FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001795558

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001795558
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences864251
Sequences flagged as poor quality0
Sequence length32-301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAACTTGAGGCTCGTGGGCGCTCAGCCTTCTCTTGTGTCTTTTTAGA158231.8308338665503423No Hit
TGTTGGGTAGTTCCTTATGTAATGTTTTTTGTTTTTTATTAATAGGATCG151771.756087062670451No Hit
ACCGGCATTGATTTTGGGACCCACATGCCCGAGGAATACACTCACAGGCT147321.7045973912671202No Hit
GGATTACGTTTGTGATTTTAAGCAGATTTACTTATTAGGCTCTATAACTA145271.6808774302835634No Hit
TTGCTATTCTCAGATGACTCTGTGTTTTTATAATAAAATAAACTGTACTT136271.5767410162094115No Hit
GCCTCCCAAAGCATTATGAATATGGGCATGAGCCACTTCACCCAACCAAA125941.4572155542776346No Hit
TGCCAAACAACAAAGTGCTCAATCTACTATATGTACAAGAAGATTACCAA125161.4481903983912081No Hit
GTATCTTTGACAATTACCTGATGAAATTAAAGTTTTTTTGTATTCTATAA120311.3920724419179151No Hit
CATCTTTATTGCCCCTATATCTGTCATATTTTTATATAAAAATGTGTATA119381.3813116791302527No Hit
CATATGATAACAGCAAATACATGTTACTTAAAATTACCATTTCTCTCATT115201.3329461001491465No Hit
CATCAAATGCTCTTTAATGGCCTTTTAAAATTAAAAGGTATTTAATCTGT114041.3195240734462557No Hit
AGGCAAACAACATTCCATGATGACCAAATATTTACTAAAAAGAATAGATA108631.2569265178750155No Hit
TCCCTCATCCTCTCCCCATAGAAAAGTCCAACCTGATCGTGGTCCAGAGA106671.2342479210322002No Hit
TGGGAGCTAGCAGTGTAAACAGAGTACATACATAAAAATTACATTTTAAT105821.2244128152585303No Hit
AAGAGATGGAATCAGTGATTTCAGATTGTTTGTTTCTTTTTTCTCCAGTT103371.1960645692050111No Hit
ATTTCCCAGGGCCTCTTACCCACTCCAGGCTGTCAGCAGGGATCTTGTGA99471.1509387897728784No Hit
CCTGGTCTTTGAGGGGAACAACTTATATCTTACGTTTCATCTTATGTTTC99351.1495503042518898No Hit
AGAAGGAGAGTGTCTGTCGCTCAGTCCCACTCAGGGGCCACTCTTCTTTG96441.115879530367914No Hit
ACATAGAAAGCCAGGGTCCCAGATCAGCCACTCATTCCAGGGGAGGAAGG96181.1128711450724385No Hit
CAGGAAATTTCTAAATGTGACATGACCTACTTACTGTACCTGGTGACAGA92591.0713322865695267No Hit
AGATACGTTGACAACATTGGTGTGTAACAAAATCCGTATTTATAATGTGT86380.9994781608583617No Hit
GATACTGGTTCTACTGTTTCTAAGTATGTGATTAAAATGTACATTGTTCT85680.9913786619859277No Hit
GTACACCACACACACACACGCACACGCACACGCAGAGCTGAGGGAAGGCT83180.9624518802986632No Hit
AAAGGTTTCAGTGAGGTGAACAGTTTAAAGGCCTTGGGAATAAGAAAATC82300.9522696531447461No Hit
AAACAGCAGCATGCTAATGAACTTAAATTACCTGTGAAGAATTGGAGGCA76080.8802998203068322No Hit
AGTGCAGTTCAGTCTCCCAACCCCGCCTCTCCCTGCTGCCAACCAGGCAC74970.8674563292376867No Hit
