FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001795581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001795581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences672721
Sequences flagged as poor quality0
Sequence length32-301
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCAAGCACAGGGTAGAATATTGGGCTGAGTAACACTTGCCTTCATCATG114621.703826697843534No Hit
GCCTCCCAAAGCATTATGAATATGGGCATGAGCCACTTCACCCAACCAAA103491.5383792092115454No Hit
CCTCCAATAGCTTGCTTTTCACAATTGTCCTTTGTTTTGTTATAGTCCTG102771.527676406712441No Hit
AAACAGCAGCATGCTAATGAACTTAAATTACCTGTGAAGAATTGGAGGCA102291.5205412050463714No Hit
CATGGTAGTAGTATCAGTAGTAAAAGTATTTATTCCCATATGTCATTTTC97101.443391837031994No Hit
CAGCACTCTTTAGATAAACAGGTCATAAACAAGGAATTATATCAGACTAA89231.3264042597153947No Hit
TTGCTATTCTCAGATGACTCTGTGTTTTTATAATAAAATAAACTGTACTT78751.170619023339542No Hit
AAGGTACAATGATTTCCACTTCTCTTATTTACATTTTCTAATCCCTTTCT78451.1661595222982486No Hit
GTCACTATTGGTAACAGAAAAGCTGCACTTTAGGATAACAAAGTCATACG75521.122605062128282No Hit
TTCTTTCAGCATACCACTTCATAACTGTTCAGTTTGTACAGTTTGTTCCC73261.089010154283871No Hit
CCTCTTCTTTATTTTCAGAGTGTCTTTTCTTTTTTGCTACTAGAGTAAAA73221.0884155541450318No Hit
ATCATGTGTGATTTTGTAGTTCTGTTAAAGTTCATGGCTTTTGTGTTTTA73081.0863344536590949No Hit
GTTTTATGTATGATCTCTTACCTATGACTCTACTGAAATAGAATTTCTAT70981.0551179463700406No Hit
GGTGAGCACTGAGGGAATGAAAGTGGGATCAGGGATCCACTTCCTTGCCC67240.9995228333885817No Hit
CAGTAAAACACTAATCCAGCCAATAAAAAAAACAAACCGCTGCACAGCTG66740.9920903316530925No Hit
TGAATGAAGAAGCAAATTCAAAACAGTAAACATTTCCTTTATACCTTTTT65730.9770766781474044No Hit
CATTTCTCTTGCTTACATGAACTCTATGTCGTGGCATTCAGATCAGTCAC65640.9757388278350163No Hit
CAAGTTACACTCTAGATCCTAAACGTAAGAAGCAACACTCATTACATTAC63870.9494277716913847No Hit
CCAATACGTGTTAAAAGCAAGTTACATTTTCTCTTTTAGGAAATTAACCA62820.9338195180468575No Hit
AAACTTGCATTCGTATCCACAGATAGCAAAAGAAAAATAGGACCACAAAA62770.9330762678733084No Hit
CATTTTCCTGTGTCTGGCCCCCTTAGGAGGACAAAGGCCTGCCCCATGGC59550.8852109566967584No Hit
GGATTACGTTTGTGATTTTAAGCAGATTTACTTATTAGGCTCTATAACTA59230.8804541555860455No Hit
CAGGAAATTTCTAAATGTGACATGACCTACTTACTGTACCTGGTGACAGA58500.8696027030522312No Hit
AACAGGTAGAAATAGCCCATGTCATTTAAAATTAGCACCCTGAGAAGCTC58000.862170201316742No Hit
CTCAAGTTTTCAGAAAAGAAGCCATGACATTAAATGTTAAAATACAGTAA55250.8212914417715517No Hit
TGTTGGGTAGTTCCTTATGTAATGTTTTTTGTTTTTTATTAATAGGATCG52650.7826424327470081No Hit
AATAACTGATGTGTTCTGTTAAGCTTATAAAGTTGAACTTTTTTTTTTTT51700.7685206794495787No Hit
