FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001790264

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001790264
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48179258
Sequences flagged as poor quality0
Sequence length35-76
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA816410816.9452754959406No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN27185355.642542274104761No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTTGCGCCGGTCCA8107161.6827075253006178No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3601930.7476101022560373No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3548460.7365119653772999No Hit
CCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTC3438950.7137822670494428No Hit
CTCCGGGCGGACTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCAC2551520.5295888948725611No Hit
CCTATCTTCTCTATTCTTTTCTTCGCCTTCCCGTACTTCTGTCTTCCAGT2355670.48893862167823343No Hit
CCCTATCTTCTCTATTCTTTTCTTCGCCTTCCCGTACTTCTGTCTTCCAG1831340.38010963141026377No Hit
CGGGCGGACTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCACGCC1660190.3445860457211691No Hit
CCGGGCGGACTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCACGC1635690.3395008698556545No Hit
CCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCGCGGCGCACCGCCG1599080.33190216420518553No Hit
TCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTTGCGCCGGTCCA1591170.3302603788543194No Hit
CCGGCCTTCACCTTCATTGCGCCACGGCGGCTTTCGTGCGAGCCCCCGAC1493600.31000892541765585No Hit
TTCTAATTTCCCTATCTTCTCTATTCTTTTCTTCGCCTTCCCGTACTTCT1431700.29716107292478433No Hit
GCCGGCCTTCACCTTCATTGCGCCACGGCGGCTTTCGTGCGAGCCCCCGA1425810.29593855513507494No Hit
CCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTTGCGCCGGTCCA1407180.2920717458952979No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1384410.28734564571334825No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1235430.2564236252870478No Hit
CTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCT1123190.23312729307703328No Hit
TCCGGGCGGACTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCACG1095600.22740076237786808No Hit
CTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAA1072790.2226663598679747No Hit
CTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCCCCCGGCCGTCCCTCTT1018410.2113793450285183No Hit
CTATCTTCTCTATTCTTTTCTTCGCCTTCCCGTACTTCTGTCTTCCAGTT1004470.2084859837401398No Hit
GGACGGGGCCCCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCC960110.19927870205057951No Hit
GCGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGACCGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCC937480.1945816600164328No Hit
CCCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCAT933460.19374727605809122No Hit
TCCCTATCTTCTCTATTCTTTTCTTCGCCTTCCCGTACTTCTGTCTTCCA901420.18709711137518972No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA860350.17857269615899854No Hit
CCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCGCGGCGCACCGCCGC812640.16867009450415363No Hit
CGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGC808080.16772362911857214No Hit
GGACGGACGGGGCCCCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCG797820.1655940820010138No Hit
CCCCGCTTCGCGCCCCAGCCCGACCGACCCAGCCCTTAGAGCCAATCCTT794720.16495065158537728No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT782970.16251184275191619No Hit
GTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGT778240.161530092472574No Hit
CCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT745340.15470142773888299No Hit
GTCCCGGAGCCGGTCGCGGCGCACCGCCGCGGTGGAAATGCGCCCGGCGG741350.15387327052649918No Hit
GTTCCTTTTACTTTTTTTAACCTTTCCTTATGAGCATGCCTGTGTTGGGT691970.1436240466800049No Hit
CCCAGCCGTCCCGGAGCCGGTCGCGGCGCACCGCCGCGGTGGAAATGCGC688800.1429660871904669No Hit
CTTCTCTATTCTTTTCTTCGCCTTCCCGTACTTCTGTCTTCCAGTTTTCC688160.14283324994336774No Hit
GGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCCCCCGGCCGTCCCTCTTAATC680670.14127863903591042No Hit
CCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCG673790.1398506386295945No Hit
CCCGGAGCCGGTCGCGGCGCACCGCCGCGGTGGAAATGCGCCCGGCGGCG634740.13174549097456004No Hit
CCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT630540.13087374654047185No Hit
GGGCGGACTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCACGCCC626630.13006219398397542No Hit
CCCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT613970.12743450718979527No Hit
CGGCCTTCACCTTCATTGCGCCACGGCGGCTTTCGTGCGAGCCCCCGACT606740.12593386141397195No Hit
TCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGG605950.12576989043708395No Hit
GCCCATTTGAGTATTTTGTTTTCAATTAGGGAGATAGTTGGTATTAGGAT605940.12576781485509803No Hit
TCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCT602230.1249977739383201No Hit
GTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAA597950.12410942484834449No Hit
GCCCCGCTTCGCGCCCCAGCCCGACCGACCCAGCCCTTAGAGCCAATCCT595680.12363826773753968No Hit
GTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCA593380.12316088388077708No Hit
CCCCTATCTTCTCTATTCTTTTCTTCGCCTTCCCGTACTTCTGTCTTCCA581590.12071377271937231No Hit
CGCTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCA568350.11796570217000851No Hit
CTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATGCCAGTATCAGGCGGCGGCTTCGA558630.11594823647969006No Hit
ATTCGGTTCAGTCTAATCCTTTTTGTAGTCACTCATAGGCCAGACTTAGGGCTAGGATGATGATTAATAAGAGGG555350.1152674455883069No Hit
CTAAAAGTATGTTTTTATCTTCTAATTTCCCTATCTTCTCTATTCTTTTC553170.1148149687153754No Hit
GCTTTCGCTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCG551810.1145326895652897No Hit
GCTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAA549100.1139702068471042No Hit
CTTCACCTTCATTGCGCCACGGCGGCTTTCGTGCGAGCCCCCGACTCGCG546540.11343885785870758No Hit
CTTCTAATTTCCCTATCTTCTCTATTCTTTTCTTCGCCTTCCCGTACTTC543720.11285354373867691No Hit
GACGGACGGGGCCCCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGT514760.1068426583074401No Hit
CCCTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAA512810.10643791982018487No Hit
CGGACGGGGCCCCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCC502570.10431252386659837No Hit
GCCCCTATACCCAGGTCGGACGACCGATTTGCACGTCAGGACCGCTACGG485060.10067817980924489No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)