FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001795588

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001795588
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences843577
Sequences flagged as poor quality0
Sequence length32-301
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGAGCACTGAGGGAATGAAAGTGGGATCAGGGATCCACTTCCTTGCCC212922.5240138126098746No Hit
CATTTTCCTGTGTCTGGCCCCCTTAGGAGGACAAAGGCCTGCCCCATGGC207272.4570371169436815No Hit
AGTGCAGTTCAGTCTCCCAACCCCGCCTCTCCCTGCTGCCAACCAGGCAC146901.7413940873210152No Hit
CTAAAATGCCCTCCCCTCGAACCCTTACCAAACTCCTATGCATACTACAG141221.6740617631822583No Hit
CCAATACGTGTTAAAAGCAAGTTACATTTTCTCTTTTAGGAAATTAACCA127431.5105912086270725No Hit
CATGGTAGTAGTATCAGTAGTAAAAGTATTTATTCCCATATGTCATTTTC115761.3722517327997326No Hit
AACAGGTAGAAATAGCCCATGTCATTTAAAATTAGCACCCTGAGAAGCTC114031.3517438242152169No Hit
GATACTGGTTCTACTGTTTCTAAGTATGTGATTAAAATGTACATTGTTCT101541.203683836804465No Hit
CCTCTTCTTTATTTTCAGAGTGTCTTTTCTTTTTTGCTACTAGAGTAAAA100441.1906441261437901No Hit
AAAGGTTTCAGTGAGGTGAACAGTTTAAAGGCCTTGGGAATAAGAAAATC97011.1499839374473224No Hit
TGGGAGCTAGCAGTGTAAACAGAGTACATACATAAAAATTACATTTTAAT93891.1129985763006813No Hit
ATCAAGCTGAGTGACCCTGGGGTCAGCCCCGCTGTGTTAAGCCTGGAGAG92461.0960469524418044No Hit
CAGTGGAGGTTAACATTCATCAAGATTAATAACTGGTGTACTTGATAGGC91881.0891714686389031No Hit
GTTTTCTGTTGATATCTTTGATTACTTAACTTAAAAACAAAATAACTCCT91491.084548298495573No Hit
GTCACTATTGGTAACAGAAAAGCTGCACTTTAGGATAACAAAGTCATACG91131.0802807568248067No Hit
CCCAATAGACCCACCCCAATCTCCCCAGACTGGATGTCAGTCTGCCCTTC87221.0339305125673175No Hit
AGGCAAACAACATTCCATGATGACCAAATATTTACTAAAAAGAATAGATA86451.024802715104845No Hit
AAGAGATGGAATCAGTGATTTCAGATTGTTTGTTTCTTTTTTCTCCAGTT86321.023261658390402No Hit
CAGGAAATTTCTAAATGTGACATGACCTACTTACTGTACCTGGTGACAGA80020.948579679151992No Hit
CAGTAAAACACTAATCCAGCCAATAAAAAAAACAAACCGCTGCACAGCTG78090.9257009140837174No Hit
TGTTGGGTAGTTCCTTATGTAATGTTTTTTGTTTTTTATTAATAGGATCG77570.9195366872259438No Hit
TTCAAGCACAGGGTAGAATATTGGGCTGAGTAACACTTGCCTTCATCATG77550.919299601577568No Hit
ACAGTCATGACCCACAGCAAACAGAACTGTTTTAGATATGCTGGGTATTC76470.9064969765652692No Hit
CAGCACTCTTTAGATAAACAGGTCATAAACAAGGAATTATATCAGACTAA74760.8862261536291293No Hit
CCTATTTCTCCTTCCTAACAGTTTACCAAAGTTGAATCATATATAAGCTT73490.8711712149572594No Hit
AAGGTACAATGATTTCCACTTCTCTTATTTACATTTTCTAATCCCTTTCT72530.859791103835216No Hit
CATATAAGGCAAAGCATTAGGTACTTGGTTTATATATTAAAGATCTTACT71050.842246765855399No Hit
CATTTCTCTTGCTTACATGAACTCTATGTCGTGGCATTCAGATCAGTCAC71020.8418911373828353No Hit
GGATTACGTTTGTGATTTTAAGCAGATTTACTTATTAGGCTCTATAACTA70520.8359639961734376No Hit
GGATTCCATCTTAAATCCATCTTTCTCTAGAACTGAGTTTACAGTATTAA68260.8091733179069605No Hit
GTCCTTTGGTGAAGCTATTTATACATGTATATCTTAGGGTTCTGTTTTTA67080.7951852646527822No Hit
AGCAGTATGTTGAGTTTATGGCAGATTAATCTATCATCTTTTAGAAATTT64580.7655495586057942No Hit
TAGAGATCTCTATCTCTTAATGACATTTCTTTTTCCCTCAGGCTTTCTGT63600.7539323618353748No Hit
