FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001795595

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001795595
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences868654
Sequences flagged as poor quality0
Sequence length32-301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAAGGAGAGTGTCTGTCGCTCAGTCCCACTCAGGGGCCACTCTTCTTTG212932.4512636792094438No Hit
TCCCTCATCCTCTCCCCATAGAAAAGTCCAACCTGATCGTGGTCCAGAGA201012.314039882392759No Hit
ATTTCCCAGGGCCTCTTACCCACTCCAGGCTGTCAGCAGGGATCTTGTGA174882.0132296633642395No Hit
ACATAGAAAGCCAGGGTCCCAGATCAGCCACTCATTCCAGGGGAGGAAGG172381.984449504635908No Hit
ACCGGCATTGATTTTGGGACCCACATGCCCGAGGAATACACTCACAGGCT157591.8141860855990992No Hit
GAAGAACTTGAGGCTCGTGGGCGCTCAGCCTTCTCTTGTGTCTTTTTAGA155701.7924282856004807No Hit
GGAATGTTCTATTATTAAACTCATCATGTATACTTACAGGGCTTTTGGAA123491.4216247205446588No Hit
CTCAAGTTTTCAGAAAAGAAGCCATGACATTAAATGTTAAAATACAGTAA117221.3494440824540035No Hit
GGCTACACTGGACACCTCATGGCTGTATGGCCTGGTCCAAGCCAGTGTCC87731.009953330094606No Hit
CAGTGGAGGTTAACATTCATCAAGATTAATAACTGGTGTACTTGATAGGC87231.00419729834894No Hit
GTTTTATGTATGATCTCTTACCTATGACTCTACTGAAATAGAATTTCTAT86040.9904979427942541No Hit
GGTGAGCAGTATTTTAAGAAGGTCCTGTTGTCAGTTTTTCAGATTTTCTT85280.9817487745408413No Hit
TTTTCATTTTGGAAGTTCACTGGTCTATGAACAAAACTTTTTAAAACGAT84680.9748415364460419No Hit
AAACTTGCATTCGTATCCACAGATAGCAAAAGAAAAATAGGACCACAAAA83470.9609119396215294No Hit
GTCCTTTGGTGAAGCTATTTATACATGTATATCTTAGGGTTCTGTTTTTA83180.957573441209043No Hit
ACAGTCATGACCCACAGCAAACAGAACTGTTTTAGATATGCTGGGTATTC81450.9376575713690376No Hit
CACAGACCAGGTTCCAGACATGGCTATTTTTATGCCTACAGATATCATGG79260.9124461523230192No Hit
GTACACCACACACACACACGCACACGCACACGCAGAGCTGAGGGAAGGCT77360.8905732316894874No Hit
CTAAAGGCTGAATGAAAGGGTAATTCATATACTGAAGATCACACCCAAGC76280.8781402031188483No Hit
AGCTCTTTCTCCCTTTGCCTACTTCTCCCTCTAGTACAGAATACGCCATC75230.866052536452949No Hit
CACATGGCATCTGTACAGTGTCTATAACAAAATAACCCAAAGTATGAGAT73790.8494751650254302No Hit
CAGGAGCTTCCAAATAGTATGTTCTCATTAAAAGAGGTGTTCTTGTGACA72450.8340489999470445No Hit
GAGATACACAGTCTACCTGGTAAGAAAAAAAATAAAGTCTTCCTTATAAA71950.8282929682013782No Hit
GACTTCTGAATGAATTTATTTCAGAGTAATTTTCCAGAACTTACTGGTTG71300.820810126932012No Hit
GGAGGCTGGCCTGAGACATTCCTATGTCCTGCTCGCCCACAGGCCTTCTT69720.8026210666157065No Hit
CCTGGTCTTTGAGGGGAACAACTTATATCTTACGTTTCATCTTATGTTTC68040.7832807999502679No Hit
AAACAGCAGCATGCTAATGAACTTAAATTACCTGTGAAGAATTGGAGGCA66970.770962892014542No Hit
AGATACGTTGACAACATTGGTGTGTAACAAAATCCGTATTTATAATGTGT65920.7588752253486428No Hit
TTCAAGCACAGGGTAGAATATTGGGCTGAGTAACACTTGCCTTCATCATG62110.7150142634466659No Hit
TCCACAGCACCCAAGCTCCCCAGCCAGGCCACCCACCCCTTTGAAAGAGA62000.7137479364626191No Hit
