FastQCFastQC Report
Fri 24 Mar 2023
EGAF00001794364

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001794364
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60171280
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT994810.1653297054674589Clontech SMART CDS Primer II A (100% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA631000.10486730546533163No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)