FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001795575

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001795575
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences946848
Sequences flagged as poor quality0
Sequence length32-301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAGACCAGGTTCCAGACATGGCTATTTTTATGCCTACAGATATCATGG201712.1303313731454256No Hit
ACCGGCATTGATTTTGGGACCCACATGCCCGAGGAATACACTCACAGGCT185161.9555409104734869No Hit
TCCCTCATCCTCTCCCCATAGAAAAGTCCAACCTGATCGTGGTCCAGAGA184431.9478311196728513No Hit
GAAGAACTTGAGGCTCGTGGGCGCTCAGCCTTCTCTTGTGTCTTTTTAGA175301.8514059278786035No Hit
ATTTCCCAGGGCCTCTTACCCACTCCAGGCTGTCAGCAGGGATCTTGTGA159511.6846420967251343No Hit
CTCAAGTTTTCAGAAAAGAAGCCATGACATTAAATGTTAAAATACAGTAA143141.5117526783602013No Hit
ACTCTGGTTTCTGGTGGGACCATTATGGACATCAGGACATTCTCACCAAG125901.329674879178073No Hit
CATCAGATAAAATCCAAGAGCTTCTTCATTTAACGTAACTCACCATATGC102321.0806380749602893No Hit
AAACTTGCATTCGTATCCACAGATAGCAAAAGAAAAATAGGACCACAAAA99861.0546571360978743No Hit
CACATGGCATCTGTACAGTGTCTATAACAAAATAACCCAAAGTATGAGAT98591.041244212376221No Hit
CAGGAGCTTCCAAATAGTATGTTCTCATTAAAAGAGGTGTTCTTGTGACA92930.9814669302781439No Hit
GTCCTTTGGTGAAGCTATTTATACATGTATATCTTAGGGTTCTGTTTTTA79500.8396279022609754No Hit
GGAATGTTCTATTATTAAACTCATCATGTATACTTACAGGGCTTTTGGAA74030.7818572780425158No Hit
AATAACTGATGTGTTCTGTTAAGCTTATAAAGTTGAACTTTTTTTTTTTT72950.7704510122004798No Hit
CAAGTTACACTCTAGATCCTAAACGTAAGAAGCAACACTCATTACATTAC71680.7570380884788266No Hit
ATATATGGCTGTTGTGCCCTTCTCTTAGTGTTAATGAGTGCTTTTTATTT70080.7401399168609957No Hit
AGAAGGAGAGTGTCTGTCGCTCAGTCCCACTCAGGGGCCACTCTTCTTTG69720.7363378282469837No Hit
CAGTGGAGGTTAACATTCATCAAGATTAATAACTGGTGTACTTGATAGGC69550.7345423975125892No Hit
TTCAAGCACAGGGTAGAATATTGGGCTGAGTAACACTTGCCTTCATCATG69160.7304234681807429No Hit
CTCCCAAATTGCTGAGATTACAGATGTGAGCCACTGTGCCCAGCCTGATT67910.7172217716043124No Hit
CAGGGAGGAAAGGAGGACAGCCAACAAGTACCAGGCAGCATGGTCAACTG67840.7164824765960323No Hit
TCCTATATGTGATCCGCAGTTGACTGAAATGTCACGCAGCGCATGACTGT65440.6911352191692859No Hit
GTTTTATGTATGATCTCTTACCTATGACTCTACTGAAATAGAATTTCTAT64920.6856433133934908No Hit
CAGCACTCTTTAGATAAACAGGTCATAAACAAGGAATTATATCAGACTAA64420.6803626347629187No Hit
AAACAAAAGTGTTGTCTTCATGCTAGTTTAAACTAATTTTTAAAAAATTA64410.6802570211903072No Hit
GGAGGCTGGCCTGAGACATTCCTATGTCCTGCTCGCCCACAGGCCTTCTT63940.6752931832775694No Hit
ACAGTCATGACCCACAGCAAACAGAACTGTTTTAGATATGCTGGGTATTC63000.6653655074520937No Hit
GTACACCACACACACACACGCACACGCACACGCAGAGCTGAGGGAAGGCT62630.6614578052654703No Hit
GAGATACACAGTCTACCTGGTAAGAAAAAAAATAAAGTCTTCCTTATAAA61390.6483617222616512No Hit
AAAGGTTTCAGTGAGGTGAACAGTTTAAAGGCCTTGGGAATAAGAAAATC60830.6424473621954104No Hit
