FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001795566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001795566
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences893111
Sequences flagged as poor quality0
Sequence length32-301
%GC39

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAACTTGAGGCTCGTGGGCGCTCAGCCTTCTCTTGTGTCTTTTTAGA274873.0776689571621No Hit
ACCGGCATTGATTTTGGGACCCACATGCCCGAGGAATACACTCACAGGCT261542.9284153929354804No Hit
AGGCAAACAACATTCCATGATGACCAAATATTTACTAAAAAGAATAGATA255312.8586592260088612No Hit
AAGAGATGGAATCAGTGATTTCAGATTGTTTGTTTCTTTTTTCTCCAGTT241012.698544749756749No Hit
TGTTGGGTAGTTCCTTATGTAATGTTTTTTGTTTTTTATTAATAGGATCG209042.340582525576328No Hit
AAACAGCAGCATGCTAATGAACTTAAATTACCTGTGAAGAATTGGAGGCA197602.212490944574639No Hit
GGATTACGTTTGTGATTTTAAGCAGATTTACTTATTAGGCTCTATAACTA189822.1253797120402727No Hit
CCTCCAATAGCTTGCTTTTCACAATTGTCCTTTGTTTTGTTATAGTCCTG183872.058758653739569No Hit
GTATCTTTGACAATTACCTGATGAAATTAAAGTTTTTTTGTATTCTATAA139871.5660987268099933No Hit
CATCAAATGCTCTTTAATGGCCTTTTAAAATTAAAAGGTATTTAATCTGT131691.4745087676671769No Hit
TCCCTCATCCTCTCCCCATAGAAAAGTCCAACCTGATCGTGGTCCAGAGA108771.2178777330029527No Hit
ATTTCCCAGGGCCTCTTACCCACTCCAGGCTGTCAGCAGGGATCTTGTGA101631.1379324630421077No Hit
TAAGTGCAAATAGTGTATCTGACCTATTATCAATCATGTTTATACTTTTA100741.1279672963383052No Hit
TTGCTATTCTCAGATGACTCTGTGTTTTTATAATAAAATAAACTGTACTT97621.0930332287923898No Hit
CCTGGTCTTTGAGGGGAACAACTTATATCTTACGTTTCATCTTATGTTTC96221.0773576856628124No Hit
CATCACTAAAACTCTAAGGGCTAAGCCAGAGAAGGGAAGGCTCACCTTAA95411.0682882642806997No Hit
GCCTCCCAAAGCATTATGAATATGGGCATGAGCCACTTCACCCAACCAAA88450.9903584212936578No Hit
TGCCAAACAACAAAGTGCTCAATCTACTATATGTACAAGAAGATTACCAA85100.9528490859478832No Hit
GCCAATATTTAACCAATTTTGACCTACATAAAAATACTATTTCAAATGGT84220.9429958874092919No Hit
CATCTTTATTGCCCCTATATCTGTCATATTTTTATATAAAAATGTGTATA82510.9238493311581651No Hit
AGATACGTTGACAACATTGGTGTGTAACAAAATCCGTATTTATAATGTGT81940.917467145741123No Hit
GTACACCACACACACACACGCACACGCACACGCAGAGCTGAGGGAAGGCT79940.8950735126988695No Hit
CAGCCTTGAACCGATTTTAGATGGCATCTTAATAACACAAGATTTTAAAA78550.8795099377345033No Hit
ACTATTCCTGCTTGACCTTCAATGCTGTTCCTCAGTTTGTCACTAAAATC77040.862602744787602No Hit
CACATGGCATCTGTACAGTGTCTATAACAAAATAACCCAAAGTATGAGAT70610.7906072145567572No Hit
CAGGAGCTTCCAAATAGTATGTTCTCATTAAAAGAGGTGTTCTTGTGACA70030.7841130609745037No Hit
CATATGATAACAGCAAATACATGTTACTTAAAATTACCATTTCTCTCATT67170.7520901657240813No Hit
ATGAGGCCTCTTATACTGCCAAATCAATATAAAGAAGTAAAAGAAATCCC65270.7308162143339405No Hit
