FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001795579

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001795579
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences868903
Sequences flagged as poor quality0
Sequence length32-301
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAAATGCCCTCCCCTCGAACCCTTACCAAACTCCTATGCATACTACAG119211.3719598159978732No Hit
GGTGAGCACTGAGGGAATGAAAGTGGGATCAGGGATCCACTTCCTTGCCC116351.3390447495290039No Hit
CATTTTCCTGTGTCTGGCCCCCTTAGGAGGACAAAGGCCTGCCCCATGGC113981.3117689776649408No Hit
CATGGTAGTAGTATCAGTAGTAAAAGTATTTATTCCCATATGTCATTTTC103471.1908118627740958No Hit
CAGCACTCTTTAGATAAACAGGTCATAAACAAGGAATTATATCAGACTAA103261.1883950222291786No Hit
CTCAAGTTTTCAGAAAAGAAGCCATGACATTAAATGTTAAAATACAGTAA100591.1576666210152342No Hit
AGTGCAGTTCAGTCTCCCAACCCCGCCTCTCCCTGCTGCCAACCAGGCAC99841.1490350476405307No Hit
CAGGAAATTTCTAAATGTGACATGACCTACTTACTGTACCTGGTGACAGA94821.091261049852515No Hit
AAGGTACAATGATTTCCACTTCTCTTATTTACATTTTCTAATCCCTTTCT91481.0528217764238357No Hit
AAACAGCAGCATGCTAATGAACTTAAATTACCTGTGAAGAATTGGAGGCA90091.0368245937693852No Hit
CCTCCAATAGCTTGCTTTTCACAATTGTCCTTTGTTTTGTTATAGTCCTG88721.0210575864049267No Hit
AAACAAAAGTGTTGTCTTCATGCTAGTTTAAACTAATTTTTAAAAAATTA88161.014612678285148No Hit
TGGGAGCTAGCAGTGTAAACAGAGTACATACATAAAAATTACATTTTAAT84230.9693832338017017No Hit
GTCCTTTGGTGAAGCTATTTATACATGTATATCTTAGGGTTCTGTTTTTA83150.9569537681421287No Hit
CATTTCTCTTGCTTACATGAACTCTATGTCGTGGCATTCAGATCAGTCAC82190.9459053542225081No Hit
GTTTTATGTATGATCTCTTACCTATGACTCTACTGAAATAGAATTTCTAT80570.9272611557331487No Hit
CAGTGGAGGTTAACATTCATCAAGATTAATAACTGGTGTACTTGATAGGC79770.9180541441334648No Hit
TCCTATATGTGATCCGCAGTTGACTGAAATGTCACGCAGCGCATGACTGT78880.9078113437288167No Hit
ACAGTCATGACCCACAGCAAACAGAACTGTTTTAGATATGCTGGGTATTC77990.8975685433241685No Hit
GGATTACGTTTGTGATTTTAAGCAGATTTACTTATTAGGCTCTATAACTA76760.8834127629896547No Hit
CCTCTTCTTTATTTTCAGAGTGTCTTTTCTTTTTTGCTACTAGAGTAAAA76730.8830675000546666No Hit
TTCAAGCACAGGGTAGAATATTGGGCTGAGTAACACTTGCCTTCATCATG74480.8571727799305561No Hit
GTCACTATTGGTAACAGAAAAGCTGCACTTTAGGATAACAAAGTCATACG74040.85210892355073No Hit
TGTTGGGTAGTTCCTTATGTAATGTTTTTTGTTTTTTATTAATAGGATCG73450.8453187524959632No Hit
CAGTAAAACACTAATCCAGCCAATAAAAAAAACAAACCGCTGCACAGCTG72880.8387587567311887No Hit
TTCTTTCAGCATACCACTTCATAACTGTTCAGTTTGTACAGTTTGTTCCC70780.8145903512820188No Hit
AGATACGTTGACAACATTGGTGTGTAACAAAATCCGTATTTATAATGTGT70510.8114829848671256No Hit
