FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001795591

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001795591
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences935095
Sequences flagged as poor quality0
Sequence length32-301
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCAAGCACAGGGTAGAATATTGGGCTGAGTAACACTTGCCTTCATCATG139041.4869077473411791No Hit
CAGGAAATTTCTAAATGTGACATGACCTACTTACTGTACCTGGTGACAGA127561.3641394724600175No Hit
AAGAGATGGAATCAGTGATTTCAGATTGTTTGTTTCTTTTTTCTCCAGTT113841.21741641223619No Hit
TGGGAGCTAGCAGTGTAAACAGAGTACATACATAAAAATTACATTTTAAT112901.2073639576727497No Hit
CTAAAATGCCCTCCCCTCGAACCCTTACCAAACTCCTATGCATACTACAG111951.1972045621033158No Hit
AGGCAAACAACATTCCATGATGACCAAATATTTACTAAAAAGAATAGATA105731.1306872563750208No Hit
CAGCACTCTTTAGATAAACAGGTCATAAACAAGGAATTATATCAGACTAA105661.1299386693330624No Hit
CCTCTTCTTTATTTTCAGAGTGTCTTTTCTTTTTTGCTACTAGAGTAAAA104581.1183890406857058No Hit
GTCACTATTGGTAACAGAAAAGCTGCACTTTAGGATAACAAAGTCATACG100461.0743293462161598No Hit
AAACAGCAGCATGCTAATGAACTTAAATTACCTGTGAAGAATTGGAGGCA97881.0467385666696967No Hit
ATCATGTGTGATTTTGTAGTTCTGTTAAAGTTCATGGCTTTTGTGTTTTA95571.0220351942850727No Hit
AGTGCAGTTCAGTCTCCCAACCCCGCCTCTCCCTGCTGCCAACCAGGCAC94701.012731326763591No Hit
CCTCCAATAGCTTGCTTTTCACAATTGTCCTTTGTTTTGTTATAGTCCTG93220.9969040578764724No Hit
AAGGTACAATGATTTCCACTTCTCTTATTTACATTTTCTAATCCCTTTCT92180.985782193253092No Hit
CATGGTAGTAGTATCAGTAGTAAAAGTATTTATTCCCATATGTCATTTTC85640.9158427753329876No Hit
CTCAAGTTTTCAGAAAAGAAGCCATGACATTAAATGTTAAAATACAGTAA84090.8992669194039108No Hit
ACAGTCATGACCCACAGCAAACAGAACTGTTTTAGATATGCTGGGTATTC83320.8910324619423695No Hit
CAGTGGAGGTTAACATTCATCAAGATTAATAACTGGTGTACTTGATAGGC79670.8519989947545437No Hit
GCCTCCCAAAGCATTATGAATATGGGCATGAGCCACTTCACCCAACCAAA75740.8099711793988846No Hit
AGATACGTTGACAACATTGGTGTGTAACAAAATCCGTATTTATAATGTGT74420.7958549666076709No Hit
AAACAAAAGTGTTGTCTTCATGCTAGTTTAAACTAATTTTTAAAAAATTA73290.783770632930344No Hit
AAATGACTTAGTTCTGGTTGAGATGAAAGGATTCCACTGAAAGTTTTCTG72370.773932060378892No Hit
ATCAAGCTGAGTGACCCTGGGGTCAGCCCCGCTGTGTTAAGCCTGGAGAG70940.7586394965217438No Hit
GGATTACGTTTGTGATTTTAAGCAGATTTACTTATTAGGCTCTATAACTA70720.7562867943898748No Hit
AAACTTGCATTCGTATCCACAGATAGCAAAAGAAAAATAGGACCACAAAA70400.7528646821980655No Hit
GAGATACACAGTCTACCTGGTAAGAAAAAAAATAAAGTCTTCCTTATAAA70170.7504050390602025No Hit
CATTTCTCTTGCTTACATGAACTCTATGTCGTGGCATTCAGATCAGTCAC69490.7431330506526075No Hit
TGTTGGGTAGTTCCTTATGTAATGTTTTTTGTTTTTTATTAATAGGATCG66370.709767456782466No Hit
GTCCTTTGGTGAAGCTATTTATACATGTATATCTTAGGGTTCTGTTTTTA65620.7017468813329127No Hit
CAAGTTACACTCTAGATCCTAAACGTAAGAAGCAACACTCATTACATTAC65300.6983247691411033No Hit
GGATTCCATCTTAAATCCATCTTTCTCTAGAACTGAGTTTACAGTATTAA65220.697469241093151No Hit
CCCAATAGACCCACCCCAATCTCCCCAGACTGGATGTCAGTCTGCCCTTC63850.682818323271967No Hit
AGCAGTATGTTGAGTTTATGGCAGATTAATCTATCATCTTTTAGAAATTT63460.6786476240381993No Hit
CCTATTTCTCCTTCCTAACAGTTTACCAAAGTTGAATCATATATAAGCTT62310.6663494083488843No Hit
