FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001793209

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001793209
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2665475
Sequences flagged as poor quality0
Sequence length126
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTGGTCCTGGAACTACTCCCAGCCCTGTTCCCACCACCAGCACAACCT120140.4507264183682083No Hit
CAACCTCTGGTCCTGGAACTACTCCCAGCCCTGTTCCCACCACCAGCACA75870.284639698365207No Hit
CATCACTCTCGAGGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAA58390.21906039261294893No Hit
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC55150.2069049606542924No Hit
GGAACTACTCCCAGCCCTGTTCCCACCACCAGCACAACCTCTGCTCCTAC47180.17700409870660952No Hit
CAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATC42890.16090940639098097No Hit
GGCAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGA42170.1582081992890573No Hit
CTCTGGTCCTGGAAGTACTCCCAGCCCTGTTCCCACCACCAGCACAACCT40880.1533685365647774No Hit
GTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTAC40740.15284330185051445No Hit
CTCCCAGCCCTGTTCCCACCACCAGCACAACCTCTGCTCCTACAACCAGC40100.15044222887102673No Hit
GGGGAAGAAAAGAAGGGGAAGAAGATCAAAACCCACCATGCCCCAGGCTC36170.13569814010636003No Hit
CAACCTCTGGTCCTGGAAGTACTCCCAGCCCTGTTCCCACCACCAGCACA35920.13476022097374762No Hit
CAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTC35770.1341974694941802No Hit
CTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTATGTATT34890.13089599414738462No Hit
CCTCTGGTCCTGGAACTACTCCCAGCCCTGTTCCCACCACCAGCACAACC34820.13063337679025316No Hit
CAACCTCTGGTCCTGGAACTTCTCTCAGCCCTGTTCCCACCACGAGCACA34280.12860747146381038No Hit
CCAAGACCCATCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAG32830.12316754049465856No Hit
CTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTGCTCCGTCTTAGAG32680.12260478901509111No Hit
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA31300.11742747540307076No Hit
GGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGAC31060.11652707303576285No Hit
CTTCAAGCCCTGTTCCCACCACCAGCACAACCTCTGCCCCTACAACCAGC31020.11637700597454488No Hit
GCCAGCACAACCTCTGGTCCTGGAACTACTCCCAGCCCTGTTCCCACCAC30500.11442613417871111No Hit
CAATCTCTCCCCTTACAACCAGCACAACCTCTGCTCCTATAACCAGCATG30170.11318808092366277No Hit
CCAGCACAACCTCTGCTCCTATAACCAGCATGACCTCTGGTCCTGGAACT29380.11022425646460762No Hit
GGATGTGTCTGTGCCCAAGGTAGAAGGTGAAATGAAAGTGCCAGATGTTG29080.10909875350547275No Hit
CCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGGGCCTGAAGTCTTGC28450.10673519729128955No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG27950.10485935902606476No Hit
CTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTACTCATT27850.10448419137301981No Hit
CCCTGTCTGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTGCTC27730.10403399018936586No Hit
CACCAGCACAACCTCTGCTCCTACAACCAGCACAACCTCTGCCTCTACAG27510.10320862135266697No Hit
CTCCCAGCCCTGTTCCCACCACCAGCACAACCTCTGCTCCTACAACCAGA27110.10170795074048715No Hit
CTCTGCTCCTACAACCAGCACAACCTCTGCCTCTACAGCCAGCACAACCT27030.10140781661805119No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)