FastQCFastQC Report
Mon 27 Mar 2023
EGAF00001789819

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001789819
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102328865
Sequences flagged as poor quality0
Sequence length20-50
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATTCACGCCATTCTCCTACCTCAACCTCCCGAATAACTAAAACTACA6525170.6376666056053686No Hit
CAAATTCACGCCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACA4992820.48791902460757286No Hit
CAAACGCGATAACTCACGCCTATAATCCCAACACTTTAAAAAACCGAAAC3167970.30958713360106166No Hit
CAAATTCACACCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACA2305020.22525608976509218No Hit
CAAACGTAATAACGAACGCCTATAATCCCAACTACTTAAAAAACTAAAAC2289070.22369738978342035No Hit
GACTGGAGTTCAGACGTGTGCTCTTCCGAT1923180.18794110537627873Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAACTAAAACGATAAAACCCCGTCTCTACTAAAAATACAAAAAATTAACC1578070.15421552853146567No Hit
CAACTAATTTTTTATATTTTTAATAAAAACGAAATTTCACCGTTTTAACC1526400.1491661223839432No Hit
CAAACGTAATAACGAACGCCTATAATCCCAACTACTCGAAAAACTAAAAC1425180.13927448525887587No Hit
CAAATTCACACCATTCTCCTACCTCAACCTCCCGAATAACTAAAACTACA1389230.13576130254156538No Hit
CAACTAATTTTTTATATTTTTAATAAAAACGAAATTTCACCGTATTAACC1202050.11746929861872309No Hit
CAAACGCGATAACGAACGCCTATAATCCCAACTACTCGAAAAACTAAAAC1156800.11304728142934059No Hit
CAAATTCACGCCATTCTCCTACCTCAACCTCCTAAATAACTAAAACTACA1154720.11284401522483416No Hit
CAAATAATTCCATTCCATTACATTCCATTCCATTCCATTCCCCTACACTC1152560.11263293108938519No Hit
CAAAATAATCTCGATCTCCTAACCTCGTAATCCGCCCGCCTCGACCTCCC1087750.10629942978454808No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)