FastQCFastQC Report
Tue 27 Sep 2022
EGAF00001795083

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001795083
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11469676
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTA5235564.564697381164037TruSeq Adapter, Index 22 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTAT2382152.076911326876191TruSeq Adapter, Index 22 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATATCGTA121160.10563506763399419TruSeq Adapter, Index 22 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)