FastQCFastQC Report
Fri 24 Mar 2023
EGAF00001789888

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001789888
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125883890
Sequences flagged as poor quality0
Sequence length20-50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC4199900.33363284213730604No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATT4022430.3195349301646144No Hit
GATCGGAAGAGCACACGTCTGAACTCCAG3167520.25162234818132806Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATT2851380.22650872959200738No Hit
CGGTTAAAACGGTGAAATTTCGTTTTTATTAAAAATATAAAAAATTAGTT2718290.21593628859101827No Hit
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGT2560900.2034334973283714No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTTTTAGTT2092600.16623254969321333No Hit
CGGGATGGTTTCGATTTTTTGATTTCGTGATTCGTTCGTTTCGGTTTTTT1991370.15819101236862002No Hit
CGGGCGCGGTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGT1676630.13318860737462115No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATT1417860.11263236304502507No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)