ATGAGGCCTCTTATACTGCCAAATCAATATAAAGAAGTAAAAGAAATCCC70740.8185122146228352No Hit
CCTCCAATAGCTTGCTTTTCACAATTGTCCTTTGTTTTGTTATAGTCCTG68600.7937508894985368No Hit
AGGGGAGGAGTTCAGAGGAAGACACTTGCCAGGGAAATATCCCTGTGGCT68300.7902796756960653No Hit
GCCAATATTTAACCAATTTTGACCTACATAAAAATACTATTTCAAATGGT68120.788196947414582No Hit
CTAAAATGCCCTCCCCTCGAACCCTTACCAAACTCCTATGCATACTACAG68060.7875027046540877No Hit
GAGATACACAGTCTACCTGGTAAGAAAAAAAATAAAGTCTTCCTTATAAA67450.7804445699223952No Hit
ACTATTCCTGCTTGACCTTCAATGCTGTTCCTCAGTTTGTCACTAAAATC64580.7472366245454156No Hit
TTGTTTGTTTGTTTGCTTGCTTGTTTTAAGATTGTTCCAGGACACGAAGG64120.741914096714959No Hit
GGTGAGCAGTATTTTAAGAAGGTCCTGTTGTCAGTTTTTCAGATTTTCTT58590.67792805562273No Hit
CATCACTAAAACTCTAAGGGCTAAGCCAGAGAAGGGAAGGCTCACCTTAA54960.6359263686128219No Hit
TCACTTCAGGGCACAGAGAGGTCACCCAGCCTTCTGCTTCTTCCCTCAGG54460.630141012275369No Hit
TAAGTGCAAATAGTGTATCTGACCTATTATCAATCATGTTTATACTTTTA54280.6280582839938861No Hit
TATGTAAGACATTCTACTGCCATCTGCAGCATTCCAAATACTTCATGAAT52330.6054953942778197No Hit
CTAAAGGCTGAATGAAAGGGTAATTCATATACTGAAGATCACACCCAAGC51790.5992472094333706No Hit
CTCCCAAATTGCTGAGATTACAGATGTGAGCCACTGTGCCCAGCCTGATT50550.5848995257164874No Hit
GGAAGCTTCTAATAAAATACTCATCCATATATACCTTTTTCACAAGGTGA48480.5609481504794325No Hit
TACTACCCAGCTAAAATTATCATCTTTGTAATTAAAGCTATAGCTTAAAA47280.5470632952695456No Hit
AAAAACTCACGCGACAGTAATCTGTTAAGCCATTTATTTATAATTTATAA46570.5388480892703624No Hit
GTATTCTTTACATGGCTTTTGGTCTTCTAAGTGAAGCTTTTTGTTTTTCT46130.533756975693404No Hit
ACCCACCCCTTTGAAAGAGAACACACTTACTCTCCACGTCGCGGACACAG45830.5302857618909321No Hit
AAATGACTTAGTTCTGGTTGAGATGAAAGGATTCCACTGAAAGTTTTCTG45820.5301700547641831No Hit
CAAACCACGCTCCTTCCACTGGGCCCAATATGACATCACAGCCCTCCCCA45290.524037577046483No Hit
CCCCCACTTTGACAGAAGGGCAGACTGACATCCAGTCTGGGGAGATTGGG44940.519987827610266No Hit
CTATTTCATATTTAACCACAGTTCTTTTCCCGTAGGCTGATCCTTATTCA44510.5150124211600565No Hit
GCTGGCCTGAGACATTCCTATGTCCTGCTCGCCCACAGGCCTTCTTCGAG44400.513739642765817No Hit
GATTCTGGCCTCTCTCCCTCTCAGGGCAGGGTGCCATTTGCCGTGCCTGA44300.5125825714983263No Hit
CATTTTCCTGTGTCTGGCCCCCTTAGGAGGACAAAGGCCTGCCCCATGGC42460.49129246017649963No Hit
CAGCACTCTTTAGATAAACAGGTCATAAACAAGGAATTATATCAGACTAA42240.48874690338802035No Hit
GGTGAGCACTGAGGGAATGAAAGTGGGATCAGGGATCCACTTCCTTGCCC42200.48828407488102415No Hit
TCTTGTAGGAGTTAGGCCTTGAAAGAGAGATTTAATTGTTTTATTTGTTT42140.48758983212052975No Hit
