FastQCFastQC Report
Thu 5 Jan 2023
EGAF00001789806

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001789806
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119473417
Sequences flagged as poor quality0
Sequence length20-50
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATT3688900.3087632456348009No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC3147980.2634879020828541No Hit
CGGTTAAAACGGTGAAATTTCGTTTTTATTAAAAATATAAAAAATTAGTT3010100.2519472595313818No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATT2660330.22267129097010757No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTTTTAGTT2359400.19748326106718786No Hit
GATCGGAAGAGCACACGTCTGAACTCCAG2295020.19209461465390246Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGATGGTTTCGATTTTTTGATTTCGTGATTCGTTCGTTTCGGTTTTTT1841560.1541397280032595No Hit
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGT1743220.14590860827224855No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT1389190.11627607503684272No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)