FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001790227

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001790227
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24147060
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20314338.412755010340803No Hit
GCGGAGCGAGCGCACGGGGTCGGCGGCGACGTCGGCTACCCACCCGACCC3333071.3803212482182095No Hit
GTTGAAAAGAACTTTGAAGAGAGAGTTCAAGAGGGCGTGAAACCGTTAAG2200230.9111792491508283No Hit
AGAACTTTGAAGAGAGAGTTCAAGAGGGCGTGAAACCGTTAAGAGGTAAA2020600.8367892405949213No Hit
GAACTTTGAAGAGAGAGTTCAAGAGGGCGTGAAACCGTTAAGAGGTAAAC1472040.609614586620483No Hit
GGCGGAGCGAGCGCACGGGGTCGGCGGCGACGTCGGCTACCCACCCGACC1467080.6075605063307914No Hit
AAAGAACTTTGAAGAGAGAGTTCAAGAGGGCGTGAAACCGTTAAGAGGTA1294050.5359037497732643No Hit
GCGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGACCGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCC1172750.4856698910757666No Hit
CTGGTGTTAGGGTTCTTTGTTTTTGGGGTTTGGCAGAGATGTGTTTAAGT1094640.4533222678040308No Hit
CCCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT929140.3847839032992008No Hit
GAAAAGAACTTTGAAGAGAGAGTTCAAGAGGGCGTGAAACCGTTAAGAGG796450.3298331142590444No Hit
TGAAAAGAACTTTGAAGAGAGAGTTCAAGAGGGCGTGAAACCGTTAAGAG773960.32051935101001944No Hit
TTGAAAAGAACTTTGAAGAGAGAGTTCAAGAGGGCGTGAAACCGTTAAGA750250.3107003502703849No Hit
AAAAGAACTTTGAAGAGAGAGTTCAAGAGGGCGTGAAACCGTTAAGAGGT749990.31059267670681234No Hit
GCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCG703140.2911907288092215No Hit
GCTGGTGTTAGGGTTCTTTGTTTTTGGGGTTTGGCAGAGATGTGTTTAAG624630.25867745390122027No Hit
CGGAGCGAGCGCACGGGGTCGGCGGCGACGTCGGCTACCCACCCGACCCGTCTTGTAACACGGACCAAGGAGTCT527450.21843238887052918No Hit
CGGCGGAGCGAGCGCACGGGGTCGGCGGCGACGTCGGCTACCCACCCGAC516440.21387282758232265No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA464250.19225943034058804No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC451050.1867929263438282No Hit
CGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGACCGTCCCCCGACCGGCGA437510.1811856184562427No Hit
TGGAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAACTATGCCTG406030.16814883468215178No Hit
AAGAACTTTGAAGAGAGAGTTCAAGAGGGCGTGAAACCGTTAAGAGGTAA403410.16706381646461307No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT395890.16394956570282262No Hit
GGAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAACTATGCCTGG384010.15902971210573874No Hit
GAACTTTGAAGAGAGAGTTCAAGAGGGCGTGAAACCGTTAAGAGGTAAACGGGTGGGGTCCGCGCAGTCCGCCCG367080.15201850660080357No Hit
CAGAGATGTGTTTAAGTGCTGTGGCCAGAAGCGGGGGGAGGGGGGGGTTT364790.15107015098318388No Hit
GTTAGGGTTCTTTGTTTTTGGGGTTTGGCAGAGATGTGTTTAAGTGCTGT362870.1502750231291097No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC338840.1403235010804628No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC331550.13730450000952496No Hit
CGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCACCGCGGCG330920.13704359868240687No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320020.13252959159417338No Hit
CGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGG302600.125315462834813No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT301460.12484335567145648No Hit
CGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCA300760.12455346530799195No Hit
CCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGC292140.1209836725464715No Hit
CCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTC291390.12067307572847377No Hit
CGACCCGCTCCGGGGACAGTGCCAGGTGGGGAGTTTGACTGGGGCGGTAC288410.11943897103829618No Hit
ATCTGGTTAGGCTGGTGTTAGGGTTCTTTGTTTTTGGGGTTTGGCAGAGA283480.11739731462132451No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT279930.11592715634946864No Hit
CTCCGGGCGGACTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCAC276480.11449841098667912No Hit
GGCGGGGCCGGGGGTGGGGTCGGCGGGGGACCGTCCCCCGACCGGCGACC274820.11381095669617751No Hit
CCTGGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCGTTCCAGTGAGTTCACCCTCTAAATC254080.10522191935581392No Hit
CAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGT252770.10467941024704458No Hit
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAATGAAGGTGAAGGCCGGGGGGG251930.10433154181088712No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)