GGATTCCATCTTAAATCCATCTTTCTCTAGAACTGAGTTTACAGTATTAA70010.8057286026173233No Hit
AGCAGTATGTTGAGTTTATGGCAGATTAATCTATCATCTTTTAGAAATTT68870.7926086110877738No Hit
CAAGTTACACTCTAGATCCTAAACGTAAGAAGCAACACTCATTACATTAC67940.7819054601031417No Hit
CCTTATAGCATAGTGGGAGACAGACACATAAACAAGAAGGAAGAAGGTGT67850.7808696712981771No Hit
GGTGAGCAGTATTTTAAGAAGGTCCTGTTGTCAGTTTTTCAGATTTTCTT67110.7723531855684697No Hit
GTTTTCTGTTGATATCTTTGATTACTTAACTTAAAAACAAAATAACTCCT65980.7593482816839163No Hit
GAGATACACAGTCTACCTGGTAAGAAAAAAAATAAAGTCTTCCTTATAAA65340.7519826724041694No Hit
TAGAGATCTCTATCTCTTAATGACATTTCTTTTTCCCTCAGGCTTTCTGT64450.7417398719995212No Hit
CTAAAGGCTGAATGAAAGGGTAATTCATATACTGAAGATCACACCCAAGC63440.7301160198549205No Hit
TGAATGAAGAAGCAAATTCAAAACAGTAAACATTTCCTTTATACCTTTTT62380.7179167294853396No Hit
AAGAGATGGAATCAGTGATTTCAGATTGTTTGTTTCTTTTTTCTCCAGTT61860.7119321719455451No Hit
AGGCAAACAACATTCCATGATGACCAAATATTTACTAAAAAGAATAGATA57100.6571504529274269No Hit
TGCCAAACAACAAAGTGCTCAATCTACTATATGTACAAGAAGATTACCAA55490.6386213420830633No Hit
CAGGAGCTTCCAAATAGTATGTTCTCATTAAAAGAGGTGTTCTTGTGACA54780.630450119288344No Hit
GAAGTATCATTCTCCATGAATGTCATATTGAGATTACATATAAGGTATCA54650.6289539799033955No Hit
CATCAGATAAAATCCAAGAGCTTCTTCATTTAACGTAACTCACCATATGC53610.6169848648238065No Hit
CCAATACGTGTTAAAAGCAAGTTACATTTTCTCTTTTAGGAAATTAACCA53160.6118059207989844No Hit
AACAGGTAGAAATAGCCCATGTCATTTAAAATTAGCACCCTGAGAAGCTC51220.5894789176697515No Hit
ATCATGTGTGATTTTGTAGTTCTGTTAAAGTTCATGGCTTTTGTGTTTTA49910.5744024361752692No Hit
CCTATTTCTCCTTCCTAACAGTTTACCAAAGTTGAATCATATATAAGCTT48620.5595561299707792No Hit
GCCTCCCAAAGCATTATGAATATGGGCATGAGCCACTTCACCCAACCAAA48600.5593259546807872No Hit
AAACTTGCATTCGTATCCACAGATAGCAAAAGAAAAATAGGACCACAAAA47130.5424080708663683No Hit
GGAAGCTTCTAATAAAATACTCATCCATATATACCTTTTTCACAAGGTGA46430.534351935716645No Hit
ATATATGGCTGTTGTGCCCTTCTCTTAGTGTTAATGAGTGCTTTTTATTT43640.502242482762748No Hit
CATATAAGGCAAAGCATTAGGTACTTGGTTTATATATTAAAGATCTTACT42530.4894677541681868No Hit
CTCCCAAATTGCTGAGATTACAGATGTGAGCCACTGTGCCCAGCCTGATT40530.4664502251689774No Hit
CAGTTGTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAATCAAGTCA40120.4617316317241395No Hit
GTCAAAAAATCTGGAGTTCAGTTGGGATTTTATAATTGATTGTTAAACAT39780.45781865179427395No Hit
GAATTTGTTCTTACAAAAGATAGAGTATACTAAATTATTTATGAAATATA39010.4489569031295783No Hit
AATAACTGATGTGTTCTGTTAAGCTTATAAAGTTGAACTTTTTTTTTTTT37490.43146358109017924No Hit
TTGCTATTCTCAGATGACTCTGTGTTTTTATAATAAAATAAACTGTACTT37390.4303127046402188No Hit
