FastQCFastQC Report
Mon 4 Sep 2023
EGAF00001789818

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001789818
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102328865
Sequences flagged as poor quality0
Sequence length20-50
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATT4144950.40506166075427497No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATT3331410.3255591665167008No Hit
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGT2928290.28616461249716785No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC2766430.2703469837176441No Hit
GATCGGAAGAGCACACGTCTGAACTCCAG2301880.2249492359755969Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGCGCGGTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGT1974340.19294067221404243No Hit
CGGGATGGTTTCGATTTTTTGATTTCGTGATTCGTTCGTTTCGGTTTTTT1770410.17301178900010275No Hit
CGGTTAAAACGGTGAAATTTCGTTTTTATTAAAAATATAAAAAATTAGTT1634480.15972814708733454No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTTTTAGTT1513680.147923071364077No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATT1259640.12309723165599461No Hit
CGGGCGTGGTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGT1149180.11230262350706226No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)