GCTCAAAAAAAAAAAAAAGAAAAAAGTGGATTTATTTTTATTTTACAGGT41940.4852756895855487No Hit
CTGAGAGCATCTGGTTGGGAAGGACTGATCTAACAGCGGCTGGCAGCTGG41650.481920182909826No Hit
GTCAAAAAATCTGGAGTTCAGTTGGGATTTTATAATTGATTGTTAAACAT41270.47752331209336174No Hit
GAATGTTGTTTCTAGGTCCTACTCTAAATAATATTAACAAGCATTTAAAT40380.46722537781269563No Hit
GTGTATTTATTGTAGCCGAGTATCTAATTAAACAAGTTTTTACTAAATCT39890.4615557286019918No Hit
GGAAGAACAGGATAGAAAGACTGCTTATATATTGGTCTAAATATGTTATT37580.4348273823229594No Hit
GTGAGGGGACTTGCTAAGTATTGTTAACTAATTTTAAATTAAACAAATTT37230.43077763288674237No Hit
AAGGTACAATGATTTCCACTTCTCTTATTTACATTTTCTAATCCCTTTCT36140.4181655560710951No Hit
CAGGAGCTTCCAAATAGTATGTTCTCATTAAAAGAGGTGTTCTTGTGACA35920.41561999928261584No Hit
CACATGGCATCTGTACAGTGTCTATAACAAAATAACCCAAAGTATGAGAT35560.41145454271964976No Hit
CCTATTTCTCCTTCCTAACAGTTTACCAAAGTTGAATCATATATAAGCTT35480.41052888570565726No Hit
GGAATGTTCTATTATTAAACTCATCATGTATACTTACAGGGCTTTTGGAA33210.38426336793362115No Hit
AACAGGTAGAAATAGCCCATGTCATTTAAAATTAGCACCCTGAGAAGCTC29270.33867475999449237No Hit
CATATAAGGCAAAGCATTAGGTACTTGGTTTATATATTAAAGATCTTACT28370.3282611185870771No Hit
CCAATACGTGTTAAAAGCAAGTTACATTTTCTCTTTTAGGAAATTAACCA28100.32513702616485257No Hit
GTTTTATGTATGATCTCTTACCTATGACTCTACTGAAATAGAATTTCTAT27360.3165746987854223No Hit
GGATTCCATCTTAAATCCATCTTTCTCTAGAACTGAGTTTACAGTATTAA26540.3070867143919995No Hit
AGCAGTATGTTGAGTTTATGGCAGATTAATCTATCATCTTTTAGAAATTT26420.30569822887101084No Hit
CAAGTTACACTCTAGATCCTAAACGTAAGAAGCAACACTCATTACATTAC25780.2982929727590712No Hit
TTTTCATTTTGGAAGTTCACTGGTCTATGAACAAAACTTTTTAAAACGAT25380.2936646876891088No Hit
TGGTGAAGTTTCATTTCATGAGGAAAAATTCACAAACAACAACCTTCAAA24600.2846395318026823No Hit
CTAAGTTTCAATATATAAATACGAATAAATCGAATAAATAGCCTTAAAAC22750.2632337133541066No Hit
GCAGTTTTAAAATCCTTTTTCTGTATGGGATTATGGAATATTTAAGTTAA22070.25536562873517066No Hit
ATATTTCAACAAAAAAATTATGCCTATTAGAATCAAAATATGATAGCAAA21970.25420855746768006No Hit
CCTTATAGCATAGTGGGAGACAGACACATAAACAAGAAGGAAGAAGGTGT21210.24541481583475172No Hit
CAGCCTTGAACCGATTTTAGATGGCATCTTAATAACACAAGATTTTAAAA21000.24298496617302148No Hit
CTAGTTCTGTTATGATGGATCAATGTTTTATTTTAAGGTAGAATTTTTAG20340.23534829580758365No Hit
TAAATAGTTGCCATTCCAAGTGTCTTATTTTTGTTCAAATTTATGTTTTT20330.2352325886808346No Hit
CCCTATATCTGTCATATTTTTATATAAAAATGTGTATATTAGTTTAATTG20290.23476976017383835No Hit
