FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001790226

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001790226
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24147060
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20410718.452668772098964No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2325000.9628501357929288No Hit
CTCCGGGCGGACTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCAC2315500.9589159094316244No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1886180.7811220082279168No Hit
CGGGCGGACTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCACGCC1804120.7471385750480597No Hit
CCGGGCGGACTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCACGC1670700.6918854717717188No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1439340.5960725653557825No Hit
CCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTC1256420.5203200720915921No Hit
CCGGCCTTCACCTTCATTGCGCCACGGCGGCTTTCGTGCGAGCCCCCGAC1252530.5187091099289106No Hit
CCCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCAT1159000.47997561607914174No Hit
TCCGGGCGGACTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCACG1064970.441035057684041No Hit
CACCTTCATTGCGCCACGGCGGCTTTCGTGCGAGCCCCCGACTCGCGCAC981410.40643043086818853No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA950280.3935385922758298No Hit
GGACATAGTAATAACAATTGAATGTCTGCACAGCCACTTTCCACACAGAC863270.3575052200971878No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTTGCGCCGGTCCA776040.32138073951859975No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT761830.3154959651402697No Hit
CGGCCTTCACCTTCATTGCGCCACGGCGGCTTTCGTGCGAGCCCCCGACT723060.29944018029524094No Hit
GGGCGGACTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCACGCCC719440.29794103298703856No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTCTAATACTGGTG599330.24819998790743056No Hit
GCTTGTAGGACATAGTAATAACAATTGAATGTCTGCACAGCCACTTTCCA594490.2461956031086186No Hit
CCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT588020.24351618789202495No Hit
CTTCACCTTCATTGCGCCACGGCGGCTTTCGTGCGAGCCCCCGACTCGCG543430.2250501717393339No Hit
GCCTTCACCTTCATTGCGCCACGGCGGCTTTCGTGCGAGCCCCCGACTCG505470.2093298314577427No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT497740.20612861358691287No Hit
GCGGAGCGAGCGCACGGGGTCGGCGGCGACGTCGGCTACCCACCCGACCC495950.20538732251462496No Hit
GCCGACACGGCCGGACCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCG491340.20347818740666565No Hit
CCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCGCGGCGCACCGCCG478860.1983098563551836No Hit
GCCCATTTGAGTATTTTGTTTTCAATTAGGGAGATAGTTGGTATTAGGAT475950.19710474070135245No Hit
CCCCGCTTCGCGCCCCAGCCCGACCGACCCAGCCCTTAGAGCCAATCCTT462050.19134834634112807No Hit
GGGGAGATAGGTAGGAGTAGCGTGGTAAGGGCGATGAGTGTGGGGAGGAA459320.19021777392361638No Hit
GGCCTTCACCTTCATTGCGCCACGGCGGCTTTCGTGCGAGCCCCCGACTC449990.18635394950772474No Hit
ATTCGGTTCAGTCTAATCCTTTTTGTAGTCACTCATAGGCCAGACTTAGGGCTAGGATGATGATTAATAAGAGGG441530.18285041740071048No Hit
CCTTCACCTTCATTGCGCCACGGCGGCTTTCGTGCGAGCCCCCGACTCGC439030.18181509467405144No Hit
CCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT434600.17998050280241157No Hit
GTGGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTT433760.17963263436625412No Hit
GGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTG429380.17781874894914743No Hit
CTTCATTGCGCCACGGCGGCTTTCGTGCGAGCCCCCGACTCGCGCACGTGTTAGACTCCTTGGTCCGTGTTACA419330.173656751587978No Hit
CCCGGGCGGACTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCACG410150.16985504653568592No Hit
GTTCGTCCAAGTGCACTTTCCAGTACACTTACCATGTTACGACTTGTCTCCTCTATATAAATGCGTAGGGGTTTT388200.16076491299561935No Hit
CGGGCGGACTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTC384370.15917879857837766No Hit
GTCGGGTGGGTAGCCGACGTCGCCGCCGACCCCGTGCGCTCGCTCCGCCG379860.1573110763794847No Hit
CCCTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAA376410.15588233101669519No Hit
GCGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGACCGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCC374430.15506235541718122No Hit
GTAGGACATAGTAATAACAATTGAATGTCTGCACAGCCACTTTCCACACAGACATCATAACAAAAAATTTCCACC365580.15139731296480813No Hit
CTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATGCCAGTATCAGGCGGCGGCTTCGA362560.150146643111004No Hit
CCGGTAATGATGTCGGGGTTGAGGGATAGGAGGAGAATGGGGGATAGGTG357000.14784408536691424No Hit
GTGTTGTCGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTAGG356390.14759146662160944No Hit
GCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGT355030.14702825105830689No Hit
GCCGGCCTTCACCTTCATTGCGCCACGGCGGCTTTCGTGCGAGCCCCCGA343370.14219950586116903No Hit
CCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT340980.14120973733448297No Hit
GTTCAGTCTAATCCTTTTTGTAGTCACTCATAGGCCAGACTTAGGGCTAGGATGATGATTAATAAGAGGGATGAC335670.1390107118630591No Hit
CGCCGACACGGCCGGACCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTTTACCTCTTA331770.13739560840947096No Hit
GTCTGCACAGCCACTTTCCACACAGACATCATAACAAAAAATTTCCACCA330460.1368530993007016No Hit
CCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGT329020.136256753410146No Hit
GCCCCGCTTCGCGCCCCAGCCCGACCGACCCAGCCCTTAGAGCCAATCCT322050.13337027364822052No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT312490.12941119954147626No Hit
AGAACTTTGAAGAGAGAGTTCAAGAGGGCGTGAAACCGTTAAGAGGTAAA303240.12558050545283772No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG295530.12238757016382118No Hit
CCCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT287690.11914079809301836No Hit
GGGAGATAGGTAGGAGTAGCGTGGTAAGGGCGATGAGTGTGGGGAGGAAT284950.11800608438460004No Hit
GGTCGGGTGGGTAGCCGACGTCGCCGCCGACCCCGTGCGCTCGCTCCGCC275480.1140842818960155No Hit
CTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATT275380.11404286898694914No Hit
GGCAGGTTAGTTGTTTGTAGGGCTCATGGTAGGGGTAAAAGGAGGGCAATTTCTAGATCAAATAATAAGAAGGTA271090.11226625518800219No Hit
GGCGAACATACTTACTAAAGTGTATTAATTAATTAATGCTTGTAGGACATAGTAATAACAATTGAATGTCTGCAC268500.1111936608431834No Hit
GCCCCTATACCCAGGTCGGACGACCGATTTGCACGTCAGGACCGCTACGG262190.10858050628109592No Hit
CCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCGCGGCGCACCGCCGC257760.10674591440945605No Hit
CCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCG255710.10589694977359562No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA254550.10541656002842582No Hit
GTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCA252420.1045344650653123No Hit
AAAGAACTTTGAAGAGAGAGTTCAAGAGGGCGTGAAACCGTTAAGAGGTA251200.10402922757470268No Hit
GGACGGGGCCCCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCC250930.10391741272022349No Hit
CTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGT245530.10168111563063993No Hit
GGCTCTAGAGGGGGTAGAGGGGGTGCTATAGGGTAAATACGGGCCCTATT242000.10021923994059732No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)