FastQCFastQC Report
Sun 2 Apr 2023
EGAF00001795007

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001795007
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17327561
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACAACCAATCTCGTA4571092.6380458276845773TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACAACCAATCTCGTAT3244711.872571679303279TruSeq Adapter, Index 1 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACAACCAATATCGTA173420.10008332967346067TruSeq Adapter, Index 1 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)