FastQCFastQC Report
Sun 8 Jan 2023
EGAF00001789897

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001789897
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109700825
Sequences flagged as poor quality0
Sequence length20-50
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTGGAGTTCAGACGTGTGCTCTTCCGAT4960530.45218711892093794Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAAATTCACGCCATTCTCCTACCTCAACCTCCCGAATAACTAAAACTACA3140940.28631872185099794No Hit
CAAATAATTCCATTCCATTACATTCCATTCCATTCCATTCCCCTACACTC2650280.24159161975308754No Hit
CAACTAATTTTTTATATTTTTAATAAAAACGAAATTTCACCGTTTTAACC2533010.23090163633682792No Hit
CAAACGCGATAACTCACGCCTATAATCCCAACACTTTAAAAAACCGAAAC2115660.1928572551756106No Hit
CAACTAAAACGATAAAACCCCGTCTCTACTAAAAATACAAAAAATTAACC2080580.18965946700947783No Hit
CAAATTCACGCCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACA1917150.17476167567563872No Hit
GACCGGAGTTCAGACGTGTGCTCTTCCGAT1490330.13585403756079317Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CAAACGTAATAACGAACGCCTATAATCCCAACTACTTAAAAAACTAAAAC1321440.12045852891261301No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)