GAAGTATCATTCTCCATGAATGTCATATTGAGATTACATATAAGGTATCA61880.7123664888436593No Hit
CCTCCAATAGCTTGCTTTTCACAATTGTCCTTTGTTTTGTTATAGTCCTG60710.6988973745588002No Hit
CACTCAAATCCTTCTAACAATACTTTATATATTTTAAATACCATTTTGAA60260.6937169459877005No Hit
AATAACTGATGTGTTCTGTTAAGCTTATAAAGTTGAACTTTTTTTTTTTT59870.6892272412260808No Hit
ACTCTGGTTTCTGGTGGGACCATTATGGACATCAGGACATTCTCACCAAG58630.6749522824968285No Hit
CTCCCAAATTGCTGAGATTACAGATGTGAGCCACTGTGCCCAGCCTGATT55380.6375380761499976No Hit
AGGGGAGGAGTTCAGAGGAAGACACTTGCCAGGGAAATATCCCTGTGGCT54720.6299401142457182No Hit
GTCAGTCAGCGTGTTTTGGTCGATCTGGGTCTCAGCACATTACTGATGGG53300.6135929840880259No Hit
ATGAGGCCTCTTATACTGCCAAATCAATATAAAGAAGTAAAAGAAATCCC52300.6020809205966933No Hit
CAGCACTCTTTAGATAAACAGGTCATAAACAAGGAATTATATCAGACTAA52300.6020809205966933No Hit
CAGGGAGGAAAGGAGGACAGCCAACAAGTACCAGGCAGCATGGTCAACTG51560.5935619936131071No Hit
CATCAGATAAAATCCAAGAGCTTCTTCATTTAACGTAACTCACCATATGC50370.5798626380584214No Hit
GGAAGAACAGGATAGAAAGACTGCTTATATATTGGTCTAAATATGTTATT48080.5535000126632699No Hit
ATCATGTGTGATTTTGTAGTTCTGTTAAAGTTCATGGCTTTTGTGTTTTA46330.5333539015534379No Hit
AAACAAAAGTGTTGTCTTCATGCTAGTTTAAACTAATTTTTAAAAAATTA45770.5269071459982917No Hit
CAAGTTACACTCTAGATCCTAAACGTAAGAAGCAACACTCATTACATTAC44120.5079122412375928No Hit
TCCTATATGTGATCCGCAGTTGACTGAAATGTCACGCAGCGCATGACTGT43050.495594333301867No Hit
TCTTCATGCAGCTTCCCCTAATGTTAATATCTTACATACCCATGCCTGAC41550.4783262380648682No Hit
AAGGTACAATGATTTCCACTTCTCTTATTTACATTTTCTAATCCCTTTCT40740.4690014666368888No Hit
TCACTTCAGGGCACAGAGAGGTCACCCAGCCTTCTGCTTCTTCCCTCAGG40520.46646881266879564No Hit
TTGAAATAGACATTGAAGGTGTCAACCAATAAACTTCTTTAAAAACTTGT38260.440451549178384No Hit
TTTGAGCTGTCTTGACGTTCACAGATATAAAATATTAAATATATTTTAAT37690.4338896729883245No Hit
GCCAATATTTAACCAATTTTGACCTACATAAAAATACTATTTCAAATGGT36240.4171971809258922No Hit
GTGTATTTATTGTAGCCGAGTATCTAATTAAACAAGTTTTTACTAAATCT35850.4127074761642725No Hit
ATATATGGCTGTTGTGCCCTTCTCTTAGTGTTAATGAGTGCTTTTTATTT35270.4060304793392996No Hit
CCTTATAGCATAGTGGGAGACAGACACATAAACAAGAAGGAAGAAGGTGT35160.40476415235525304No Hit
CATTCAAGATAGAGAAAACACTGTCTGCCGAGAATAATTGTTTTTATTTC35090.40395830791085974No Hit
GGATTCCATCTTAAATCCATCTTTCTCTAGAACTGAGTTTACAGTATTAA35070.4037280666410331No Hit
ATCTTGGGTGCTGTGCTCAGACTCCCCCAATCTCTTCCACAGAAATCTCC34350.3954393809272737No Hit
AGCAGTATGTTGAGTTTATGGCAGATTAATCTATCATCTTTTAGAAATTT34120.39279160632426713No Hit
GCCTCCCAAAGCATTATGAATATGGGCATGAGCCACTTCACCCAACCAAA33930.390604314260914No Hit
CTAAAATGCCCTCCCCTCGAACCCTTACCAAACTCCTATGCATACTACAG33400.3845029206105078No Hit
GTTTTCTGTTGATATCTTTGATTACTTAACTTAAAAACAAAATAACTCCT33110.3811644221980213No Hit
