FastQCFastQC Report
Tue 26 Sep 2023
EGAF00001795087

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001795087
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27446234
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTGAGAATCTCGTA8726383.179445311149063TruSeq Adapter, Index 16 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTGAGAATCTCGTAT1936530.705572210744833TruSeq Adapter, Index 16 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTGAGAATATCGTA276310.10067319254073255TruSeq Adapter, Index 16 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)