FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001790265

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001790265
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48179258
Sequences flagged as poor quality0
Sequence length35-76
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34927117.249408033639703No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN27022835.608809915669519No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9212131.912053107999297No Hit
CCCCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9192671.9080140254546882No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8945551.8567222434185267No Hit
GCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCG7734941.605450212620543No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4770430.9901418573112936No Hit
CCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGC3533430.7333923656524557No Hit
CCCCCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3355340.6964283260651296No Hit
CGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCA3248300.6742112964877957No Hit
GCGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGACCGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCC3221080.6685615623221097No Hit
GCGGAGCGAGCGCACGGGGTCGGCGGCGACGTCGGCTACCCACCCGACCC3092550.6418841070570244No Hit
CCCCCTCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2418580.5019961079516833No Hit
CCCCTCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2314510.48039552622416887No Hit
GGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAAAACTGGAAGACAGAAGTACGGGAAGGCGAAGAAA2123320.4407124742352819No Hit
AGAACTTTGAAGAGAGAGTTCAAGAGGGCGTGAAACCGTTAAGAGGTAAA2095140.43486348419894716No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2087290.43323415233999657No Hit
TCCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2072670.43019965147657524No Hit
GTTGAAAAGAACTTTGAAGAGAGAGTTCAAGAGGGCGTGAAACCGTTAAG1656300.34377864432864447No Hit
GAACTTTGAAGAGAGAGTTCAAGAGGGCGTGAAACCGTTAAGAGGTAAAC1523590.3162335957934429No Hit
GCTCCGGCGGGTGCGGGGGTGGGCGGGCGGGGCCGGGGGTGGGGTCGGCG1438910.2986575675366358No Hit
GGCGGAGCGAGCGCACGGGGTCGGCGGCGACGTCGGCTACCCACCCGACC1425750.2959261016431594No Hit
AAAGAACTTTGAAGAGAGAGTTCAAGAGGGCGTGAAACCGTTAAGAGGTA1412470.2931697287658519No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1406190.2918662632786914No Hit
GAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAAAACTGGAAGACAGAAGTACGGGAAGGCGAAGAAAAGAAT1315120.27296393813287867No Hit
CGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGG1263350.2622186501917485No Hit
CGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGACCGTCCCCCGACCGGCGA1149290.23854456206029573No Hit
CCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1112530.23091472268003793No Hit
GATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAAAACTGGAAGACAGAAGTACGGGAAGGCGAAGAAA1106350.22963201301273672No Hit
CGAAAATGGAAAGATTAATTGGGAGTGGTAGGATGAAACAATTTGGAGAA1030630.2139157062153178No Hit
TGGAAAGATTAATTGGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAAAACTGGAAGAC984290.20429745929254453No Hit
CCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT966040.20050952216823262No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGG956710.19857300417536525No Hit
GGCGGGGCCGGGGGTGGGGTCGGCGGGGGACCGTCCCCCGACCGGCGACC956100.19844639367422387No Hit
CTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGC910350.18895060608862013No Hit
CCCCCCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT908640.1885956815690271No Hit
GAAAAGAACTTTGAAGAGAGAGTTCAAGAGGGCGTGAAACCGTTAAGAGG885330.18375749995983748No Hit
GAAAGATTAATTGGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAAAACTGGAAGAC866430.17983465000644053No Hit
CCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT851600.17675656192131478No Hit
GCGGGGCCGGGGGTGGGGTCGGCGGGGGACCGTCCCCCGACCGGCGACCG834360.17317825857758123No Hit
GGAAAGATTAATTGGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAAAACTGGAAGAC829740.1722193397000842No Hit
TGAAAAGAACTTTGAAGAGAGAGTTCAAGAGGGCGTGAAACCGTTAAGAG802320.16652809389467974No Hit
CCCCTCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT790830.1641432501928527No Hit
AAAAGAACTTTGAAGAGAGAGTTCAAGAGGGCGTGAAACCGTTAAGAGGT788820.1637260582136819No Hit
AACAATTTGGAGAAGATAGAAGTTTGAAGTGGAAAACTGGAAGACAGAAGTACGGGAAGGCGAAGAAAAGAAT785360.1630079068465521No Hit
CCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTC778820.16165047622775758No Hit
TGGAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAACTATGCCTG752450.15617716653087516No Hit
GGCTCCGGCGGGTGCGGGGGTGGGCGGGCGGGGCCGGGGGTGGGGTCGGC747830.15521824765337813No Hit
CGGCGGGGGACCGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTT724620.1504008218640478No Hit
AAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAAAACTGGAAGACAGAAGTACGGGAAGGCGAAGAAAAGAAT716690.14875488534920983No Hit
GTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAAAACTGGAAGACAGAAGTACGGGAAGGCGAAGA703440.14600473921786009No Hit
GTGTAAATCTCGCGCCGGGCCGTACCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTGGCATGTTGGAAC688380.14287891274705808No Hit
GCGGGGGACCGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCC684920.14216076137992828No Hit
TGTAAATCTCGCGCCGGGCCGTACCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTGGCATGTTGGAACA683830.1419345229434625No Hit
TTGAAAAGAACTTTGAAGAGAGAGTTCAAGAGGGCGTGAAACCGTTAAGA664610.13794525436651597No Hit
ATTTGGAGAAGATAGAAGTTTGAAGTGGAAAACTGGAAGACAGAAGTACGGGAAGGCGAAGAAAAGAATAGAGA638630.13255289236708462No Hit
GGCGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGACCGTCCCCCGACCGGC625350.1297965194897771No Hit
GCCGGGGGTGGGGTCGGCGGGGGACCGTCCCCCGACCGGCGACCGGCCGC609460.12649841971414338No Hit
GGGCGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGACCGTCCCCCGACCGG603590.12528005308840579No Hit
TTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGAC586200.12167061601488342No Hit
GGGCGGGGCCGGGGGTGGGGTCGGCGGGGGACCGTCCCCCGACCGGCGAC579630.12030695865013115No Hit
GGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGC579430.12026544701041264No Hit
CGGCGGAGCGAGCGCACGGGGTCGGCGGCGACGTCGGCTACCCACCCGAC577370.11983787712131223No Hit
GGAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAACTATGCCTGG567370.11776229513538793No Hit
GGGGTGGGCGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGACCGTCCCCCG561110.1164629808121993No Hit
CGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCG534160.11086928736013328No Hit
AGAAGTTTGAAGTGGAAAACTGGAAGACAGAAGTACGGGAAGGCGAAGAAAAGAATAGAGAAGATAGGGAAATT526280.10923372875522493No Hit
GTCGGCGGGGGACCGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCAT525290.10902824613861842No Hit
TCCCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT524950.10895767635109699No Hit
CTCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT517890.1074923154690344No Hit
GGCCGGGGGTGGGGTCGGCGGGGGACCGTCCCCCGACCGGCGACCGGCCG504830.10478160539541725No Hit
CGGAGCGAGCGCACGGGGTCGGCGGCGACGTCGGCTACCCACCCGACCCGTCTTGTAACACGGACCAAGGAGTCT499680.10371268067266623No Hit
GAAGTTTGAAGTGGAAAACTGGAAGACAGAAGTACGGGAAGGCGAAGAAAAGAATAGAGAAGATAGGGAAATT497980.10335983173505911No Hit
GTGGGGTCGGCGGGGGACCGTCCCCCGACCGGCGACCGGCCGCCGCCGGG495610.10286791880439504No Hit
CCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT489760.10165370334262931No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)