AGGGGAGGAGTTCAGAGGAAGACACTTGCCAGGGAAATATCCCTGTGGCT62370.698345446422673No Hit
GAGATACACAGTCTACCTGGTAAGAAAAAAAATAAAGTCTTCCTTATAAA61780.6917393246752083No Hit
TGGGAGCTAGCAGTGTAAACAGAGTACATACATAAAAATTACATTTTAAT57880.6480717402428141No Hit
TCTACCCACTGCAGTATCTAGACAGTAATACACATTTTAATGTTAAGCAA55600.6225429985746452No Hit
ACCCACCCCTTTGAAAGAGAACACACTTACTCTCCACGTCGCGGACACAG54770.6132496408621101No Hit
GCTGGCCTGAGACATTCCTATGTCCTGCTCGCCCACAGGCCTTCTTCGAG54610.6114581502187297No Hit
TCACTTCAGGGCACAGAGAGGTCACCCAGCCTTCTGCTTCTTCCCTCAGG54340.6084350097580256No Hit
AGTGCAGTTCAGTCTCCCAACCCCGCCTCTCCCTGCTGCCAACCAGGCAC53850.6029485696626735No Hit
CAGGAAATTTCTAAATGTGACATGACCTACTTACTGTACCTGGTGACAGA50040.5602886987171808No Hit
CTCCCAAATTGCTGAGATTACAGATGTGAGCCACTGTGCCCAGCCTGATT49560.5549142267870399No Hit
CTAAAATGCCCTCCCCTCGAACCCTTACCAAACTCCTATGCATACTACAG48150.5391267154922512No Hit
GATACTGGTTCTACTGTTTCTAAGTATGTGATTAAAATGTACATTGTTCT47810.5353197978750681No Hit
TTGTTTGTTTGTTTGCTTGCTTGTTTTAAGATTGTTCCAGGACACGAAGG46270.5180767004325331No Hit
GAATGTTGTTTCTAGGTCCTACTCTAAATAATATTAACAAGCATTTAAAT45330.5075516929026739No Hit
AAAGGTTTCAGTGAGGTGAACAGTTTAAAGGCCTTGGGAATAAGAAAATC45240.5065439794157726No Hit
GGTGAGCAGTATTTTAAGAAGGTCCTGTTGTCAGTTTTTCAGATTTTCTT45000.5038567434507022No Hit
CATTTTCCTGTGTCTGGCCCCCTTAGGAGGACAAAGGCCTGCCCCATGGC44820.5018413164768993No Hit
GGTGAGCACTGAGGGAATGAAAGTGGGATCAGGGATCCACTTCCTTGCCC44670.5001617939987303No Hit
GTGAGGGGACTTGCTAAGTATTGTTAACTAATTTTAAATTAAACAAATTT43000.48146311040844864No Hit
TGGTGAAGTTTCATTTCATGAGGAAAAATTCACAAACAACAACCTTCAAA42300.47362533884366No Hit
TGAATGAAGAAGCAAATTCAAAACAGTAAACATTTCCTTTATACCTTTTT40700.4557104324098572No Hit
CTAAAGGCTGAATGAAAGGGTAATTCATATACTGAAGATCACACCCAAGC40260.4507838331405615No Hit
TTCTTTCAGCATACCACTTCATAACTGTTCAGTTTGTACAGTTTGTTCCC36920.41338646595999823No Hit
CAGCACTCTTTAGATAAACAGGTCATAAACAAGGAATTATATCAGACTAA36780.4118189116470405No Hit
GGAAGCTTCTAATAAAATACTCATCCATATATACCTTTTTCACAAGGTGA35110.3931202280567589No Hit
GTGTATTTATTGTAGCCGAGTATCTAATTAAACAAGTTTTTACTAAATCT34750.3890893741091533No Hit
AAGGTACAATGATTTCCACTTCTCTTATTTACATTTTCTAATCCCTTTCT34610.38752181979619554No Hit
TAAATAGTTGCCATTCCAAGTGTCTTATTTTTGTTCAAATTTATGTTTTT34480.3860662336484491No Hit
CAGTGGAGGTTAACATTCATCAAGATTAATAACTGGTGTACTTGATAGGC33290.3727420219883083No Hit
GTCAAAAAATCTGGAGTTCAGTTGGGATTTTATAATTGATTGTTAAACAT31930.35751435151957595No Hit
TATGTAAGACATTCTACTGCCATCTGCAGCATTCCAAATACTTCATGAAT31610.3539313702328154No Hit
GGAAGAACAGGATAGAAAGACTGCTTATATATTGGTCTAAATATGTTATT31440.3520279114242239No Hit
