FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001793208

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001793208
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2665475
Sequences flagged as poor quality0
Sequence length126
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAGAGGTTGTGCTGGTTGTAGGAGCAGAGGTTGTGCTGGTGGTGGGAA146570.5498832290679897No Hit
GTGGGAACAGGGCTGGGAGTAGTTCCAGGACCAGAGGTTGTGCTGGCTGT78250.29356868850767687No Hit
GTTGTGCTGGTGGTGGGAACAGGGCTGGGAGTAGTTCCAGGACCAGAGGT74940.2811506391918889No Hit
GTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAG74500.2794999015184911No Hit
AGCAGAGGTTGTGCTGGTGGTGGGAACAGGGCTGGGAGTAGTTCCAGGAC71920.26982057606993126No Hit
GCTGGCTGTAGAGGCAGAGGTTGTGCTGGTTGTAGGAGCAGAGGTTGTGC60710.22776428216359185No Hit
GGGAGAGATTGTGCTGGTTGTAGAAGCAGAGGTTGTGCTTGTGGTGGGAA60020.22517562535758168No Hit
GCTGGTTGTAGGAGCAGAGGTTGTGCTGGTGGTGGGAACAGGGCTGGGAG54620.20491657209315414No Hit
CCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGA54360.20394113619523727No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA54310.20375355236871476No Hit
GTCTTCACGAAGATCTGCATCCCACCTCTAAGACGGAGCACCAGGTGCAG46470.17434040836999035No Hit
CTTCTTTTCAATTTCTCCCACGTCAAGAGAAAAGCCAACATGTTTTTCCT45660.1713015503803262No Hit
CTCCAATACATAAAAGGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTT45030.168937994166143No Hit
GTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGAACTTC43260.1622975267072473No Hit
CTTGGATACAGAAAGATGGCTTGTGCTGGTCTTTGAAACAGGGGTTGTGC42620.1598964537277596No Hit
GTGGACTCTTTCTGGATGTTGTAGTCAGACAGGGTGCGTCCATCTTCCAG42190.1582832328196663No Hit
GTAGGAGCAGAGGTTGTGCTGGTGGTGGGAACAGGGCTGGGAGTAGTTCC41420.15539444189122015No Hit
GTCCTTTTTAATTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCT40780.15299336891173243No Hit
GGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTTCAAGACAACCTGAAG40360.15141766476894364No Hit
CTCCAATGCATAAAAGGAACTTCCATAGGGCTGGCAGGAGTCAGGCTGTT37990.14252619139177822No Hit
GTGGGAACAGGGCTGGGAGTAGTTCCAGGACCAGAGGTCATGCTGGTTAT37850.14200095667751528No Hit
GTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGCACTGC37070.13907464898376462No Hit
CACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGCAC35930.13479773773905213No Hit
CTCGGCTCCACCTCGAGAGTGATGGTCTTACCAGTCAGGGTCTTCACGAA35510.13322203359626333No Hit
CTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTT35420.13288438270852287No Hit
CCAGTGAGTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCCTG34890.13089599414738462No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGG34560.12965794089233626No Hit
GTGCTGGTGGTGGGAACAGGGCTGGGAGTAGTTCCAGGACCAGAGGTTGT34550.12962042412703179No Hit
AGCAGAGGTTGTGCTGGTGGTGGGAACAGGGCTGGGAGTACTTCCAGGAC34110.12796968645363396No Hit
GTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTG33030.12391787580074845No Hit
GGCAGAGTTTGTGCTGGTTGTAGGTGCAGAGGTTATGCTGGTGGTGGGAA32860.12328009079057203No Hit
GCAGAGGTTGTGCTGGTGGTGGGAACAGGGCTGGGAGTAGTTCCAGGACC32810.12309250696404955No Hit
GGCAGAGGTTGTGCTGGTTGTTGGGGCCGAGATTGTGCTGGTTGTAGGGG32310.12121666869882479No Hit
GTCCACTTTGGGTCCTGAGACATCAATGTCAGCCTTGGGCAGGTTCACAT32270.1210666016376068No Hit
GTGGGAACAGGGCTGGGAGTAGTTCCAGGACCAGAGGTTGTGCTAGTTGT31350.11761505922959323No Hit
CTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAA31040.11645203950515386No Hit
GCCAACATGTTTTTCCTCCAATGCATAAAAGGAACTTCCATAGGGCTGGC30940.11607687185210891No Hit
GCTGGCAGGAGTCAGGCTGTTCAAGACAACTGGAAGGAGTTGAATAACAT30440.11420103358688413No Hit
GCTGGTTGTAGAAGCAGAGGTTGTGCTTGTGGTGGGAACAGGGCTGGGAG30350.11386338269914367No Hit
GGCAGAGGTTGTGCTGCTTATAGGGGCAGAGGTTGTGCTGGTTGTAGGAG29720.11149982648496046No Hit
GTACTTTTCAATTTCATCCATGTCAACAGCCAAGCCAACACGCTGTTGCT28780.1079732505463379No Hit
CCCTGCTGAGCCTGGGGCATGGTGGGCCTTGGTCTTCTTCCTCTTCTTGG28640.10744801583207496No Hit
CTGGTGGTGGGAACAGGGCTGGGAGTAGTTCCAGGACCAGAGGTTGTGCT28460.10677271405659405No Hit
GTCAGACAGGGTGCGTCCATCTTCCAGCTGTTTCCCAGCAAAGATCAACC28250.10598486198519963No Hit
GTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCT28170.10568472786276367No Hit
GCTGGGAGTAGTTCCAGGACCAGAGGTTGTGCTGGCTGTAGAGGCAGAGG28120.10549714403624119No Hit
CCAGTGAGTCCTGCAAGACTTCAGGCTCTACTACCTCCAGCAGCTCCCTG27130.10178298427109614No Hit
CTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAA27080.10159540044457366No Hit
GTCTTCACGAAGATCTGCATCCCACCTCTGAGACGGAGCACCAGGTGCAG26890.10088258190378825No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)