AGGGGAGGAGTTCAGAGGAAGACACTTGCCAGGGAAATATCCCTGTGGCT60510.6390677278718443No Hit
ATGAGGCCTCTTATACTGCCAAATCAATATAAAGAAGTAAAAGAAATCCC59490.6282951434654771No Hit
CCTTATAGCATAGTGGGAGACAGACACATAAACAAGAAGGAAGAAGGTGT59170.6249155091419109No Hit
AGATACGTTGACAACATTGGTGTGTAACAAAATCCGTATTTATAATGTGT56490.596611071682044No Hit
TCCACAGCACCCAAGCTCCCCAGCCAGGCCACCCACCCCTTTGAAAGAGA56190.5934426645037006No Hit
CCTGGTCTTTGAGGGGAACAACTTATATCTTACGTTTCATCTTATGTTTC55540.5865777822839569No Hit
ACATAGAAAGCCAGGGTCCCAGATCAGCCACTCATTCCAGGGGAGGAAGG55060.5815083307986076No Hit
TGCCAAACAACAAAGTGCTCAATCTACTATATGTACAAGAAGATTACCAA52100.5502467133056204No Hit
CATTTCTCTTGCTTACATGAACTCTATGTCGTGGCATTCAGATCAGTCAC50110.5292296123559431No Hit
CTAAAGGCTGAATGAAAGGGTAATTCATATACTGAAGATCACACCCAAGC50100.5291239987833316No Hit
AAACAGCAGCATGCTAATGAACTTAAATTACCTGTGAAGAATTGGAGGCA50090.5290183852107202No Hit
ATCATGTGTGATTTTGTAGTTCTGTTAAAGTTCATGGCTTTTGTGTTTTA49800.5259555916049884No Hit
AAGGTACAATGATTTCCACTTCTCTTATTTACATTTTCTAATCCCTTTCT49180.5194075501030788No Hit
GTTTTCTGTTGATATCTTTGATTACTTAACTTAAAAACAAAATAACTCCT48500.5122258271655007No Hit
GCCTCCCAAAGCATTATGAATATGGGCATGAGCCACTTCACCCAACCAAA48110.5081068978336544No Hit
TTTTCATTTTGGAAGTTCACTGGTCTATGAACAAAACTTTTTAAAACGAT48050.5074732163979858No Hit
GAAGTATCATTCTCCATGAATGTCATATTGAGATTACATATAAGGTATCA47930.5062058535266484No Hit
GGTGAGCAGTATTTTAAGAAGGTCCTGTTGTCAGTTTTTCAGATTTTCTT46740.49363783838588665No Hit
CCTCCAATAGCTTGCTTTTCACAATTGTCCTTTGTTTTGTTATAGTCCTG46570.4918424076514921No Hit
GGAAGAACAGGATAGAAAGACTGCTTATATATTGGTCTAAATATGTTATT46120.48708979688397713No Hit
TGGTGAAGTTTCATTTCATGAGGAAAAATTCACAAACAACAACCTTCAAA46100.4868785697387542No Hit
CATGGTAGTAGTATCAGTAGTAAAAGTATTTATTCCCATATGTCATTTTC45870.48444945756869107No Hit
AGTTGGGTACAGTCATGGTAATGCATTATATTTTAAGATTTTGCCTTTCT43830.46290428875595657No Hit
GATACTGGTTCTACTGTTTCTAAGTATGTGATTAAAATGTACATTGTTCT43340.45772922369799585No Hit
TTGCTATTCTCAGATGACTCTGTGTTTTTATAATAAAATAAACTGTACTT41520.4385075534827132No Hit
CAAACCACGCTCCTTCCACTGGGCCCAATATGACATCACAGCCCTCCCCA41210.4352335327317584No Hit
TTGAAATAGACATTGAAGGTGTCAACCAATAAACTTCTTTAAAAACTTGT40940.4323819662712495No Hit
TAGAGATCTCTATCTCTTAATGACATTTCTTTTTCCCTCAGGCTTTCTGT40870.4316426712629693No Hit
CCCCCACTTTGACAGAAGGGCAGACTGACATCCAGTCTGGGGAGATTGGG40550.42826303693940315No Hit
TAAATAGTTGCCATTCCAAGTGTCTTATTTTTGTTCAAATTTATGTTTTT38750.4092525938693433No Hit
GTCAGTCAGCGTGTTTTGGTCGATCTGGGTCTCAGCACATTACTGATGGG38320.40471121024705126No Hit
TTTGAGCTGTCTTGACGTTCACAGATATAAAATATTAAATATATTTTAAT38280.4042887559566055No Hit
CTAAAATGCCCTCCCCTCGAACCCTTACCAAACTCCTATGCATACTACAG35830.37841343066680183No Hit
CTGAGAGCATCTGGTTGGGAAGGACTGATCTAACAGCGGCTGGCAGCTGG35460.37450572848017843No Hit