CTAAGTTTCAATATATAAATACGAATAAATCGAATAAATAGCCTTAAAAC31200.3493406754591534No Hit
AAACAAAAGTGTTGTCTTCATGCTAGTTTAAACTAATTTTTAAAAAATTA30220.3383677952684493No Hit
AACAGGTAGAAATAGCCCATGTCATTTAAAATTAGCACCCTGAGAAGCTC29950.33534465480774506No Hit
CCAATACGTGTTAAAAGCAAGTTACATTTTCTCTTTTAGGAAATTAACCA29440.32963427838197046No Hit
ACAGTCATGACCCACAGCAAACAGAACTGTTTTAGATATGCTGGGTATTC27980.3132869262611254No Hit
CTATTTCATATTTAACCACAGTTCTTTTCCCGTAGGCTGATCCTTATTCA26810.3001866509314072No Hit
GTGGTGGTATGTTCTAAGCTTTCTAATTTTTTTAATGTGACTATTTAGAA26770.2997387782705621No Hit
GTCCTTTGGTGAAGCTATTTATACATGTATATCTTAGGGTTCTGTTTTTA26210.29346856101873114No Hit
GTGTATTACCTTAATTTGAGTGATTCTTTAGATGTATTTAGTATTTGTAA26020.2913411658797171No Hit
CAAGTTACACTCTAGATCCTAAACGTAAGAAGCAACACTCATTACATTAC25880.28977361156675935No Hit
AGAAGGAGAGTGTCTGTCGCTCAGTCCCACTCAGGGGCCACTCTTCTTTG24980.2796964766977453No Hit
ACATAGAAAGCCAGGGTCCCAGATCAGCCACTCATTCCAGGGGAGGAAGG23450.2625653474204214No Hit
GATTCTGGCCTCTCTCCCTCTCAGGGCAGGGTGCCATTTGCCGTGCCTGA23190.2596541751249285No Hit
AAGACCAAGTCACTCTTTCTATGCAAAAACACTCACTCAGTTAACTGGTG23000.25752677998591444No Hit
CCTTATAGCATAGTGGGAGACAGACACATAAACAAGAAGGAAGAAGGTGT22260.24924113576028062No Hit
TACTACCCAGCTAAAATTATCATCTTTGTAATTAAAGCTATAGCTTAAAA22160.24812145410816797No Hit
CTGAGAGCATCTGGTTGGGAAGGACTGATCTAACAGCGGCTGGCAGCTGG21990.24621799529957644No Hit
GTTTTCTGTTGATATCTTTGATTACTTAACTTAAAAACAAAATAACTCCT21660.24252304584760462No Hit
GGAATGTTCTATTATTAAACTCATCATGTATACTTACAGGGCTTTTGGAA21300.23849219189999898No Hit
GTATTCTTTACATGGCTTTTGGTCTTCTAAGTGAAGCTTTTTGTTTTTCT21030.2354690514392948No Hit
ATATTTCAACAAAAAAATTATGCCTATTAGAATCAAAATATGATAGCAAA20670.23143819749168915No Hit
CATTCAAGATAGAGAAAACACTGTCTGCCGAGAATAATTGTTTTTATTTC19130.21419510004915404No Hit
ATCAAGCTGAGTGACCCTGGGGTCAGCCCCGCTGTGTTAAGCCTGGAGAG18740.20982834160591463No Hit
CCTATTTCTCCTTCCTAACAGTTTACCAAAGTTGAATCATATATAAGCTT18540.20758897830168926No Hit
CCCAATAGACCCACCCCAATCTCCCCAGACTGGATGTCAGTCTGCCCTTC18390.20590945582352024No Hit
TCCTATATGTGATCCGCAGTTGACTGAAATGTCACGCAGCGCATGACTGT18170.20344615618887238No Hit
GACTTCTGAATGAATTTATTTCAGAGTAATTTTCCAGAACTTACTGGTTG17750.19874349324999915No Hit
GTTTTATGTATGATCTCTTACCTATGACTCTACTGAAATAGAATTTCTAT16760.1876586448940837No Hit
TTTTCATTTTGGAAGTTCACTGGTCTATGAACAAAACTTTTTAAAACGAT16630.18620305874633725No Hit
CACTCAAATCCTTCTAACAATACTTTATATATTTTAAATACCATTTTGAA16440.18407566360732316No Hit
ATCATGTGTGATTTTGTAGTTCTGTTAAAGTTCATGGCTTTTGTGTTTTA15620.17489427405999927No Hit
