FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001775055

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001775055
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14048
Sequences flagged as poor quality0
Sequence length40-554
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTACTAGACGGGTGGGAACACCTTGTTCAGGTCCTCGAGCACTGTCAG10937.780466970387244No Hit
TCAGCGACAGCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAG10477.453018223234624No Hit
TGCTACTAGACGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAG9276.598804100227791No Hit
TGCTACTAGACGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAG7585.395785876993166No Hit
TGCTACTAGACGGGTGGGAACACCTTGTTCAGGTCCTCTAGCACGGTGAG7485.324601366742597No Hit
TCAGCGACAGCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAG6134.3636104783599095No Hit
TGCTACTAGACGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAG5003.5592255125284735No Hit
TCAGCGACAGCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAG4673.3243166287015944No Hit
TCAGCGACAGCGGGTGGGAACACGTTTTTCAGGTCCTCTACAACTGTGAG4623.28872437357631No Hit
TGCTACTAGACGGGTGGGAACACCTTGTTCAGGTCCTCTGTGACCGTGAG4413.139236902050114No Hit
TCAGCGACAGCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAG4052.882972665148064No Hit
TCAGCGACAGCGGGTGGGAACACGTTTTTCAGGTCCTCTAGGATGGAGAG3342.3775626423690204No Hit
TGCTACTAGACGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGT2641.879271070615034No Hit
TGCTACTAGACGGGTGGGAACACCTTGTTCAGGTCCTCGAGCACCAGGAG2401.7084282460136675No Hit
TGCTACTAGACGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAA2391.7013097949886105No Hit
TCAGCGACAGCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAG2111.501993166287016No Hit
TGCTACTAGACGGGTGGGAACACCTTGTTCAGGTCCGAGCACCAGGAGCC1541.0962414578587698No Hit
TCAGCGACAGCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTGT1521.082004555808656No Hit
TCAGCGACAGCGGGTGGGAACACGTTTTTCAGGTCCTCTACAACGGTTAA1421.0108200455580867No Hit
TGCTACTAGACGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAG1360.9681093394077449No Hit
TCAGCGACAGCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAG1260.8969248291571754No Hit
TCAGCGACAGCGGGTGGGAACACGTTTTCAGGTCCTCGAGCACTGTCAGC1250.8898063781321184No Hit
TCAGCGACAGCGGGTGGGAACACGTTTTTCAGGTCCGAGCACCAGGAGCC1230.8755694760820045No Hit
TGCTACTAGACGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAG1220.8684510250569476No Hit
TGCTACTAGACGGGTGGGAACACCTTGTTCAGGTCCTCCAGCACTGAGAG1150.818621867881549No Hit
TCAGCGACAGCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAG1130.8043849658314352No Hit
TGCTACTAGACGGGTGGGAACACCTTGTTCAGGTCCTCCAGTACGGTCAG970.6904897494305239No Hit
TCAGCGACAGCGGGTGGGAACACGTTTTCAGGTCCTCTGTGACCGTGAGC770.5481207289293849No Hit
TGCTACTAGACGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGAG760.541002277904328No Hit
TCAGCGACAGCGGGTGGGAACACGTTTTCAGGTCCTCTAGGATGGAGAGT710.5054100227790433No Hit
TCAGCGACAGCGGGTGGGAACACGTTTTCAGGTCCTCTCCAGGCACTGTC630.4484624145785877No Hit
TCAGCGACAGCGGGTGGGAACACGTTTTCAGGTCCTCTAGCACGGTGAGC600.4271070615034169No Hit
TCAGCGACAGCGGGTGGGAACACGTTTTTCAGGTCCTCCAAGACAGAGAG590.41998861047835995No Hit
TCAGCGACAGCGGGTGGGAACACGTTTTTCAGGTCCTCTGTCACAGTGAG580.41287015945330297No Hit
TCAGCGACAGCGGGTGGGAACACGTTTTCAGGTCCTCTGGAAAGGGAAGA490.3488041002277904No Hit
TCAGCGACAGCGGGTGGGAACACGTTTCAGGTCCTCGAGCACTGTCAGCC450.32033029612756264No Hit
TCAGCGACAGCGGGTGGGAACACGTTTTTTCAGGTCCTCTAGCACGGTGA440.31321184510250566No Hit
TGCTACTAGACGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATCCTAAC430.30609339407744873No Hit
TCAGCGACAGCGGGTGGGAACACGTTTTCAGGTCCTCTACAACTGTGAGT370.26338268792710706No Hit
TCAGCGACAGCGGGTGGGAACACGTTTTTCAGGTCCTCTACAACAGTGAG340.24202733485193623No Hit
TCAGCGACAGCGGGTGGGAACACGTTTCAGGTCCTCTAGGATGGAGAGTC330.23490888382687924No Hit
TCAGCGACAGCGGGTGGGAACACGTTTTTTCAGGTCCTCGAGCACTGTCA310.22067198177676536No Hit
TCAGCGACAGCGGGTGGGAACACGTTTTTCAGGTCCTGCTGGATAGCCTC270.1921981776765376No Hit
TCAGCGACAGCGGGTGGGAACACGTTTCAGGTCCTCTCCAGGCACTGTCC260.18507972665148065No Hit
TGCTACTAGACGGGTGGGAACACCTTGTTCAGGTAAGCATAACCCTTACT250.1779612756264237No Hit
TGCTACTAGACGGGTGGGAACACCTTGTTCAGGTCCTGCTGGATAGCCTC240.17084282460136674No Hit
TCAGCGACAGCGGGTGGGAACACGTTTTCAGGTCCTCGAGCACCAGGAGC240.17084282460136674No Hit
TCAGCGACAGCGGGTGGGAACACGTTTTTCAGGTCCTCGTATGCTACTCT230.1637243735763098No Hit
TCAGCGACAGCGGGTGGGAACACGTTTTCAGGTCCGAGCACCAGGAGCCG220.15660592255125283No Hit
TGCTACTAGACGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAGGGAAGA220.15660592255125283No Hit
TCAGCGACAGCGGGTGGGAACACGTTTTTTCAGGTCCTCTGGAAAGGGAA220.15660592255125283No Hit
TCAGCGACAGCGGGTGGGAACACGTTTTTTCAGGTCCTCTGTGACCGTGA220.15660592255125283No Hit
TCAGCGACAGCGGGTGGGAACACGTTTTTCTAGGTCCTCGAGCACTGTCA210.1494874715261959No Hit
TGCTACTAGACGGGTGGGAACACCTTGTTCAGGTCCTCGTATGCTACTCT210.1494874715261959No Hit
TCAGCGACAGCGGGTGGGAACACGTTTCAGGTCCTCTAGCACGGTGAGCC210.1494874715261959No Hit
TCAGCGACAGCGGGTGGGAACACGTTTCAGGTCCTCTGTGACCGTGAGCC190.135250569476082No Hit
GTCAGCGACAGCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA170.12101366742596811No Hit
TCAGCGACAGCGGGTGGGAACACGTTTCAGGTCCTCTACAACTGTGAGTC160.11389521640091116No Hit
TGCTACTAGACGGGTGGGAACACCTTGTTCAGGTCCTCTGGTCAATATCT160.11389521640091116No Hit
TGCTACTAGACGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAN160.11389521640091116No Hit
TCAGCGACAGCGGGTGGGAACACGTTTTTTCAGGTCCTCTAGGATGGAGA150.10677676537585422No Hit
TCAGCGACAGCGGGTGGGAACACGTTTCAGGTCCGAGCACCAGGAGCCGC150.10677676537585422No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)