FastQCFastQC Report
Mon 3 Apr 2023
EGAF00001789896

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001789896
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109700825
Sequences flagged as poor quality0
Sequence length20-50
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAG5902900.5380907572937579Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATT4018640.36632723591641175No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC2930320.2671192308717824No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATT2792670.2545714674433852No Hit
CGGTTAAAACGGTGAAATTTCGTTTTTATTAAAAATATAAAAAATTAGTT2569520.23422977903766903No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTTTTAGTT2075630.18920823977394882No Hit
CGGGATGGTTTCGATTTTTTGATTTCGTGATTCGTTCGTTTCGGTTTTTT1639840.14948292321411438No Hit
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGT1608940.14666617138020613No Hit
GATCGGAAGAGCACACGTCTGAACTCCGG1541160.14048754874906366Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATT1314880.1198605388792655No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATG1208880.1101978950477355No Hit
CGGGCGCGGTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGT1146570.1045179013011069No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)