AAAAACTCACGCGACAGTAATCTGTTAAGCCATTTATTTATAATTTATAA34780.3673240055426003No Hit
CATCACTAAAACTCTAAGGGCTAAGCCAGAGAAGGGAAGGCTCACCTTAA33880.3578187840075704No Hit
CATCTTTATTGCCCCTATATCTGTCATATTTTTATATAAAAATGTGTATA33540.3542279225387813No Hit
GGTGAGCACTGAGGGAATGAAAGTGGGATCAGGGATCCACTTCCTTGCCC32630.34461708743113995No Hit
GTGTATTTATTGTAGCCGAGTATCTAATTAAACAAGTTTTTACTAAATCT32440.34261042955152254No Hit
CAGTAAAACACTAATCCAGCCAATAAAAAAAACAAACCGCTGCACAGCTG32390.3420823616884653No Hit
CAGATTTTTGAAAAGTACTACTATGTTCTCTAATAAAAAATAGTAGATGA31780.33563993375916723No Hit
TAAGTGCAAATAGTGTATCTGACCTATTATCAATCATGTTTATACTTTTA30850.32581787150630304No Hit
TCACTTCAGGGCACAGAGAGGTCACCCAGCCTTCTGCTTCTTCCCTCAGG30810.32539541721585724No Hit
CATATGATAACAGCAAATACATGTTACTTAAAATTACCATTTCTCTCATT30550.3226494643279597No Hit
CTAAGTAACTATCTTAAGGGTTGCTCCAAAAATCTTACCTTTTCTAGATA29980.31662949068910745No Hit
CATTTTCCTGTGTCTGGCCCCCTTAGGAGGACAAAGGCCTGCCCCATGGC29680.3134610835107641No Hit
AAATGACTTAGTTCTGGTTGAGATGAAAGGATTCCACTGAAAGTTTTCTG29650.31314424279292985No Hit
GGATTCCATCTTAAATCCATCTTTCTCTAGAACTGAGTTTACAGTATTAA29230.3087084727432492No Hit
TCTTCATGCAGCTTCCCCTAATGTTAATATCTTACATACCCATGCCTGAC29110.30744110987191187No Hit
GGATTACGTTTGTGATTTTAAGCAGATTTACTTATTAGGCTCTATAACTA28060.2963516847477103No Hit
AGCAGTATGTTGAGTTTATGGCAGATTAATCTATCATCTTTTAGAAATTT27860.2942394132954814No Hit
GGGTGACAGAGAAAGATCCTATCTCAAAAAACAAAACAAGAAGAAGAAAA27760.293183277569367No Hit
GCAGTTTTAAAATCCTTTTTCTGTATGGGATTATGGAATATTTAAGTTAA27150.2867408496400689No Hit
CAGTTGTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAATCAAGTCA27110.28631839534962317No Hit
GTATTCTTTACATGGCTTTTGGTCTTCTAAGTGAAGCTTTTTGTTTTTCT26670.28167139815471964No Hit
TACTACCCAGCTAAAATTATCATCTTTGTAATTAAAGCTATAGCTTAAAA26600.2809321031464395No Hit
GATTCTGGCCTCTCTCCCTCTCAGGGCAGGGTGCCATTTGCCGTGCCTGA26460.2794535131298793No Hit
TGTTGGGTAGTTCCTTATGTAATGTTTTTTGTTTTTTATTAATAGGATCG25660.27100442732096386No Hit
TGAATGAAGAAGCAAATTCAAAACAGTAAACATTTCCTTTATACCTTTTT24930.2632946365203285No Hit
TCTTGTAGGAGTTAGGCCTTGAAAGAGAGATTTAATTGTTTTATTTGTTT24370.2573802764540877No Hit
TTCTTTCAGCATACCACTTCATAACTGTTCAGTTTGTACAGTTTGTTCCC24120.2547399371388016No Hit
GTATGTCCAAGATCAAAGTACACTGTAAAAAGCAATACTAAACTATAATT24010.2535781878400757No Hit
AGTGCAGTTCAGTCTCCCAACCCCGCCTCTCCCTGCTGCCAACCAGGCAC23520.248403122782115No Hit
GGCTACACTGGACACCTCATGGCTGTATGGCCTGGTCCAAGCCAGTGTCC23430.247452600628612No Hit
GTGAGGGGACTTGCTAAGTATTGTTAACTAATTTTAAATTAAACAAATTT23330.24639646490249756No Hit
TTGTTTGTTTGTTTGCTTGCTTGTTTTAAGATTGTTCCAGGACACGAAGG23070.24365051201460003No Hit
CTCCCTACAGAGTCAGCCACTTAAGCTGATAGAGACATGATGTAACCATG22670.23942596911014227No Hit
