FastQCFastQC Report
Fri 24 Mar 2023
EGAF00001794350

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001794350
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65325398
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1346610.2061388129621499Clontech SMART CDS Primer II A (100% over 26bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC1192610.1825645210764732TruSeq Adapter, Index 12 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA777550.11902721205005135No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)