FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001775052

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001775052
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13040
Sequences flagged as poor quality0
Sequence length40-578
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCTGTCAGCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAG10337.921779141104294No Hit
CGTCTGTCAGCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAG9447.2392638036809815No Hit
TGCGTCGAGTCGGGTGGGAACACCTTGTTCAGGTCCTCGAGCACTGTCAG9237.078220858895705No Hit
TGCGTCGAGTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGCACGGTGAG8956.863496932515338No Hit
CGTCTGTCAGCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAG5784.432515337423313No Hit
TGCGTCGAGTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAG5644.325153374233129No Hit
TGCGTCGAGTCGGGTGGGAACACCTTGTTCAGGTCCTCCAGTACGGTCAG4553.4892638036809815No Hit
CGTCTGTCAGCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAG4363.343558282208589No Hit
TGCGTCGAGTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAG3953.0291411042944785No Hit
CGTCTGTCAGCGGGTGGGAACACGTTTTTCAGGTCCTCTAGGATGGAGAG3943.021472392638037No Hit
TGCGTCGAGTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTGACCGTGAG3732.860429447852761No Hit
TGCGTCGAGTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAG3522.6993865030674846No Hit
TGCGTCGAGTCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGT3012.308282208588957No Hit
CGTCTGTCAGCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTGT2561.96319018404908No Hit
CGTCTGTCAGCGGGTGGGAACACGTTTTTCAGGTCCTCTACAACTGTGAG2441.871165644171779No Hit
CGTCTGTCAGCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAG2391.8328220858895703No Hit
CGTCTGTCAGCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAG2121.6257668711656441No Hit
TGCGTCGAGTCGGGTGGGAACACCTTGTTCAGGTCCTCGAGCACCAGGAG1731.3266871165644172No Hit
TGCGTCGAGTCGGGTGGGAACACCTTGTTCAGGTCCTAACTGTGAGTCTG1371.0506134969325154No Hit
CGTCTGTCAGCGGGTGGGAACACGTTTTCAGGTCCTCTCCAGGCACTGTC1210.9279141104294478No Hit
CGTCTGTCAGCGGGTGGGAACACGTTTTTCAGGTCCTCTACAACGGTTAA1170.897239263803681No Hit
TGCGTCGAGTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAA1160.8895705521472393No Hit
CGTCTGTCAGCGGGTGGGAACACGTTTTCAGGTCCTCGAGCACTGTCAGC1120.8588957055214724No Hit
CGTCTGTCAGCGGGTGGGAACACGTTTTTCAGGTCCTAACTGTGAGTCTG1010.7745398773006136No Hit
TGCGTCGAGTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGTCAATATCT1000.7668711656441718No Hit
CGTCTGTCAGCGGGTGGGAACACGTTTTTCAGGTCCTCTGGTCAATATCT710.5444785276073619No Hit
CGTCTGTCAGCGGGTGGGAACACGTTTTCAGGTCCTCTAGCACGGTGAGC680.5214723926380368No Hit
CGTCTGTCAGCGGGTGGGAACACGTTTTTTCAGGTCCTCGAGCACTGTCA580.4447852760736197No Hit
CGTCTGTCAGCGGGTGGGAACACGTTTTCAGGTCCTCTGTGACCGTGAGC520.39877300613496935No Hit
CGTCTGTCAGCGGGTGGGAACACGTTTTTTCAGGTCCTCTAGCACGGTGA460.35276073619631904No Hit
TGCGTCGAGTCGGGTGGGAACACCTTGTTCAGGTCCTCTCTAGGCCAGCC450.3450920245398773No Hit
TGCGTCGAGTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAG450.3450920245398773No Hit
CGTCTGTCAGCGGGTGGGAACACGTTTTTCAGGTCCTCCATTTTGTCTTC400.3067484662576687No Hit
TGCGTCGAGTCGGGTGGGAACACCTTGTTCAGGTCCTCCATTTTGTCTTC400.3067484662576687No Hit
TGCGTCGAGTCGGGTGGGAACACCTTGTTCAGGTCCTAACTGCTGTGCCA400.3067484662576687No Hit
CGTCTGTCAGCGGGTGGGAACACGTTTTCAGGTCCTCCAGTACGGTCAGC390.299079754601227No Hit
CGTCTGTCAGCGGGTGGGAACACGTTTTTCAGGTCCTCTGTCACAGTGAG370.2837423312883436No Hit
CGTCTGTCAGCGGGTGGGAACACGTTTTCAGGTCCTCTGGAAAGGGAAGA350.2684049079754601No Hit
TGCGTCGAGTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTCCT340.2607361963190184No Hit
CGTCTGTCAGCGGGTGGGAACACGTTTTTTCAGGTCCTCCAGTACGGTCA340.2607361963190184No Hit
CGTCTGTCAGCGGGTGGGAACACGTTTCAGGTCCTCGAGCACTGTCAGCC330.2530674846625767No Hit
CGTCTGTCAGCGGGTGGGAACACGTTTTTCAGGTCCTCTCTAGGCCAGCC330.2530674846625767No Hit
CGTCTGTCAGCGGGTGGGAACACGTTTTTCAGGTCCTCTACAACTGTCCT320.245398773006135No Hit
CGTCTGTCAGCGGGTGGGAACACGTTTCAGGTCCTCTCCAGGCACTGTCC320.245398773006135No Hit
CGTCTGTCAGCGGGTGGGAACACGTTTTCAGGTCCTCGAGCACCAGGAGC300.23006134969325154No Hit
CGTCTGTCAGCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAG300.23006134969325154No Hit
CGTCTGTCAGCGGGTGGGAACACGTTTTTTCAGGTCCTCTAGGATGGAGA280.2147239263803681No Hit
CGTCTGTCAGCGGGTGGGAACACGTTTTTCAGGTCCTCCAAGACAGAGAG240.18404907975460122No Hit
CGTCTGTCAGCGGGTGGGAACACGTTTTTTCAGGTCCTCTGTGACCGTGA240.18404907975460122No Hit
CGTCTGTCAGCGGGTGGGAACACGTTTTTCAGGTCCTAACTGCTGTGCCA210.1610429447852761No Hit
TGCGTCGAGTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAGGGAAGA190.14570552147239263No Hit
TGCGTCGAGTCGGGTGGGAACACCTTGTTCAGGTAAGCATAACCCTTACT190.14570552147239263No Hit
CGTCTGTCAGCGGGTGGGAACACGTTTCAGGTCCTCTAGCACGGTGAGCC180.13803680981595093No Hit
TGCGTCGAGTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAG170.1303680981595092No Hit
TGCGTCGAGTCGGGTGGGAACACCTTGTTCAGGTCCTCCAGCACGGTCAG160.1226993865030675No Hit
CGTCTGTCAGCGGGTGGGAACACGTTTTCAGGTCCTCTAGGATGGAGAGT160.1226993865030675No Hit
CGTCTGTCAGCGGGTGGGAACACGTTTTTTCAGGTCCTCTACAACTGTGA150.11503067484662577No Hit
CGTCTGTCAGCGGGTGGGAACACGTTTTTCAGGTCCTCTCTGCCCATCCC140.10736196319018405No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)