TAGAGATCTCTATCTCTTAATGACATTTCTTTTTCCCTCAGGCTTTCTGT50920.7569259767422155No Hit
AAGAGATGGAATCAGTGATTTCAGATTGTTTGTTTCTTTTTTCTCCAGTT50780.7548448762562786No Hit
TGGGAGCTAGCAGTGTAAACAGAGTACATACATAAAAATTACATTTTAAT49900.7417636732018177No Hit
GAAGTATCATTCTCCATGAATGTCATATTGAGATTACATATAAGGTATCA48530.7213986184465774No Hit
ACAGTCATGACCCACAGCAAACAGAACTGTTTTAGATATGCTGGGTATTC48480.7206553682730285No Hit
AGGCAAACAACATTCCATGATGACCAAATATTTACTAAAAAGAATAGATA47390.7044525144896621No Hit
CAGTGGAGGTTAACATTCATCAAGATTAATAACTGGTGTACTTGATAGGC45280.6730873571658978No Hit
CCTTATAGCATAGTGGGAGACAGACACATAAACAAGAAGGAAGAAGGTGT45180.6716008568188001No Hit
CAGGAGCTTCCAAATAGTATGTTCTCATTAAAAGAGGTGTTCTTGTGACA43110.640830299633875No Hit
GGTGAGCAGTATTTTAAGAAGGTCCTGTTGTCAGTTTTTCAGATTTTCTT42270.6283436967182532No Hit
CTAAAGGCTGAATGAAAGGGTAATTCATATACTGAAGATCACACCCAAGC41830.6218030951910227No Hit
GTCCTTTGGTGAAGCTATTTATACATGTATATCTTAGGGTTCTGTTTTTA39260.5836000362706085No Hit
CCCAATAGACCCACCCCAATCTCCCCAGACTGGATGTCAGTCTGCCCTTC38040.565464732036015No Hit
GTTTTCTGTTGATATCTTTGATTACTTAACTTAAAAACAAAATAACTCCT37700.5604106308558823No Hit
TAAGCAGTCACTACCATTGTATTCTATATCAACATGCTTTTATTTTGATA37650.5596673806823333No Hit
GGAAGAACAGGATAGAAAGACTGCTTATATATTGGTCTAAATATGTTATT37210.5531267791551029No Hit
CCCCCACTTTGACAGAAGGGCAGACTGACATCCAGTCTGGGGAGATTGGG35420.5265184229420518No Hit
AAACAAAAGTGTTGTCTTCATGCTAGTTTAAACTAATTTTTAAAAAATTA35210.5233967722131463No Hit
CCTATTTCTCCTTCCTAACAGTTTACCAAAGTTGAATCATATATAAGCTT34460.5122480196099126No Hit
ATCAAGCTGAGTGACCCTGGGGTCAGCCCCGCTGTGTTAAGCCTGGAGAG34240.5089777188462974No Hit
CAAACCACGCTCCTTCCACTGGGCCCAATATGACATCACAGCCCTCCCCA33840.503031717457906No Hit
AAATGACTTAGTTCTGGTTGAGATGAAAGGATTCCACTGAAAGTTTTCTG33790.502288467284357No Hit
GGAAGCTTCTAATAAAATACTCATCCATATATACCTTTTTCACAAGGTGA33610.49961276665958104No Hit
AAAAACTCACGCGACAGTAATCTGTTAAGCCATTTATTTATAATTTATAA32670.48563966339686143No Hit
GGAATGTTCTATTATTAAACTCATCATGTATACTTACAGGGCTTTTGGAA32660.4854910133621516No Hit
TACCCTTATTGAGACAATGCCAACATTAATTTTAAAAACCAAAAATTCTA31870.4737476606200787No Hit
CATATAAGGCAAAGCATTAGGTACTTGGTTTATATATTAAAGATCTTACT30470.45293665576070913No Hit
TCCTATATGTGATCCGCAGTTGACTGAAATGTCACGCAGCGCATGACTGT30110.44758525451115694No Hit
GTGTATTTATTGTAGCCGAGTATCTAATTAAACAAGTTTTTACTAAATCT29430.43747705215089167No Hit
AAGACCAAGTCACTCTTTCTATGCAAAAACACTCACTCAGTTAACTGGTG29380.4367338019773428No Hit
GGGTGACAGAGAAAGATCCTATCTCAAAAAACAAAACAAGAAGAAGAAAA29060.4319770008666297No Hit