TTGCTATTCTCAGATGACTCTGTGTTTTTATAATAAAATAAACTGTACTT59560.6369406317005224No Hit
TGCCAAACAACAAAGTGCTCAATCTACTATATGTACAAGAAGATTACCAA59350.6346948705746475No Hit
AATAACTGATGTGTTCTGTTAAGCTTATAAAGTTGAACTTTTTTTTTTTT59300.6341601655446772No Hit
GGTGAGCAGTATTTTAAGAAGGTCCTGTTGTCAGTTTTTCAGATTTTCTT58510.6257118260711478No Hit
CATATAAGGCAAAGCATTAGGTACTTGGTTTATATATTAAAGATCTTACT58290.6233591239392788No Hit
CTAAAGGCTGAATGAAAGGGTAATTCATATACTGAAGATCACACCCAAGC57030.6098845571840295No Hit
GAATTTGTTCTTACAAAAGATAGAGTATACTAAATTATTTATGAAATATA56880.6082804420941188No Hit
GAATACATTTCATTCAAATTTATCCGAAACTTTATTAGTATAAATTCTCC55950.5983349285366727No Hit
TTCTTTCAGCATACCACTTCATAACTGTTCAGTTTGTACAGTTTGTTCCC55770.59640999042878No Hit
TAGAGATCTCTATCTCTTAATGACATTTCTTTTTCCCTCAGGCTTTCTGT54850.5865714178773279No Hit
GTTTTCTGTTGATATCTTTGATTACTTAACTTAAAAACAAAATAACTCCT54460.5824007186435604No Hit
TCCTATATGTGATCCGCAGTTGACTGAAATGTCACGCAGCGCATGACTGT54110.5786577834337687No Hit
CAGGAGCTTCCAAATAGTATGTTCTCATTAAAAGAGGTGTTCTTGTGACA53900.5764120223078938No Hit
CAGTAAAACACTAATCCAGCCAATAAAAAAAACAAACCGCTGCACAGCTG53280.5697816799362632No Hit
TGAATGAAGAAGCAAATTCAAAACAGTAAACATTTCCTTTATACCTTTTT49910.5337425609162705No Hit
CCCCCACTTTGACAGAAGGGCAGACTGACATCCAGTCTGGGGAGATTGGG49890.5335286789042825No Hit
CAAACCACGCTCCTTCCACTGGGCCCAATATGACATCACAGCCCTCCCCA49290.5271122185446399No Hit
CCTTATAGCATAGTGGGAGACAGACACATAAACAAGAAGGAAGAAGGTGT48520.5188777610830985No Hit
GCTCAAAAAAAAAAAAAAGAAAAAAGTGGATTTATTTTTATTTTACAGGT46890.5014463771060694No Hit
AAAAACTCACGCGACAGTAATCTGTTAAGCCATTTATTTATAATTTATAA46410.4963132088183554No Hit
CATCTTTATTGCCCCTATATCTGTCATATTTTTATATAAAAATGTGTATA45230.48369417011105825No Hit
GGAAGCTTCTAATAAAATACTCATCCATATATACCTTTTTCACAAGGTGA40830.43664012747367914No Hit
CTCCCAAATTGCTGAGATTACAGATGTGAGCCACTGTGCCCAGCCTGATT39280.42006427154460246No Hit
CCAATACGTGTTAAAAGCAAGTTACATTTTCTCTTTTAGGAAATTAACCA39260.41985038953261433No Hit
GTCAAAAAATCTGGAGTTCAGTTGGGATTTTATAATTGATTGTTAAACAT39250.4197434485266203No Hit
GTTTTATGTATGATCTCTTACCTATGACTCTACTGAAATAGAATTTCTAT38880.4157866313048407No Hit
TCTTGTAGGAGTTAGGCCTTGAAAGAGAGATTTAATTGTTTTATTTGTTT38320.40979793496917427No Hit
CTATTTCATATTTAACCACAGTTCTTTTCCCGTAGGCTGATCCTTATTCA38220.40872852490923384No Hit
ATGAGGCCTCTTATACTGCCAAATCAATATAAAGAAGTAAAAGAAATCCC37810.4043439436634781No Hit
AACAGGTAGAAATAGCCCATGTCATTTAAAATTAGCACCCTGAGAAGCTC37390.39985242141172816No Hit
AAAGGTTTCAGTGAGGTGAACAGTTTAAAGGCCTTGGGAATAAGAAAATC34270.3664868275415867No Hit
AAGACCAAGTCACTCTTTCTATGCAAAAACACTCACTCAGTTAACTGGTG33660.35996342617595No Hit
TATGTAAGACATTCTACTGCCATCTGCAGCATTCCAAATACTTCATGAAT33440.35761072404408106No Hit
CAGTTGTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAATCAAGTCA33180.35483025788823597No Hit
TGGTGAAGTTTCATTTCATGAGGAAAAATTCACAAACAACAACCTTCAAA30360.32467289419791573No Hit
GTGTATTACCTTAATTTGAGTGATTCTTTAGATGTATTTAGTATTTGTAA27560.29472941251958357No Hit
GATACTGGTTCTACTGTTTCTAAGTATGTGATTAAAATGTACATTGTTCT27560.29472941251958357No Hit
