FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001775054

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001775054
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14121
Sequences flagged as poor quality0
Sequence length40-492
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTGAGAGACGGGTGGGAACACCTTGTTCAGGTCCTCTGGTCAATATCT215015.225550598399545No Hit
AGTCACATCACGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAG169812.024644147015085No Hit
CTGTGAGATGCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAG146110.346292755470577No Hit
CTGTGAGATGCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAG9846.968345018058211No Hit
AGTCACATCACGGGTGGGAACACCTTGTTCAGGTCCTCGAGCACTGTCAG9816.9471000637348626No Hit
ACGTGAGAGACGGGTGGGAACACCTTGTTCAGGTCCTCTAGCACGGTGAG8205.8069541817151755No Hit
CTGTGAGATGCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAG4513.193824799943347No Hit
AGTCACATCACGGGTGGGAACACCTTGTTCAGGTCCTCTGTGACCGTGAG3842.7193541533885703No Hit
ACGTGAGAGACGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGAG3562.5210679130373204No Hit
CTGTGAGATGCGGGTGGGAACACGTTTTCAGGTCCTCTGGAAAGGGAAGA2751.9474541463069188No Hit
ACGTGAGAGACGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAG2541.7987394660434814No Hit
ACGTGAGAGACGGGTGGGAACACCTTGTTCAGGTCCTCCAGCACTGAGAG2441.7279229516323207No Hit
ACGTGAGAGACGGGTGGGAACACCTTGTTCAGGTCCTCTGTGACCGTGAG1971.3950853338998654No Hit
AGTCACATCACGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAG1621.147227533460803No Hit
CTGTGAGATGCGGGTGGGAACACGTTTTCAGGTCCTCTGTGACCGTGAGC1571.1118192762552226No Hit
CTGTGAGATGCGGGTGGGAACACGTTTTTCAGGTCCTCTACAACTGTGAG1330.941859641668437No Hit
ACGTGAGAGACGGGTGGGAACACCTTGTTCAGGTCCTCGAGCACCAGGAG970.6869201897882586No Hit
ACGTGAGAGACGGGTGGAACACCTTGTTCAGGTCCTCTACAACAGTGAGC720.5098789037603569No Hit
CTGTGAGATGCGGGTGGGAACACGTTTTTTCAGGTCCTCGAGCACTGTCA700.49571560087812483No Hit
ACGTGAGAGACGGGTGGGAACACCTTGTTCAGGTACAGCGACCTCGGGTG640.4532256922314284No Hit
CTGTGAGATGCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAG570.4036541321436159No Hit
ACGTGAGAGACGGGTGGGAACACCTTGTTCAGGTAAGCATAACCCTTACT520.36824587493803557No Hit
AGTCACATCACGGGTGGGAACACCTTGTTCAGGTCCTCTAGCACGGTGAG510.36116422349691946No Hit
CAGTAGCGCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGTCAATATCT480.3399192691735713No Hit
CTGTGAGATGCGGGTGGGAACACGTTTTCAGGTCCTCTAGCACGGTGAGC430.30451101196799096No Hit
CTGTGAGATGCGGGTGGGAACACGTTTTTTCAGGTCCTCTGGAAAGGGAA400.28326605764464274No Hit
CTGTGAGATGCGGGTGGGAACACGTTTCAGGTCCTCTGTGACCGTGAGCC400.28326605764464274No Hit
ACGTGAGAGACGGGTGGGAACACCTTGTTCAGGTCCTCGAGCACTGTCAG390.27618440620352663No Hit
CTGTGAGATGCGGGTGGGAACACGTTTTCAGGTCCTCTACAACTGTGAGT390.27618440620352663No Hit
AGTCACATCACGGGTGGGAACACCTTGTTCAGGTCCTCTGGTCAATATCT360.25493945188017847No Hit
CTGTGAGATGCGGGTGGGAACACGTTTTCAGGTAAGCATAACCCTTACTT360.25493945188017847No Hit
CTGTGAGATGCGGGTGGGAACACGTTTTCAGGTCCTCGAGCACTGTCAGC350.24785780043906241No Hit
AGTCACATCACGGGTGGGAACACCTTGTTCAGGTACAGCGACCTCGGGTG330.23369449755683025No Hit
ACGTGAGAGACGGGTGGAACACCTTGTTCAGGTCCTCCAGCACTGAGAGC320.2266128461157142No Hit
CTGTGAGATGCGGGTGGGAACACGTTTTTCAGGTAAGCATAACCCTTACT310.2195311946745981No Hit
ACGTGAGAGACGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGT300.21244954323348203No Hit
CTGTGAGATGCGGGTGGGAACACGTTTTCAGGTACAGCGACCTCGGGTGG260.18412293746901778No Hit
CTGTGAGATGCGGGTGGGAACACGTTTTTCAGGTACAGCGACCTCGGGTG240.16995963458678565No Hit
CTGTGAGATGCGGGTGGGAACACGTTTCAGGTCCTCTGGAAGGGAAGAGG210.14871468026343743No Hit
CTGTGAGATGCGGGTGGGAACACGTTTCAGGTCCTCTGGAAAGGGAAGAG210.14871468026343743No Hit
CTGTGAGATGCGGGTGGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA200.14163302882232137No Hit
CTGTGAGATGCGGGTGGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAA190.13455137738120532No Hit
ACGTGAGAGACGGGTGGAACACCTTGTTCAGGTCCTCTGGTCAATATCTG180.12746972594008923No Hit
ACGTGAGAGACGGGTGGAACACCTTGTTCAGGTCCTCTAGCACGGTGAGC170.12038807449897317No Hit
ACGTGAGAGACGGGTGGAACACCTTGTTCAGGTCCTCTGTGACCGTGAGC170.12038807449897317No Hit
ACGTGAGAGACGGGTGGAACACCTTGTTCAGGTCCTCTGTGACCGTGAGT160.1133064230578571No Hit
AGTCACATCACGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGAGAAG150.10622477161674101No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)