CTGGGGCTGTCATATAGCGCTGATCCCCCCAGTTGTGCATTTACAATGGA28690.4264769495823677No Hit
CATCAGATAAAATCCAAGAGCTTCTTCATTTAACGTAACTCACCATATGC27730.41220654625022857No Hit
GTATGTCCAAGATCAAAGTACACTGTAAAAAGCAATACTAAACTATAATT25880.38470628982891864No Hit
TCCTTTTAGTTTGTTAATGTGATGGAATAGTTTTCAAATTATCCTTTTTT25260.3754899876769121No Hit
TCTTGTAGGAGTTAGGCCTTGAAAGAGAGATTTAATTGTTTTATTTGTTT24610.36582773542077623No Hit
GTCAAAAAATCTGGAGTTCAGTTGGGATTTTATAATTGATTGTTAAACAT24400.36270608469187077No Hit
TGGTGAAGTTTCATTTCATGAGGAAAAATTCACAAACAACAACCTTCAAA24230.36017903410180446No Hit
GCCAATATTTAACCAATTTTGACCTACATAAAAATACTATTTCAAATGGT23910.35542223299109144No Hit
CATATGATAACAGCAAATACATGTTACTTAAAATTACCATTTCTCTCATT23860.35467898281754245No Hit
GTGTATTACCTTAATTTGAGTGATTCTTTAGATGTATTTAGTATTTGTAA23050.34263833000605004No Hit
TTTTCATTTTGGAAGTTCACTGGTCTATGAACAAAACTTTTTAAAACGAT23010.34204372986721093No Hit
CAGTTGTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAATCAAGTCA22920.34070587955482284No Hit
TGCCAAACAACAAAGTGCTCAATCTACTATATGTACAAGAAGATTACCAA22650.33669232861765874No Hit
GGATTCCATCTTAAATCCATCTTTCTCTAGAACTGAGTTTACAGTATTAA22460.33386797795817286No Hit
ACTATGAAAATGATCAGGGATATGTGAGTGTGTGTGTGTCTGTGTGTGTT22430.3334220278540435No Hit
CATCAAATGCTCTTTAATGGCCTTTTAAAATTAAAAGGTATTTAATCTGT22220.33030037712513804No Hit
ATATATGGCTGTTGTGCCCTTCTCTTAGTGTTAATGAGTGCTTTTTATTT21270.31617862382770867No Hit
GTGAGGGGACTTGCTAAGTATTGTTAACTAATTTTAAATTAAACAAATTT20940.3112731726822858No Hit
AGCAGTATGTTGAGTTTATGGCAGATTAATCTATCATCTTTTAGAAATTT20660.3071109717104119No Hit
TAAATAGTTGCCATTCCAAGTGTCTTATTTTTGTTCAAATTTATGTTTTT20650.30696232167570214No Hit
GAATGTTGTTTCTAGGTCCTACTCTAAATAATATTAACAAGCATTTAAAT19830.2947730188294999No Hit
GTATCTTTGACAATTACCTGATGAAATTAAAGTTTTTTTGTATTCTATAA19730.29328651848240206No Hit
TATGTAAGACATTCTACTGCCATCTGCAGCATTCCAAATACTTCATGAAT19660.2922459682394336No Hit
AGATACGTTGACAACATTGGTGTGTAACAAAATCCGTATTTATAATGTGT19530.2903135177882064No Hit
CTATTTCATATTTAACCACAGTTCTTTTCCCGTAGGCTGATCCTTATTCA19080.28362426622626613No Hit
GCTCAAAAAAAAAAAAAAGAAAAAAGTGGATTTATTTTTATTTTACAGGT18750.27871881508084334No Hit
GAGATACACAGTCTACCTGGTAAGAAAAAAAATAAAGTCTTCCTTATAAA18640.2770836646990357No Hit
CATCACTAAAACTCTAAGGGCTAAGCCAGAGAAGGGAAGGCTCACCTTAA18480.2747052641436792No Hit
TTGTTTGTTTGTTTGCTTGCTTGTTTTAAGATTGTTCCAGGACACGAAGG17990.2674214124428998No Hit
GGAAGCAAAGATAAATGTTAAGACTTACACACAAAAGTAATATCACAACA17770.26415111167928457No Hit
TACTACCCAGCTAAAATTATCATCTTTGTAATTAAAGCTATAGCTTAAAA17710.2632592114710259No Hit