CATCTTTATTGCCCCTATATCTGTCATATTTTTATATAAAAATGTGTATA37370.4300825293502267No Hit
ATGAGGCCTCTTATACTGCCAAATCAATATAAAGAAGTAAAAGAAATCCC36570.42087551775054294No Hit
AAGACCAAGTCACTCTTTCTATGCAAAAACACTCACTCAGTTAACTGGTG34830.40085026752123076No Hit
CATATGATAACAGCAAATACATGTTACTTAAAATTACCATTTCTCTCATT33970.39095273005157083No Hit
TTTGAGCTGTCTTGACGTTCACAGATATAAAATATTAAATATATTTTAAT32690.3762215114920768No Hit
TGGTGAAGTTTCATTTCATGAGGAAAAATTCACAAACAACAACCTTCAAA31680.36459765934747607No Hit
GTGTATTACCTTAATTTGAGTGATTCTTTAGATGTATTTAGTATTTGTAA30630.35251345662289113No Hit
TTGAAATAGACATTGAAGGTGTCAACCAATAAACTTCTTTAAAAACTTGT30160.34710433730807694No Hit
CTATTTCATATTTAACCACAGTTCTTTTCCCGTAGGCTGATCCTTATTCA29640.34111977976828256No Hit
TATGTAAGACATTCTACTGCCATCTGCAGCATTCCAAATACTTCATGAAT29600.3406594291882984No Hit
GTGAGGGGACTTGCTAAGTATTGTTAACTAATTTTAAATTAAACAAATTT29410.33847276393337344No Hit
GTGTATTTATTGTAGCCGAGTATCTAATTAAACAAGTTTTTACTAAATCT29050.3343296087135158No Hit
CCCCCACTTTGACAGAAGGGCAGACTGACATCCAGTCTGGGGAGATTGGG28810.33156750523361067No Hit
CAAACCACGCTCCTTCCACTGGGCCCAATATGACATCACAGCCCTCCCCA28020.32247558127892295No Hit
GCCAATATTTAACCAATTTTGACCTACATAAAAATACTATTTCAAATGGT27800.3199436530890099No Hit
CATCAAATGCTCTTTAATGGCCTTTTAAAATTAAAAGGTATTTAATCTGT25900.29807700053976105No Hit
TAAATAGTTGCCATTCCAAGTGTCTTATTTTTGTTCAAATTTATGTTTTT25590.29450928354488365No Hit
AAAGGTTTCAGTGAGGTGAACAGTTTAAAGGCCTTGGGAATAAGAAAATC25550.2940489329648994No Hit
GAATGTTGTTTCTAGGTCCTACTCTAAATAATATTAACAAGCATTTAAAT25180.2897906901000457No Hit
GGAAGAACAGGATAGAAAGACTGCTTATATATTGGTCTAAATATGTTATT25080.28863981365008523No Hit
GTATCTTTGACAATTACCTGATGAAATTAAAGTTTTTTTGTATTCTATAA24460.2815043796603303No Hit
GAATACATTTCATTCAAATTTATCCGAAACTTTATTAGTATAAATTCTCC23860.27459912096056754No Hit
GGGTGACAGAGAAAGATCCTATCTCAAAAAACAAAACAAGAAGAAGAAAA22910.2636657946859431No Hit
AGTTGGGTACAGTCATGGTAATGCATTATATTTTAAGATTTTGCCTTTCT22410.25791141243614074No Hit
CTGGGGCTGTCATATAGCGCTGATCCCCCCAGTTGTGCATTTACAATGGA22180.2552643966012317No Hit
TTGTTTGTTTGTTTGCTTGCTTGTTTTAAGATTGTTCCAGGACACGAAGG21780.2506608908013898No Hit
CAGATTTTTGAAAAGTACTACTATGTTCTCTAATAAAAAATAGTAGATGA21220.24421598268161118No Hit
TCCTTTTAGTTTGTTAATGTGATGGAATAGTTTTCAAATTATCCTTTTTT21130.24318019387664677No Hit
AAATGACTTAGTTCTGGTTGAGATGAAAGGATTCCACTGAAAGTTTTCTG21110.2429500185866547No Hit
GGAATGTTCTATTATTAAACTCATCATGTATACTTACAGGGCTTTTGGAA20320.23385809463196697No Hit