CTGGGGCTGTCATATAGCGCTGATCCCCCCAGTTGTGCATTTACAATGGA26840.28702966008801245No Hit
TCCTTTTAGTTTGTTAATGTGATGGAATAGTTTTCAAATTATCCTTTTTT26630.2847838989621375No Hit
GAAGTATCATTCTCCATGAATGTCATATTGAGATTACATATAAGGTATCA26010.27815355659050683No Hit
TAAATAGTTGCCATTCCAAGTGTCTTATTTTTGTTCAAATTTATGTTTTT24990.2672455739791144No Hit
ACTATGAAAATGATCAGGGATATGTGAGTGTGTGTGTGTCTGTGTGTGTT24740.26457204882926333No Hit
GCCAATATTTAACCAATTTTGACCTACATAAAAATACTATTTCAAATGGT23830.2548404172838054No Hit
CATCAGATAAAATCCAAGAGCTTCTTCATTTAACGTAACTCACCATATGC23180.24788925189419259No Hit
CATCACTAAAACTCTAAGGGCTAAGCCAGAGAAGGGAAGGCTCACCTTAA21280.22757046075532433No Hit
CTAGTTCTGTTATGATGGATCAATGTTTTATTTTAAGGTAGAATTTTTAG20750.22190258743764002No Hit
GCAGTTTTAAAATCCTTTTTCTGTATGGGATTATGGAATATTTAAGTTAA20370.21783882920986636No Hit
CATCAAATGCTCTTTAATGGCCTTTTAAAATTAAAAGGTATTTAATCTGT20250.21655553713793788No Hit
ATATATGGCTGTTGTGCCCTTCTCTTAGTGTTAATGAGTGCTTTTTATTT19430.2077863746464263No Hit
CTAAGTTTCAATATATAAATACGAATAAATCGAATAAATAGCCTTAAAAC19390.20735861062245012No Hit
GTATCTTTGACAATTACCTGATGAAATTAAAGTTTTTTTGTATTCTATAA19140.20468508547259903No Hit
GTGGTGGTATGTTCTAAGCTTTCTAATTTTTTTAATGTGACTATTTAGAA18920.20233238334073006No Hit
TAAGTGCAAATAGTGTATCTGACCTATTATCAATCATGTTTATACTTTTA18160.1942048668851828No Hit
TAAGCAGTCACTACCATTGTATTCTATATCAACATGCTTTTATTTTGATA17070.18254829723183205No Hit
CAGTTGTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAATCAAGTCAA16960.18137194616589758No Hit
ACGCATCTTCTCTCCCTTCCTGTGTCTTCTACTTTTTGTGCTATTTTCAC16540.17688042391414777No Hit
ACTATTCCTGCTTGACCTTCAATGCTGTTCCTCAGTTTGTCACTAAAATC16200.17324442971035028No Hit
GTATTTCCATTTCTTAGAGGGAATGGTATATATACTTTATATAATATATA16180.1730305476983622No Hit
CACATGGCATCTGTACAGTGTCTATAACAAAATAACCCAAAGTATGAGAT15540.16618632331474342No Hit
GGAAGCAAAGATAAATGTTAAGACTTACACACAAAAGTAATATCACAACA15380.1644752672188387No Hit
TTGTGCTAATAGAGGAGCACTGTCTTAAAATAACTTACTTGCTTAGATGT13690.1464022372058454No Hit
TACCCTTATTGAGACAATGCCAACATTAATTTTAAAAACCAAAAATTCTA13520.14458424010394666No Hit
GTGAGGGGACTTGCTAAGTATTGTTAACTAATTTTAAATTAAACAAATTT13300.14223153797207772No Hit
CACACCTCTTTCTCTCTATATATGCATATATACATATACATATATATACC13240.14158989193611343No Hit
TGTAGTTCTGTTAAAGTTCATGGCTTTTGTGTTTTACCTTAATTATTCTA11980.128115325180864No Hit
TTTGAGCTGTCTTGACGTTCACAGATATAAAATATTAAATATATTTTAAT11830.1265112100909533No Hit
CAGGATATGCCACCTTTAACTCAGTTAACTGAACTTTTGTTTTTTAATAT11520.12319603890513799No Hit
CATGTGTGATTTTGTAGTTCTGTTAAAGTTCATGGCTTTTGTGTTTTACC11370.12159192381522732No Hit
GAATGTTGTTTCTAGGTCCTACTCTAAATAATATTAACAAGCATTTAAAT11270.12052251375528689No Hit
TTGAAATAGACATTGAAGGTGTCAACCAATAAACTTCTTTAAAAACTTGT11030.11795592961142987No Hit
GGGTGACAGAGAAAGATCCTATCTCAAAAAACAAAACAAGAAGAAGAAAA10630.11367828937166811No Hit
CCGCAGTTGACTGAAATGTCACGCAGCGCATGACTGTAGTTTACCTAGAA10220.10929370812591234No Hit
GTATGTCCAAGATCAAAGTACACTGTAAAAAGCAATACTAAACTATAATT10090.10790347504798978No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)