FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001775057

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001775057
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20294
Sequences flagged as poor quality0
Sequence length40-541
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACATCAGTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAG15697.731349167241549No Hit
CGCTAGATCACGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAG12686.248152163200946No Hit
ATACATCAGTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAG11155.494234749186952No Hit
ATACATCAGTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAG8414.14408199467823No Hit
CTCAGTCGTACGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAG7853.8681383660195134No Hit
CTCAGTCGTACGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAG7813.8484281068296053No Hit
CTACAGAGCGCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAG6783.340888932689465No Hit
CTACAGAGCGCGGGTGGGAACACCTTGTTCAGGTCCTCGAGCACCAGGAG6753.326106238297034No Hit
CTCAGTCGTACGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAG5792.8530600177392333No Hit
CTACAGAGCGCGGGTGGGAACACCTTGTTCAGGTCCTCTGTGACCGTGAG5662.789001675372031No Hit
CTCAGTCGTACGGGTGGGAACACGTTTTTCAGGTCCTCTACAACTGTGAG5532.724943333004829No Hit
CTACAGAGCGCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAG5422.670740120232581No Hit
CTACAGAGCGCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAG3911.9266778358135408No Hit
ATACATCAGTCGGGTGGGAACACGTTTTTCAGGTCCTCTACAACGGTTAA3821.882329752636247No Hit
CTCAGTCGTACGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAG3641.7936335862816597No Hit
CGCTAGATCACGGGTGGGAACACCTTGTTCAGGTCCTCTGTGACCGTGAG3581.7640681974967969No Hit
ATACATCAGTCGGGTGGGAACACGTTTTCAGGTCCTCTGGAAAGGGAAGA3201.5768207351926677No Hit
CTACAGAGCGCGGGTGGGAACACCTTGTTCAGGTCCTCTAGCACGGTGAG2831.3945008376860155No Hit
CTCAGTCGTACGGGTGGGAACACGTTTTTCAGGTCCTCTAGGATGGAGAG2651.305804671331428No Hit
CGCTAGATCACGGGTGGGAACACCTTGTTCAGGTCCTCGAGCACTGTCAG2601.2811668473440425No Hit
ATACATCAGTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGGATGGAGAG2371.1678328570020695No Hit
CTCAGTCGTACGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTGT2231.0988469498373903No Hit
CTACAGAGCGCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGT2141.0544988666600965No Hit
CTACAGAGCGCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAG2020.9953680890903714No Hit
ATACATCAGTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAG1570.7736276732039026No Hit
CTCAGTCGTACGGGTGGGAACACGTTTTTTCAGGTCCTCTGGAAAGGGAA1550.7637725436089484No Hit
ATACATCAGTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAG1500.739134719621563No Hit
CTCAGTCGTACGGGTGGGAACACGTTTTTCAGGTCCTCTGTCACAGTGAG1480.7292795900266089No Hit
CTACAGAGCGCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAA1420.6997142012417463No Hit
ATACATCAGTCGGGTGGGAACACGTTTTCAGGTCCTCTGTGACCGTGAGC1360.6701488124568838No Hit
CGCTAGATCACGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAA1190.5863802108997733No Hit
CGCTAGATCACGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAG1130.5568148221149107No Hit
CTCAGTCGTACGGGTGGGAACACGTTTTTCAGGTCCTCTACAACGGTTAA1090.5371045629250025No Hit
CTCAGTCGTACGGGTGGGAACACGTTTTCAGGTCCTCTCCAGGCACTGTC1080.5321769981275254No Hit
CTACAGAGCGCGGGTGGGAACACCTTGTTCAGGTAAGCATAACCCTTACT860.42377057258302947No Hit