ACTATGAAAATGATCAGGGATATGTGAGTGTGTGTGTGTCTGTGTGTGTT20100.23132616644205395No Hit
GATACTGGTTCTACTGTTTCTAAGTATGTGATTAAAATGTACATTGTTCT19660.22626231006222788No Hit
CAGTTGTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAATCAAGTCAA19650.22614722241723184No Hit
ACTATTCCTGCTTGACCTTCAATGCTGTTCCTCAGTTTGTCACTAAAATC19650.22614722241723184No Hit
GTATGTCCAAGATCAAAGTACACTGTAAAAAGCAATACTAAACTATAATT18870.2171703861075402No Hit
GTATTTCCATTTCTTAGAGGGAATGGTATATATACTTTATATAATATATA18660.2147535455626232No Hit
TTGTGCTAATAGAGGAGCACTGTCTTAAAATAACTTACTTGCTTAGATGT17990.20704267334788806No Hit
AAAAACTCACGCGACAGTAATCTGTTAAGCCATTTATTTATAATTTATAA16680.19196619185340597No Hit
TACTACCCAGCTAAAATTATCATCTTTGTAATTAAAGCTATAGCTTAAAA16530.19023987717846527No Hit
TTACAGTAAGTTTTGTTGGCTTACTTTAAAATTATTTCTCTCCTTATAAT16260.187132510763572No Hit
GTATTCTTTACATGGCTTTTGGTCTTCTAAGTGAAGCTTTTTGTTTTTCT16240.18690233547357993No Hit
GCTCAAAAAAAAAAAAAAGAAAAAAGTGGATTTATTTTTATTTTACAGGT15850.18241391731873408No Hit
CCGCAGTTGACTGAAATGTCACGCAGCGCATGACTGTAGTTTACCTAGAA15410.17735006093890804No Hit
CACATGGCATCTGTACAGTGTCTATAACAAAATAACCCAAAGTATGAGAT15080.17355216865403847No Hit
CATCACTAAAACTCTAAGGGCTAAGCCAGAGAAGGGAAGGCTCACCTTAA14860.17102024046412545No Hit
GAATACATTTCATTCAAATTTATCCGAAACTGTATTAGTATAAATTCTCC14670.16883357520920056No Hit
TCTTGTAGGAGTTAGGCCTTGAAAGAGAGATTTAATTGTTTTATTTGTTT14150.16284901766940613No Hit
TTTTCATTTTGGAAGTTCACTGGTCTATGAACAAAACTTTTTAAAACGAT13480.155138145454671No Hit
GGAAGCAAAGATAAATGTTAAGACTTACACACAAAAGTAATATCACAACA12260.14109745276515331No Hit
TAAGTGCAAATAGTGTATCTGACCTATTATCAATCATGTTTATACTTTTA12130.13960131338020468No Hit
ACGCATCTTCTCTCCCTTCCTGTGTCTTCTACTTTTTGTGCTATTTTCAC11730.13499780758036284No Hit
TAAGCAGTCACTACCATTGTATTCTATATCAACATGCTTTTATTTTGATA11170.1285528994605842No Hit
GACTTCTGAATGAATTTATTTCAGAGGTCGTATTGTTAGAAGGATTTGAG11030.12694167243063956No Hit
GCAGTTTTAAAATCCTTTTTCTGTATGGGATTATGGAATATTTAAGTTAA10640.12245325427579373No Hit
CACACCTCTTTCTCTCTATATATGCATATATACATATACATATATATACC10110.11635360909100324No Hit
CCAGCCAATAAAAAAAACAAACCGCTGCACAGCTGGCATCCAACTTCTTG10060.11577817086602302No Hit
CTAGTTCTGTTATGATGGATCAATGTTTTATTTTAAGGTAGAATTTTTAG10050.11566308322102697No Hit
CCATGAATGTCATATTGAGATTACATATAAGGTATCAATAAATACTCACC9620.11071431448619695No Hit
TACCCTTATTGAGACAATGCCAACATTAATTTTAAAAACCAAAAATTCTA9100.10472975694640255No Hit
CAGGATATGCCACCTTTAACTCAGTTAACTGAACTTTTGTTTTTTAATAT8760.10081677701653693No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)