TTGCTATTCTCAGATGACTCTGTGTTTTTATAATAAAATAAACTGTACTT62240.7378105377458134No Hit
ATCATGTGTGATTTTGTAGTTCTGTTAAAGTTCATGGCTTTTGTGTTTTA62030.7353211384378664No Hit
GCCTCCCAAAGCATTATGAATATGGGCATGAGCCACTTCACCCAACCAAA55450.657319960122194No Hit
TGGTGAAGTTTCATTTCATGAGGAAAAATTCACAAACAACAACCTTCAAA54120.6415537645051963No Hit
TGAATGAAGAAGCAAATTCAAAACAGTAAACATTTCCTTTATACCTTTTT49970.5923584924671962No Hit
AGATACGTTGACAACATTGGTGTGTAACAAAATCCGTATTTATAATGTGT49690.5890392933899335No Hit
GGTGAGCAGTATTTTAAGAAGGTCCTGTTGTCAGTTTTTCAGATTTTCTT48780.5782518963888299No Hit
AAATGACTTAGTTCTGGTTGAGATGAAAGGATTCCACTGAAAGTTTTCTG48420.5739843547180636No Hit
TTCTTTCAGCATACCACTTCATAACTGTTCAGTTTGTACAGTTTGTTCCC48120.5704280699924251No Hit
CTCAAGTTTTCAGAAAAGAAGCCATGACATTAAATGTTAAAATACAGTAA47200.5595221301671336No Hit
TGCCAAACAACAAAGTGCTCAATCTACTATATGTACAAGAAGATTACCAA46120.5467195051548347No Hit
CTAAAGGCTGAATGAAAGGGTAATTCATATACTGAAGATCACACCCAAGC45570.5401996498244973No Hit
TAAATAGTTGCCATTCCAAGTGTCTTATTTTTGTTCAAATTTATGTTTTT45090.5345095942634757No Hit
CATCTTTATTGCCCCTATATCTGTCATATTTTTATATAAAAATGTGTATA45070.5342725086150998No Hit
GTATCTTTGACAATTACCTGATGAAATTAAAGTTTTTTTGTATTCTATAA44680.5296493384717696No Hit
AATAACTGATGTGTTCTGTTAAGCTTATAAAGTTGAACTTTTTTTTTTTT44400.526330139394507No Hit
CAGTTGTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAATCAAGTCA43250.5126977146128925No Hit
AAACAAAAGTGTTGTCTTCATGCTAGTTTAAACTAATTTTTAAAAAATTA42950.509141429887254No Hit
CATCAAATGCTCTTTAATGGCCTTTTAAAATTAAAAGGTATTTAATCTGT42900.5085487157663142No Hit
GCTCAAAAAAAAAAAAAAGAAAAAAGTGGATTTATTTTTATTTTACAGGT39950.4735785826308683No Hit
GAGATACACAGTCTACCTGGTAAGAAAAAAAATAAAGTCTTCCTTATAAA39400.467058727300531No Hit
GTGTATTACCTTAATTTGAGTGATTCTTTAGATGTATTTAGTATTTGTAA39000.46231701433301287No Hit
AAACTTGCATTCGTATCCACAGATAGCAAAAGAAAAATAGGACCACAAAA38950.46172430021207306No Hit
AAGACCAAGTCACTCTTTCTATGCAAAAACACTCACTCAGTTAACTGGTG38810.4600647006734418No Hit
CAGGAGCTTCCAAATAGTATGTTCTCATTAAAAGAGGTGTTCTTGTGACA38100.4516481601560972No Hit
GTGTATTTATTGTAGCCGAGTATCTAATTAAACAAGTTTTTACTAAATCT37640.4461951902434514No Hit
AAACAGCAGCATGCTAATGAACTTAAATTACCTGTGAAGAATTGGAGGCA36990.4384899066712345No Hit
TCCTATATGTGATCCGCAGTTGACTGAAATGTCACGCAGCGCATGACTGT35680.42296079670261283No Hit
GGAAGAACAGGATAGAAAGACTGCTTATATATTGGTCTAAATATGTTATT35110.4162038557238995No Hit
CCTCCAATAGCTTGCTTTTCACAATTGTCCTTTGTTTTGTTATAGTCCTG32840.38929463463323444No Hit
TCTTGTAGGAGTTAGGCCTTGAAAGAGAGATTTAATTGTTTTATTTGTTT32260.3824191508303332No Hit
CATCAGATAAAATCCAAGAGCTTCTTCATTTAACGTAACTCACCATATGC31300.37103903970828983No Hit
GCCAATATTTAACCAATTTTGACCTACATAAAAATACTATTTCAAATGGT30150.35740661492667536No Hit
GTCAAAAAATCTGGAGTTCAGTTGGGATTTTATAATTGATTGTTAAACAT29470.3493457028818946No Hit
GGAAGCTTCTAATAAAATACTCATCCATATATACCTTTTTCACAAGGTGA29190.3460265038046319No Hit
AAAAACTCACGCGACAGTAATCTGTTAAGCCATTTATTTATAATTTATAA28810.34152187648548976No Hit