CTCAGTCGTACGGGTGGGAACACGTTTTCAGGTCCTCTGTGACCGTGAGC830.4089878781905982No Hit
CTACAGAGCGCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAACCTAAC740.36463979501330446No Hit
ATACATCAGTCGGGTGGGAACACGTTTTCAGGTCCTCGAGCACTGTCAGC730.3597122302158274No Hit
ATACATCAGTCGGGTGGGAACACGTTTCAGGTCCTCTGTGACCGTGAGCC710.34985710062087316No Hit
CTCAGTCGTACGGGTGGGAACACGTTTTTTCAGGTCCTCGAGCACCAGGA600.2956538878486252No Hit
ATACATCAGTCGGGTGGGAACACGTTTCAGGTCCTCTGGAAAGGGAAGAG600.2956538878486252No Hit
CTCAGTCGTACGGGTGGGAACACGTTTTCAGGTCCTCTACAACGGTTAAC560.27594362865871686No Hit
CGCTAGATCACGGGTGGGAACACCTTGTTCAGGTAAGCATAACCCTTACT530.2611609342662856No Hit
ATACATCAGTCGGGTGGGAACACGTTTCAGGTCCTCTGGAAGGGAAGAGG520.25623336946880854No Hit
CGCTAGATCACGGGTGGGAACACCTTGTTCAGGTCCTCCAGTACGGTCAG510.2513058046713314No Hit
ATACATCAGTCGGGTGGGAACACGTTTTTTCAGGTCCTCGAGCACTGTCA500.24637823987385432No Hit
ATACATCAGTCGGGTGGGAACACGTTTTTCAGGTAAGCATAACCCTTACT500.24637823987385432No Hit
ATACATCAGTCGGGTGGGAACACGTTTTTTCAGGTCCTCTGTGACCGTGA500.24637823987385432No Hit
ATACATCAGTCGGGTGGGAACACGTTTTTCAGGTCCTCTACAACTGTGAG500.24637823987385432No Hit
CTCAGTCGTACGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAG500.24637823987385432No Hit
CTCAGTCGTACGGGTGGGAACACGTTTTCAGGTCCTCTGGAAAGGGAAGA490.24145067507637727No Hit
ATACATCAGTCGGGTGGGAACACGTTTTTTCAGGTCCTCTGGAAAGGGAA480.23652311027890013No Hit
CTCAGTCGTACGGGTGGGAACACGTTTTCAGGTCCTCTAGGATGGAGAGT470.23159554548142308No Hit
CGCTAGATCACGGGTGGGAACACCTTGTTCAGGTCCTCGAGCACCAGGAG460.226667980683946No Hit
CTCAGTCGTACGGGTGGGAACACGTTTTCAGGTCCTCGAGCACCAGGAGC450.22174041588646892No Hit
CTCAGTCGTACGGGTGGGAACAGTTTTTCAGGTGATTCGTATTGGAAAAC440.21681285108899181No Hit
CTCAGTCGTACGGGTGGGAACACGTTTTTCAGGTAAGCATAACCCTTACT410.20203015669656055No Hit
CTACAGAGCGCGGGTGGGAACACCTTGTTCAGGTCCTCCAGCACGGTCAG380.1872474623041293No Hit
CTCAGTCGTACGGGTGGGAACACGTTTTTTCAGGTCCTCTGTGACCGTGA360.17739233270917512No Hit
CTCAGTCGTACGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAG340.16753720311422096No Hit
ATACATCAGTCGGGTGGGAACACGTTTTCAGGTAAGCATAACCCTTACTT300.1478269439243126No Hit
CTCAGTCGTACGGGTGGGAACACGTTTTCAGGTCCTCTACAACTGTGAGT300.1478269439243126No Hit
CTCAGTCGTACGGGTGGGAACACGTTTTCAGGTCCTCTAGCACGGTGAGC280.13797181432935843No Hit
CTCAGTCGTACGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAACCTAAC280.13797181432935843No Hit
CGCTAGATCACGGGTGGGAACACCTTGTTCAGGTACAGCGACCTCGGGTG270.13304424953188135No Hit
CTCAGTCGTACGGGTGGGAACACGTTTTCAGGTAAGCATAACCCTTACTT260.12811668473440427No Hit
CTCAGTCGTACGGGTGGGAACACGTTTTTTCAGGTCCTCTAGCACGGTGA260.12811668473440427No Hit
CTCAGTCGTACGGGTGGGAACACGTTTTCAGGTCCTCCAGCACGGTCAGC250.12318911993692716No Hit
ATACATCAGTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAG250.12318911993692716No Hit
CTCAGTCGTACGGGTGGGAACACGTTTTTTCAGGTCCTCTAGGATGGAGA240.11826155513945007No Hit
ATACATCAGTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAN240.11826155513945007No Hit
CTCAGTCGTACGGGTGGGAACACGTTTTTTCAGGTCCTCTCCAGGCACTG240.11826155513945007No Hit
ATACATCAGTCGGGTGGGAACACGTTTTCAGGTCCTCGAGCACTGTCANC240.11826155513945007No Hit
ATACATCAGTCGGGTGGGAACACGTTTTTCAGGTACAGCGACCTCGGGTG230.113333990341973No Hit
CTCAGTCGTACGGGTGGGAACACGTTTTTTCAGGTCCTCTGTCACAGTGA220.10840642554449591No Hit
ATACATCAGTCGGGTGGGAACACGTTTTTCTAGGTCCTCTGTGACCGTGA210.10347886074701883No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)