FastQCFastQC Report
Thu 9 Feb 2023
EGAF00001795035

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001795035
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81587532
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAGTCAAATCTCGTA11963741.4663686603487405TruSeq Adapter, Index 5 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAGTCAAATCTCGTAT3597080.4408859922371472TruSeq Adapter, Index 5 (97% over 38bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)