TTCAAGCACAGGGTAGAATATTGGGCTGAGTAACACTTGCCTTCATCATG15600.1746703377295767No Hit
CATATAAGGCAAAGCATTAGGTACTTGGTTTATATATTAAAGATCTTACT15200.17019161112112605No Hit
GGGTGACAGAGAAAGATCCTATCTCAAAAAACAAAACAAGAAGAAGAAAA15130.16940783396464718No Hit
CCTCTTCTTTATTTTCAGAGTGTCTTTTCTTTTTTGCTACTAGAGTAAAA14580.1632495848780275No Hit
CATGGTAGTAGTATCAGTAGTAAAAGTATTTATTCCCATATGTCATTTTC14200.15899479459999935No Hit
CTCAAGTTTTCAGAAAAGAAGCCATGACATTAAATGTTAAAATACAGTAA14190.15888282643478807No Hit
GTATGTCCAAGATCAAAGTACACTGTAAAAAGCAATACTAAACTATAATT14110.15798708111309792No Hit
GGAGGCTGGCCTGAGACATTCCTATGTCCTGCTCGCCCACAGGCCTTCTT13620.15250064101774582No Hit
AAATGACTTAGTTCTGGTTGAGATGAAAGGATTCCACTGAAAGTTTTCTG13140.147126169087605No Hit
CTGTAAAATGGGGCCAGCAGCTATGTCTCGCTTGGGCTGGGATCCTGCAG13030.14589451927028108No Hit
GTCACTATTGGTAACAGAAAAGCTGCACTTTAGGATAACAAAGTCATACG12960.1451107421138022No Hit
TTCAAACCATCCTCCTGCCTCAGCCTCCCAAAGTGCTGGGATGACAGGCA12710.14231153798352053No Hit
CAGTAAAACACTAATCCAGCCAATAAAAAAAACAAACCGCTGCACAGCTG12420.1390644611923938No Hit
CCCTATATCTGTCATATTTTTATATAAAAATGTGTATATTAGTTTAATTG12390.13872855669675999No Hit
TCCACAGCACCCAAGCTCCCCAGCCAGGCCACCCACCCCTTTGAAAGAGA12000.13436179825352057No Hit
GCTCAAAAAAAAAAAAAAGAAAAAAGTGGATTTATTTTTATTTTACAGGT11940.13368998926225295No Hit
ACGCATCTTCTCTCCCTTCCTGTGTCTTCTACTTTTTGTGCTATTTTCAC11470.1284274854973234No Hit
ACCGGCATTGATTTGGGACCCACATGCCCGAGGAATACACTCACAGGCTC11470.1284274854973234No Hit
ATCTTTGACAATTACCTGATGAAATTAAAGTTTTTTTGTATTCTATAATA11400.12764370834084454No Hit
CATTTCTCTTGCTTACATGAACTCTATGTCGTGGCATTCAGATCAGTCAC11350.12708386751478817No Hit
AGAGATGGAATCAGTGATTTCAGATTGTTTGTTTCTTTTTTCTCCAGTTG10960.12271710907154879No Hit
TCCACAGCACCCAAGCTCGCCCATTGTTTCTGGGAATATCTGGCCAGGGG10960.12271710907154879No Hit
TAGAGATCTCTATCTCTTAATGACATTTCTTTTTCCCTCAGGCTTTCTGT10870.12170939558464736No Hit
GGAGGCTGGCCTGAGACTCACCCTTCAGGGAAGTGGGCTCCCCTCTGGCC10580.11846231879352064No Hit
TCTTTAATGGCCTTTTAAAATTAAAAGGTATTTAATCTGTAACTCCAGGT10540.11801444613267556No Hit
GGAGGCTGGCCTGAGACGGATGTCAGTTCTGCTCCCTTCCTGCCTCGGTC10480.11734263714140795No Hit
TCCACAGCACCCAAGCTCCCAGACAACATTTCTAGACATACCTTGGGCCA10040.11241603787211221No Hit
AGTGCAAATAGTGTATCTGACCTATTATCAATCATGTTTATACTTTTATT9790.10961683374183054No Hit
GAAGTATCATTCTCCATGAATGTCATATTGAGATTACATATAAGGTATCA9740.10905699291577418No Hit
GCAGTTTTAAAATCCTTTTTCTGTATGGGATTATGGAATATTTAAGTTAA9560.10704156594197138No Hit
CAGTTGTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAATCAAGTCA9440.10569794795943618No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)