FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001793218

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001793218
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1547021
Sequences flagged as poor quality0
Sequence length126
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAG56680.3663815811162227No Hit
CCTCCATTCACCAGCCTCAGGGCCAAACTGGATTCAGGTCCTGCTGTTGA50110.3239128622041976No Hit
CTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAA38180.24679690838068777No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGG37800.24434057456233627No Hit
CTCCAATACATAAAAGGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTT34880.22546558837921396No Hit
CCTCCATTCACCAGCCTCAGGGCCAAACTGGATTCAGGTCCTGAGCAGAT34220.2211993243789192No Hit
GTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTC33220.21473528801483627No Hit
GGTGTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACG33180.21447672656027292No Hit
CTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAA32800.21202039274192142No Hit
GGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCT32040.20710772510521835No Hit
CCTCCATTCACCAGCCTCAGGGCCAAACTGGATTCAGATCCTGCTGTTGA30990.20032048692293125No Hit
GTCTTCACGAAGATCTGCATCCCACCTCTAAGACGGAGCACCAGGTGCAG30660.19818735492278383No Hit
GTCCTTTTTAATTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCT29590.1912708360132151No Hit
CTGCTGTTGATGCATGTGAGGTTGGCCAAGTGTCTGGGCTGGGTGTCGGC29290.18933162510399018No Hit
GTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTG27590.17834276328504914No Hit
GTCAGGGTCTTCACGAAGATCTGCATCCCACCTCTGAGACGGAGCACCAG27480.17763171928500002No Hit
CCTCCATTCACCAGCCTCAGGGCCAAACTGGATTCAGATCCTACTGTCGA25540.16509148873867904No Hit
CCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGA23940.1547490305561463No Hit
ATCTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGG23440.1515170123741048No Hit
CTCGGCTCCACTTCGAGAGTGATGGTCTTACCAGTCAGGGTCTTCACGAA22270.14395408982812774No Hit
GTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCT22100.1428552036462336No Hit
GTCTTCACGAAGATCTGCATCCCACCTCTGAGACGGAGCACCAGGTGCAG21530.13917070291870634No Hit
GTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCCTGCTGAGCC20300.1312199381908843No Hit
CACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGCAC19980.1291514465543777No Hit
GGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTT19950.12895752546345524No Hit
CTTCTTTTCAATTTCTCCCACGTCAAGAGAAAAGCCAACATGTTTTTCCT19610.12675975309966703No Hit
GGGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCAT19320.12488518255408297No Hit
CCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCA19240.12436805964495634No Hit
CCTTGACATTCTCGATGGTGTCACTGGGCTCCACCTCGAGGGTGATGGTC18010.11641729491713429No Hit
CTCCAATGCATAAAAGGAACTTCCATAGGGCTGGCAGGAGTCAGGCTGTT17890.11564161055344434No Hit
CCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTTCTCCCACGTCAAGA17410.1125388730986845No Hit
CTTGGATCTTTGCCTTGACATTCTCAATGGTGTCACTCGGCTCCACTTCG16260.10510523127998908No Hit
CTCCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAG16080.10394170473445416No Hit
GTCCCAGTAGTCATCACACACGGTGCCCCAGGAGCCTCGGTATAGGACCT15940.10303673964348255No Hit
GGACTCTTTCTGGATGTTGTAGTCAGACAGGGTGCGTCCATCTTCCAGCT15780.10200249382522927No Hit
GTGGGTTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTC15610.10090360764333517No Hit
CCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTC15600.10083896727969432No Hit
GTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTATCATCC15510.10025720400692686No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)