GCTCAAAAAAAAAAAAAAGAAAAAAGTGGATTTATTTTTATTTTACAGGT22320.23572949406874175No Hit
TTACAGTAAGTTTTGTTGGCTTACTTTAAAATTATTTCTCTCCTTATAAT22080.23319476832606711No Hit
CTAGTTCTGTTATGATGGATCAATGTTTTATTTTAAGGTAGAATTTTTAG21960.2319274054547298No Hit
CTCCCTACAGAGTCAGCCACTTAAGCTGATAGAGACATGATGTAACCGTG21470.22675234039676906No Hit
GTCACTATTGGTAACAGAAAAGCTGCACTTTAGGATAACAAAGTCATACG20760.2192537767413566No Hit
AGCTCTTTCTCCCTTTGCCTACTTCTCCCTCTAGTACAGAATACGCCATC20480.2162965967082362No Hit
TAAGCAGTCACTACCATTGTATTCTATATCAACATGCTTTTATTTTGATA18130.19147740714454695No Hit
GGAAGCTTCTAATAAAATACTCATCCATATATACCTTTTTCACAAGGTGA18070.1908437257088783No Hit
GGAGTTTCACCATATTGGCCAGGCTGTTCTCAAACTCCTGACCTTGTGAT17870.18873145425664942No Hit
ATATTTCAACAAAAAAATTATGCCTATTAGAATCAAAATATGATAGCAAA17690.18683040994964345No Hit
CTAAGTTTCAATATATAAATACGAATAAATCGAATAAATAGCCTTAAAAC17600.18587988779614045No Hit
GTGGTGGTATGTTCTAAGCTTTCTAATTTTTTTAATGTGACTATTTAGAA16770.17711396126939066No Hit
CCTCTTCTTTATTTTCAGAGTGTCTTTTCTTTTTTGCTACTAGAGTAAAA16400.17320625908276724No Hit
GTCAAAAAATCTGGAGTTCAGTTGGGATTTTATAATTGATTGTTAAACAT16260.17172766906620704No Hit
GAATGTTGTTTCTAGGTCCTACTCTAAATAATATTAACAAGCATTTAAAT15390.16253928824901145No Hit
ATCTTGGGTGCTGTGCTCAGACTCCCCCAATCTCTTCCACAGAAATCTCC14920.15757545033627363No Hit
AACAGGTAGAAATAGCCCATGTCATTTAAAATTAGCACCCTGAGAAGCTC14690.15514633816621043No Hit
CCAATACGTGTTAAAAGCAAGTTACATTTTCTCTTTTAGGAAATTAACCA14340.1514498631248099No Hit
CAGTTGTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAATCAAGTCAA14080.14870391023691237No Hit
GACTTCTGAATGAATTTATTTCAGAGTAATTTTCCAGAACTTACTGGTTG13960.14743654736557504No Hit
TACCCTTATTGAGACAATGCCAACATTAATTTTAAAAACCAAAAATTCTA13790.1456411166311805No Hit
AAGACCAAGTCACTCTTTCTATGCAAAAACACTCACTCAGTTAACTGGTG13650.14416252661462028No Hit
TTTCCCCAAACACAGCCTTCCTTCCCCCAGATCCCCAAGCTGCTCCATCG13530.14289516374328298No Hit
GTGTATTACCTTAATTTGAGTGATTCTTTAGATGTATTTAGTATTTGTAA13510.14268393659806008No Hit
CAGGAAATTTCTAAATGTGACATGACCTACTTACTGTACCTGGTGACAGA11210.11839281489742809No Hit
TTTATAAGCTTAACAGAACACATCAGTTATTTTATAACTTTAAAGTGAAC11200.11828720132481667No Hit
CCCAATAGACCCACCCCAATCTCCCCAGACTGGATGTCAGTCTGCCCTTC10880.11490756700125047No Hit
GCCAATATTTAACCAATTTTGACCTACATAAAAATACTATTTCAAATGGT10850.11459072628341614No Hit
CATTCAAGATAGAGAAAACACTGTCTGCCAAGAATAATTGTTTTTATTTC10760.11364020412991313No Hit
CACTCAAATCCTTCTAACAATACTTTATATATTTTAAATACCATTTTGAA10720.11321774983946736No Hit
CTATTTCATATTTAACCACAGTTCTTTTCCCGTAGGCTGATCCTTATTCA9640.10181148399743148No Hit
TGGGAGCTAGCAGTGTAAACAGAGTACATACATAAAAATTACATTTTAAT9610.10149464327959715No Hit
ATCAAGCTGAGTGACCCTGGGGTCAGCCCCGCTGTGTTAAGCCTGGAGAG9520.10054412112609416No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)