CACATGGCATCTGTACAGTGTCTATAACAAAATAACCCAAAGTATGAGAT28690.3400993625952343No Hit
GAATTTGTTCTTACAAAAGATAGAGTATACTAAATTATTTATGAAATATA28630.33938810565010663No Hit
ATATATGGCTGTTGTGCCCTTCTCTTAGTGTTAATGAGTGCTTTTTATTT28450.3372543348147235No Hit
CAGCCTTGAACCGATTTTAGATGGCATCTTAATAACACAAGATTTTAAAA26550.3147311982190126No Hit
CTATTTCATATTTAACCACAGTTCTTTTCCCGTAGGCTGATCCTTATTCA26420.3131901415045692No Hit
GAATACATTTCATTCAAATTTATCCGAAACTTTATTAGTATAAATTCTCC25990.30809280006448725No Hit
TATGTAAGACATTCTACTGCCATCTGCAGCATTCCAAATACTTCATGAAT25200.2987279169536391No Hit
TAAGCAGTCACTACCATTGTATTCTATATCAACATGCTTTTATTTTGATA24920.29540871787637646No Hit
ACGCATCTTCTCTCCCTTCCTGTGTCTTCTACTTTTTGTGCTATTTTCAC24850.29457891810706077No Hit
CAAGTTACACTCTAGATCCTAAACGTAAGAAGCAACACTCATTACATTAC24750.2933934898651812No Hit
ATGAGGCCTCTTATACTGCCAAATCAATATAAAGAAGTAAAAGAAATCCC24410.2893630338427909No Hit
ACTATTCCTGCTTGACCTTCAATGCTGTTCCTCAGTTTGTCACTAAAATC24250.28746634865578363No Hit
TCCTTTTAGTTTGTTAATGTGATGGAATAGTTTTCAAATTATCCTTTTTT22380.2652988405326366No Hit
GTTTTATGTATGATCTCTTACCTATGACTCTACTGAAATAGAATTTCTAT22090.261861098631186No Hit
CTAAGTTTCAATATATAAATACGAATAAATCGAATAAATAGCCTTAAAAC21510.2549856148282848No Hit
TACCCTTATTGAGACAATGCCAACATTAATTTTAAAAACCAAAAATTCTA20680.24514656042068478No Hit
CTCCCAAATTGCTGAGATTACAGATGTGAGCCACTGTGCCCAGCCTGATT20530.24336841805786552No Hit
CAGTTGTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAATCAAGTCAA20340.24111610439829442No Hit
GTGGTGGTATGTTCTAAGCTTTCTAATTTTTTTAATGTGACTATTTAGAA18980.22499428030873292No Hit
TCTACCCACTGCAGTATCTAGACAGTAATACACATTTTAATGTTAAGCAA17860.2117174839996823No Hit
CTGGGGCTGTCATATAGCGCTGATCCCCCCAGTTGTGCATTTACAATGGA17530.20780557080147988No Hit
GCAGTTTTAAAATCCTTTTTCTGTATGGGATTATGGAATATTTAAGTTAA17390.20614597126284856No Hit
ACTATGAAAATGATCAGGGATATGTGAGTGTGTGTGTGTCTGTGTGTGTT16650.19737380227294013No Hit
AGTTGGGTACAGTCATGGTAATGCATTATATTTTAAGATTTTGCCTTTCT16620.19701817380037623No Hit
CCTTATAGCATAGTGGGAGACAGACACATAAACAAGAAGGAAGAAGGTGT16080.19061686129422686No Hit
CTAGTTCTGTTATGATGGATCAATGTTTTATTTTAAGGTAGAATTTTTAG15900.1884830904588437No Hit
GTATTTCCATTTCTTAGAGGGAATGGTATATATACTTTATATAATATATA12520.14841561588331592No Hit
CCAGCCAATAAAAAAAACAAACCGCTGCACAGCTGGCATCCAACTTCTTG11270.13359776285982192No Hit
CAGGATATGCCACCTTTAACTCAGTTAACTGAACTTTTGTTTTTTAATAT10780.12778916447461228No Hit
GAAGTATCATTCTCCATGAATGTCATATTGAGATTACATATAAGGTATCA9980.1183057385395761No Hit
CACACCTCTTTCTCTCTATATATGCATATATACATATACATATATATACC9470.11226005450599057No Hit
TTTGAGCTGTCTTGACGTTCACAGATATAAAATATTAAATATATTTTAAT9220.10929648390129176No Hit
CCCTATATCTGTCATATTTTTATATAAAAATGTGTATATTAGTTTAATTG9040.10716271306590863No Hit
TTGTGCTAATAGAGGAGCACTGTCTTAAAATAACTTACTTGCTTAGATGT8720.10336934269189416No Hit
CATCACTAAAACTCTAAGGGCTAAGCCAGAGAAGGGAAGGCTCACCTTAA8440.1000501436146315No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)