FastQCFastQC Report
Mon 3 Apr 2023
EGAF00001795039

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001795039
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40109994
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGTATCAATCTCGTA9464682.359681230568122TruSeq Adapter, Index 8 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACGTATCAATCTCGTAT3733860.9309051504719745TruSeq Adapter, Index 8 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGTATCAATATCGTA425160.10599851996986089TruSeq Adapter, Index 8 (97% over 37bp)

[WARN]Adapter Content

Adapter graph