FastQCFastQC Report
Sun 14 May 2023
EGAF00001794987

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001794987
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24801683
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTGGTCAATCTCGTA11001414.435751396387092TruSeq Adapter, Index 25 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTGGTCAATCTCGTAT2047830.8256818700569635TruSeq Adapter, Index 25 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTGGTCAATATCGTA393590.15869487566630053TruSeq Adapter, Index 25 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)