FastQCFastQC Report
Tue 14 Feb 2023
EGAF00001795227

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001795227
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52550921
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAAGCTAATCTCGTA29775285.665986329716277TruSeq Adapter, Index 5 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAAGCTAATCTCGTAT28739235.4688346946383675TruSeq Adapter, Index 5 (97% over 36bp)
AGCACACGTCTGAACTCCAGTCACACAAGCTAATCTCGTATGCCGTCTTC4099590.7801176310497012TruSeq Adapter, Index 5 (96% over 27bp)
AAGAGCACACGTCTGAACTCCAGTCACACAAGCTAATCTCGTATGCCGTC2749220.5231535333129557TruSeq Adapter, Index 5 (96% over 30bp)
CACACGTCTGAACTCCAGTCACACAAGCTAATCTCGTATGCCGTCTTCTG2072960.39446692095082403Illumina PCR Primer Index 10 (96% over 25bp)
GCACACGTCTGAACTCCAGTCACACAAGCTAATCTCGTATGCCGTCTTCT1791600.3409264701564412TruSeq Adapter, Index 5 (96% over 26bp)
AGAGCACACGTCTGAACTCCAGTCACACAAGCTAATCTCGTATGCCGTCT1732280.3296383711334003TruSeq Adapter, Index 5 (96% over 29bp)
GAGCACACGTCTGAACTCCAGTCACACAAGCTAATCTCGTATGCCGTCTT923900.17581042965926325TruSeq Adapter, Index 5 (96% over 28bp)
CACGTCTGAACTCCAGTCACACAAGCTAATCTCGTATGCCGTCTTCTGCT542650.10326174873319537Illumina PCR Primer Index 10 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)