ACTATTCCTGCTTGACCTTCAATGCTGTTCCTCAGTTTGTCACTAAAATC17350.2579078102214737No Hit
CACACCTCTTTCTCTCTATATATGCATATATACATATACATATATATACC17090.25404290931901935No Hit
GTATTCTTTACATGGCTTTTGGTCTTCTAAGTGAAGCTTTTTGTTTTTCT16970.2522591089025019No Hit
TAAGTGCAAATAGTGTATCTGACCTATTATCAATCATGTTTATACTTTTA16500.2452725572711421No Hit
GCAGTTTTAAAATCCTTTTTCTGTATGGGATTATGGAATATTTAAGTTAA15040.2235696522035138No Hit
CATCTTTATTGCCCCTATATCTGTCATATTTTTATATAAAAATGTGTATA14240.21167764942673115No Hit
ACGCATCTTCTCTCCCTTCCTGTGTCTTCTACTTTTTGTGCTATTTTCAC14030.20855599869782568No Hit
CAGATTTTTGAAAAGTACTACTATGTTCTCTAATAAAAAATAGTAGATGA13580.20186674713588548No Hit
CACATGGCATCTGTACAGTGTCTATAACAAAATAACCCAAAGTATGAGAT13040.19383964526155717No Hit
CTAGTTCTGTTATGATGGATCAATGTTTTATTTTAAGGTAGAATTTTTAG12730.18923149418555388No Hit
TTCAAACCATCCTCCTGCCTCAGCCTCCCAAAGTGCTGGGATGACAGGCA11730.17436649071457558No Hit
CAGTTGTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAATCAAGTCAA10990.16336638814605164No Hit
CTGTAAAATGGGGCCAGCAGCTATGTCTCGCTTGGGCTGGGATCCTGCAG10940.16262313797250272No Hit
TTTGAGCTGTCTTGACGTTCACAGATATAAAATATTAAATATATTTTAAT10510.15623118647998202No Hit
TTGAAATAGACATTGAAGGTGTCAACCAATAAACTTCTTTAAAAACTTGT10450.15533928627172333No Hit
AGTTGGGTACAGTCATGGTAATGCATTATATTTTAAGATTTTGCCTTTCT10370.1541500859940451No Hit
TTACAGTAAGTTTTGTTGGCTTACTTTAAAATTATTTCTCTCCTTATAAT10310.15325818578578637No Hit
CTAAGTTTCAATATATAAATACGAATAAATCGAATAAATAGCCTTAAAAC10270.15266358564694726No Hit
GTGGTGGTATGTTCTAAGCTTTCTAATTTTTTTAATGTGACTATTTAGAA9990.14850138467507332No Hit
CCAGCCAATAAAAAAAACAAACCGCTGCACAGCTGGCATCCAACTTCTTG9900.14716353436268526No Hit
GTATTTCCATTTCTTAGAGGGAATGGTATATATACTTTATATAATATATA9760.14508243387674832No Hit
TTGTGCTAATAGAGGAGCACTGTCTTAAAATAACTTACTTGCTTAGATGT9430.14017698273132548No Hit
GTTTTTATTTCTTTGTTGCTTGGTTCTTTGTTTGTCTTAATTGCAGAAGA9320.13854183234951786No Hit
TGTAGTTCTGTTAAAGTTCATGGCTTTTGTGTTTTACCTTAATTATTCTA9130.13571748169003198No Hit
CTCCCAAATTGCTGAGATTACAGATGTGAGCCACTGTGCCCAGCCTGATT9110.13542018162061242No Hit
CCATGAATGTCATATTGAGATTACATATAAGGTATCAATAAATACTCACC8520.1266498295727352No Hit
ATGAGGCCTCTTATACTGCCAAATCAATATAAAGAAGTAAAAGAAATCCC8290.1232308787744102No Hit
CATGTGTGATTTTGTAGTTCTGTTAAAGTTCATGGCTTTTGTGTTTTACC8150.12114977828847323No Hit
GAATCAAAATATGATAGCAAAACAGGAAGCATACTTACTAAACTTTTACA8140.12100112825376345No Hit
AAAGGTTTCAGTGAGGTGAACAGTTTAAAGGCCTTGGGAATAAGAAAATC6810.10123067363736231No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)