FastQCFastQC Report
Tue 5 Sep 2023
EGAF00001794997

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001794997
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37030546
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAAGCTAATCTCGTA8863632.393599597478255TruSeq Adapter, Index 5 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAAGCTAATCTCGTAT4125641.1141180581026269TruSeq Adapter, Index 5 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)