CAGTGGAGGTTAACATTCATCAAGATTAATAACTGGTGTACTTGATAGGC20100.2325713247656063No Hit
ACAGTCATGACCCACAGCAAACAGAACTGTTTTAGATATGCTGGGTATTC19320.22354616887917977No Hit
GTGGTGGTATGTTCTAAGCTTTCTAATTTTTTTAATGTGACTATTTAGAA19130.2213477334709477No Hit
GTTTTCTGTTGATATCTTTGATTACTTAACTTAAAAACAAAATAACTCCT17890.20700004975406452No Hit
CATGGTAGTAGTATCAGTAGTAAAAGTATTTATTCCCATATGTCATTTTC16940.196007872712904No Hit
GAAGTATCATTCTCCATGAATGTCATATTGAGATTACATATAAGGTATCA16490.1908010520091964No Hit
TCTACCCACTGCAGTATCTAGACAGTAATACACATTTTAATGTTAAGCAA15390.17807326806680004No Hit
GTCCTTTGGTGAAGCTATTTATACATGTATATCTTAGGGTTCTGTTTTTA15230.1762219540388151No Hit
CAGTAAAACACTAATCCAGCCAATAAAAAAAACAAACCGCTGCACAGCTG14770.17089942620835846No Hit
ACGCATCTTCTCTCCCTTCCTGTGTCTTCTACTTTTTGTGCTATTTTCAC14360.1661554340116471No Hit
GTGTATTACCTTAATTTGAGTGATTCTTTAGATGTATTTAGTATTTGTAA14100.16314704871617158No Hit
GAATTTGTTCTTACAAAAGATAGAGTATACTAAATTATTTATGAAATATA12170.14081557325360342No Hit
AAGACCAAGTCACTCTTTCTATGCAAAAACACTCACTCAGTTAACTGGTG11560.13375743852191088No Hit
ATCAAGCTGAGTGACCCTGGGGTCAGCCCCGCTGTGTTAAGCCTGGAGAG11540.13352602426841276No Hit
CCTCTTCTTTATTTTCAGAGTGTCTTTTCTTTTTTGCTACTAGAGTAAAA11470.13271607438116936No Hit
GAATACATTTCATTCAAATTTATCCGAAACTTTATTAGTATAAATTCTCC11450.13248466012767124No Hit
CATTCAAGATAGAGAAAACACTGTCTGCCAAGAATAATTGTTTTTATTTC11080.12820349643795612No Hit
GTTTGTTTGTTTGCTTGCTTGTTTTAAGATTGTTCCAGGACACGAAGGGA10710.12392233274824097No Hit
GTCACTATTGGTAACAGAAAAGCTGCACTTTAGGATAACAAAGTCATACG10640.12311238286099756No Hit
TTCAAGCACAGGGTAGAATATTGGGCTGAGTAACACTTGCCTTCATCATG10280.11894692629803147No Hit
TTGTTTGTTTGTTTGCTTGCTTGTTTAAGATTGTTCCAGGACACGAAGGG10240.11848409779103525No Hit
CCCAATAGACCCACCCCAATCTCCCCAGACTGGATGTCAGTCTGCCCTTC10220.11825268353753712No Hit
ATCATGTGTGATTTTGTAGTTCTGTTAAAGTTCATGGCTTTTGTGTTTTA9780.11316156996057858No Hit
GACTTCTGAATGAATTTATTTCAGAGTAATTTTCCAGAACTTACTGGTTG9600.11107884167909554No Hit
CATTCAAGATAGAGAAAACACTGTCTGCCGAGAATAATTGTTTTTATTTC9590.11096313455234649No Hit
CACTCAAATCCTTCTAACAATACTTTATATATTTTAAATACCATTTTGAA9360.10830187063711814No Hit
ATCTTTGACAATTACCTGATGAAATTAAAGTTTTTTTGTATTCTATAATA9260.10714479936962758No Hit
AACCACAGTTCTTTTCCCGTAGGCTGATCCTTATTCAAAATGGGCCATTC9010.10425212120090113No Hit
CTCAAGTTTTCAGAAAAGAAGCCATGACATTAAATGTTAAAATACAGTAA8950.10355787844040679No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)