GGTGAGCACTGAGGGAATGAAAGTGGGATCAGGGATCCACTTCCTTGCCC31970.3680406698179022No Hit
ATATTTCAACAAAAAAATTATGCCTATTAGAATCAAAATATGATAGCAAA31720.3651626539450691No Hit
CATTTTCCTGTGTCTGGCCCCCTTAGGAGGACAAAGGCCTGCCCCATGGC31610.3638963269610225No Hit
TGCCAAACAACAAAGTGCTCAATCTACTATATGTACAAGAAGATTACCAA30310.3489306444222901No Hit
TAAGCAGTCACTACCATTGTATTCTATATCAACATGCTTTTATTTTGATA29940.34467118093049703No Hit
AAAGGTTTCAGTGAGGTGAACAGTTTAAAGGCCTTGGGAATAAGAAAATC29660.34144780315292395No Hit
TTGCTATTCTCAGATGACTCTGTGTTTTTATAATAAAATAAACTGTACTT29450.3390302698197441No Hit
AGTTGGGTACAGTCATGGTAATGCATTATATTTTAAGATTTTGCCTTTCT28160.3241797079159251No Hit
CATGGTAGTAGTATCAGTAGTAAAAGTATTTATTCCCATATGTCATTTTC27650.3183085555353455No Hit
TTTCCCCAAACACAGCCTTCCTTCCCCCAGATCCCCAAGCTGCTCCATCG26590.306105768234533No Hit
AAGAGATGGAATCAGTGATTTCAGATTGTTTGTTTCTTTTTTCTCCAGTT25470.2932122571242405No Hit
GGAGTTTCACCATATTGGCCAGGCTGTTCTCAAACTCCTGACCTTGTGAT24600.28319676188678117No Hit
AGGCAAACAACATTCCATGATGACCAAATATTTACTAAAAAGAATAGATA24190.2784768158553348No Hit
GTCAAAAAATCTGGAGTTCAGTTGGGATTTTATAATTGATTGTTAAACAT23980.27605928252215495No Hit
CCTATTTCTCCTTCCTAACAGTTTACCAAAGTTGAATCATATATAAGCTT23870.27479295553810834No Hit
AGTGCAGTTCAGTCTCCCAACCCCGCCTCTCCCTGCTGCCAACCAGGCAC23740.27329638728423516No Hit
GGAAGCTTCTAATAAAATACTCATCCATATATACCTTTTTCACAAGGTGA23660.27237542220492855No Hit
GATACTGGTTCTACTGTTTCTAAGTATGTGATTAAAATGTACATTGTTCT23090.265813546014869No Hit
TACCCTTATTGAGACAATGCCAACATTAATTTTAAAAACCAAAAATTCTA22710.2614389618881626No Hit
AAATGACTTAGTTCTGGTTGAGATGAAAGGATTCCACTGAAAGTTTTCTG21600.24866057141278344No Hit
CATATAAGGCAAAGCATTAGGTACTTGGTTTATATATTAAAGATCTTACT21500.2475093650636502No Hit
ACTATTCCTGCTTGACCTTCAATGCTGTTCCTCAGTTTGTCACTAAAATC21230.2444011079209904No Hit
TGAATGAAGAAGCAAATTCAAAACAGTAAACATTTCCTTTATACCTTTTT20510.23611242220723094No Hit
TTCTTTCAGCATACCACTTCATAACTGTTCAGTTTGTACAGTTTGTTCCC20350.23427049204861775No Hit
CATTTCTCTTGCTTACATGAACTCTATGTCGTGGCATTCAGATCAGTCAC19380.22310379046202514No Hit
CATCTTTATTGCCCCTATATCTGTCATATTTTTATATAAAAATGTGTATA19150.22045601585901864No Hit
CATATGATAACAGCAAATACATGTTACTTAAAATTACCATTTCTCTCATT18930.2179233618909255No Hit
AAAAACTCACGCGACAGTAATCTGTTAAGCCATTTATTTATAATTTATAA18740.2157360698275723No Hit
CTCCCTACAGAGTCAGCCACTTAAGCTGATAGAGACATGATGTAACCGTG18100.20836834919311947No Hit
CAGATTTTTGAAAAGTACTACTATGTTCTCTAATAAAAAATAGTAGATGA17930.20641129839959294No Hit
GGATTACGTTTGTGATTTTAAGCAGATTTACTTATTAGGCTCTATAACTA17860.20560545395519966No Hit
CAGTAAAACACTAATCCAGCCAATAAAAAAAACAAACCGCTGCACAGCTG17750.20433912697115308No Hit
GTGAGGGGACTTGCTAAGTATTGTTAACTAATTTTAAATTAAACAAATTT16800.19340266665438713No Hit
CAGTTGTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAATCAAGTCA16410.18891296189276743No Hit
TGTTGGGTAGTTCCTTATGTAATGTTTTTTGTTTTTTATTAATAGGATCG16290.18753151427380751No Hit
TAGAGATCTCTATCTCTTAATGACATTTCTTTTTCCCTCAGGCTTTCTGT16240.1869559110992409No Hit
CTAAGTAACTATCTTAAGGGTTGCTCCAAAAATCTTACCTTTTCTAGATA16150.18591982538502097No Hit
TTTATAAGCTTAACAGAACACATCAGTTATTTTATAACTTTAAAGTGAAC15950.18361741268675447No Hit
CATCACTAAAACTCTAAGGGCTAAGCCAGAGAAGGGAAGGCTCACCTTAA15460.17797650157600148No Hit
GCTCAAAAAAAAAAAAAAGAAAAAAGTGGATTTATTTTTATTTTACAGGT15400.17728577776652152No Hit
TTGTTTGTTTGTTTGCTTGCTTGTTTTAAGATTGTTCCAGGACACGAAGG15190.1748682444333417No Hit
GGAAGCAAAGATAAATGTTAAGACTTACACACAAAAGTAATATCACAACA15000.1726809523699885No Hit
TAAGTGCAAATAGTGTATCTGACCTATTATCAATCATGTTTATACTTTTA14730.1695726952273287No Hit
CTAAGTTTCAATATATAAATACGAATAAATCGAATAAATAGCCTTAAAAC14440.16623419681484228No Hit
TTACAGTAAGTTTTGTTGGCTTACTTTAAAATTATTTCTCTCCTTATAAT14190.16335618094200913No Hit
TCTTGTAGGAGTTAGGCCTTGAAAGAGAGATTTAATTGTTTTATTTGTTT13620.15679430475194958No Hit
GAATGTTGTTTCTAGGTCCTACTCTAAATAATATTAACAAGCATTTAAAT13490.15529773649807635No Hit
GTGGTGGTATGTTCTAAGCTTTCTAATTTTTTTAATGTGACTATTTAGAA13150.15138363491102327No Hit
CTCCCTACAGAGTCAGCCACTTAAGCTGATAGAGACATGATGTAACCATG13040.15011730792697667No Hit
CCCAATAGACCCACCCCAATCTCCCCAGACTGGATGTCAGTCTGCCCTTC12540.1443612761813104No Hit
TGGTGAAGTTTCATTTCATGAGGAAAAATTCACAAACAACAACCTTCAAA12010.13825988253090415No Hit
CACACCTCTTTCTCTCTATATATGCATATATACATATACATATATATACC11340.13054679999171132No Hit
CAGGATATGCCACCTTTAACTCAGTTAACTGAACTTTTGTTTTTTAATAT11250.1295107142774914No Hit
TATGTAAGACATTCTACTGCCATCTGCAGCATTCCAAATACTTCATGAAT11240.12939559364257805No Hit
ATCAAGCTGAGTGACCCTGGGGTCAGCCCCGCTGTGTTAAGCCTGGAGAG10960.12617221586500493No Hit
CTATTTCATATTTAACCACAGTTCTTTTCCCGTAGGCTGATCCTTATTCA10770.12398492380165174No Hit
CTGAGAGCATCTGGTTGGGAAGGACTGATCTAACAGCGGCTGGCAGCTGG10670.1228337174525185No Hit
CATGAATGTCATATTGAGATTACATATAAGGTATCAATAAATACTCACCA10430.12007082221459867No Hit
TAAATAGTTGCCATTCCAAGTGTCTTATTTTTGTTCAAATTTATGTTTTT10300.11857425396072545No Hit
CAGTTGTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAATCAAGTCAA10110.11638696189737227No Hit
GATTCTGGCCTCTCTCCCTCTCAGGGCAGGGTGCCATTTGCCGTGCCTGA9610.11063093015170598No Hit
CTGGGGCTGTCATATAGCGCTGATCCCCCCAGTTGTGCATTTACAATGGA9600.11051580951679264No Hit
CCTCTTACCCACTCCAGGCTGTCAGCAGGGATCTTGTGAAATGTCATCTG9560.11005532697713934No Hit
TACTACCCAGCTAAAATTATCATCTTTGTAATTAAAGCTATAGCTTAAAA8940.10291784761251314No Hit
TTTGGAAGTTCACTGGTCTATGAACAAAACTTTTTAAAACGATGACTGTA8790.10119103808881326No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)