTCCAAGACAGAGAG44260.45536150621157956No Hit
CAGTGTGACGCGGGTGGGAACACCTTGTTCAGGTCCTCTGGTCAATATCTGATGGGATGGTCCACAT42810.4404434270428766No Hit
CAGTGTGACGCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTTAG42810.4404434270428766No Hit
CACTCAGAGCCGGGTGGGAACACGTTTTTCAGGTCCTCTACAACAGTGAG37590.3867383420355462No Hit
CAGTGTGACGCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTCGAGTCCCATCACCAAAATTCA34410.35402145116901157No Hit
CACTCAGAGCCGGGTGGGAACACGTTTTTCAGGTCCTCTACAACGGTTAA33120.34074950487409655No Hit
CACTCAGAGCCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAG27450.28241467115923763No Hit
CACTCAGAGCCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTGTCCTCAGGATGTTTTA24240.24938913037886776No Hit
CAGTGTGACGCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAGAGGGG22890.2354998842562823No Hit
CAGTGTGACGCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGTCCTCAGGATGTTTT22610.23261915172715347No Hit
CACTCAGAGCCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCCGGGTGCCTGGGCCAAAAT22520.23169320198564777No Hit
CAGTGTGACGCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTCGAGTCCCATCACCAAAAT21740.22366830422593173No Hit
CAGTGTGACGCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGATAGATCT20700.21296844054631034No Hit
CACTCAGAGCCGGGTGGGAACACGTTTTTCAGGTCCTCTGTTAGATCTGT19870.2044291262635356No Hit
CAGTGTGACGCGGGTGGGAACACCTTGTTCAGGTCCTCGAGCACTGTCAGCCGGGTGCCTGGGCCAAAAT19370.19928496103294838No Hit
CAGTGTGACGCGGGTGGGAACACCTTGTTCAGGTCCTCTGTTAGATCTGT18710.19249466292857328No Hit
CAGTGTGACGCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGTCCTCAGGATGTTTTTC18320.18848221404871524No Hit
CAGTGTGACGCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTCTGGTGCCTT17910.1842639985596337No Hit
CAGTGTGACGCGGGTGGGAACACCTTGTTCAGGTCCTCGAGCACCAGGAGCCGCGTGCCTGGCCCGA17540.1804573162889992No Hit
CAGTGTGACGCGGGTGGGAACACCTTGTTCAGGTCCTCTGGTCAATTAGA17410.17911983332904652No Hit
CAGTGTGACGCGGGTGGGAACACCTTGTTCAGGTCCTCTGAAAGTTACAT17300.17798811697831735No Hit
CAGTGTGACGCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTTAG17240.1773708171506469No Hit
CACTCAGAGCCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCCGCGTGCCTGGCCCGA17160.17654775071375292No Hit
CACTCAGAGCCGGGTGGGAACACGTTTTTCAGGTCCTCTGTCACAGTGAG16370.16841996964942513No Hit
CAGTGTGACGCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTCGAGTCCCATCACCAAA15560.16008642197587386No Hit
CAGTGTGACGCGGGTGGGAACACCTTGTTCAGGTCCTCTGGTCATAGATC14910.1533990071761105No Hit
CAGTGTGACGCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGTCCTCAGGATGTTA14240.14650582576712362No Hit
CAGTGTGACGCGGGTGGGAACACCTTGTTCAGGTGCAGCGACCTCGGGTG13880.14280202680110085No Hit
CAGTGTGACGCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAT13840.14239049358265388No Hit
CAGTGTGACGCGGGTGGGAACACCTTGTTCAGGTCCTCCAATAGATCTGT13800.1419789603642069No Hit
CACTCAGAGCCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTTAG13440.1382751613981841No Hit
CACTCAGAGCCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCCTAGAGCCTTCTCCA13300.13683479513361968No Hit
CACTCAGAGCCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTGTCCTCAGGATGTTTTTC13160.13539442886905528No Hit
CACTCAGAGCCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTGTCCTCAGGATGTTTT12930.13302811286298516No Hit
CACTCAGAGCCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAGAGGTAATGGGGG12740.13107333007536204No Hit
CAGTGTGACGCGGGTGGGAACACCTTGTTCAGGTCCTCCAGTACGGTCAGCCTAGAGCCTTCTCCA12740.13107333007536204No Hit
CAGTGTGACGCGGGTGGGAACACCTTGTTCAGGTCCTCTGTGACCGTTAG12630.12994161372463284No Hit
CACTCAGAGCCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAG12470.1282954808508449No Hit
CACTCAGAGCCGGGTGGGAACACGTTTTTCAGGTCCTCTACAACGGTTAACCTGGTCCCCGAACCGAAGGTGT12240.12592916484477482No Hit
CACTCAGAGCCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTGTCCTCAGGATGTTTTGAGCTCCTT12180.12531186501710434No Hit
CAGTGTGACGCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGTA11890.12232824918336377No Hit
CAGTGTGACGCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAGAGGTAATGGGGG11730.12068211630957586No Hit
CAGTGTGACGCGGGTGGGAACACCTTGTTCAGGTCCTAGGATGGAGAGTC11650.11985904987268192No Hit
CAGTGTGACGCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGTCCTCAGGATGTTTTTTTTTT11520.11852156691272923No Hit
CAGTGTGACGCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGTCCTCAGGAA11480.11811003369428226No Hit
CACTCAGAGCCGGGTGGGAACACGTTTTTCAGGTCCTCTGGTCAATATCTGATGGGATGGTCCACAT11120.11440623472825948No Hit
CACTCAGAGCCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAG11070.11389181820520074No Hit
CAGTGTGACGCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGTCCTCAGGATGTTTTGAGCTCCTT11020.11337740168214203No Hit
CAGTGTGACGCGGGTGGGAACACCTTGTTCAGGTCCTCTATAGATCTGTA10940.11255433524524808No Hit
CACTCAGAGCCGGGTGGGAACACGTTTTTCAGGTCCTCTACAACTGTTAG10730.11039378584840145No Hit
CACTCAGAGCCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTGTCCTCAGGAAT10700.1100851359345662No Hit
CAGTGTGACGCGGGTGGGAACACCTTGTTCAGGTCCTCCAGCACGGTCAG10320.10617557035931995No Hit
CAGTGTGACGCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTCGAGTCCCATCACCAA10200.10494097070397901No Hit
CAGTGTGACGCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAC10180.10473520409475554No Hit
CAGTGTGACGCGGGTGGGAACACCTTGTTCAGGTACAGCGCCCTCGGGTG9990.10278042130713239No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)