FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001795597

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001795597
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences893304
Sequences flagged as poor quality0
Sequence length32-301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTCATCCTCTCCCCATAGAAAAGTCCAACCTGATCGTGGTCCAGAGA227042.5415759920475No Hit
ATTTCCCAGGGCCTCTTACCCACTCCAGGCTGTCAGCAGGGATCTTGTGA209262.3425396057781005No Hit
AGAAGGAGAGTGTCTGTCGCTCAGTCCCACTCAGGGGCCACTCTTCTTTG192402.1538020651424374No Hit
GAAGAACTTGAGGCTCGTGGGCGCTCAGCCTTCTCTTGTGTCTTTTTAGA175331.9627137010469No Hit
ACCGGCATTGATTTTGGGACCCACATGCCCGAGGAATACACTCACAGGCT173931.9470415446477345No Hit
ACATAGAAAGCCAGGGTCCCAGATCAGCCACTCATTCCAGGGGAGGAAGG161971.8131565514091508No Hit
GGAATGTTCTATTATTAAACTCATCATGTATACTTACAGGGCTTTTGGAA135831.5205350026418778No Hit
AAACTTGCATTCGTATCCACAGATAGCAAAAGAAAAATAGGACCACAAAA119781.3408649239228752No Hit
ATATTTCAACAAAAAAATTATGCCTATTAGAATCAAAATATGATAGCAAA92951.0405192409302992No Hit
TTTTCATTTTGGAAGTTCACTGGTCTATGAACAAAACTTTTTAAAACGAT89531.0022344017266238No Hit
CCTCCAATAGCTTGCTTTTCACAATTGTCCTTTGTTTTGTTATAGTCCTG87690.9816367104591495No Hit
CTCCCAAATTGCTGAGATTACAGATGTGAGCCACTGTGCCCAGCCTGATT87000.9739125762338464No Hit
CTCAAGTTTTCAGAAAAGAAGCCATGACATTAAATGTTAAAATACAGTAA85460.9566732041947646No Hit
AATAACTGATGTGTTCTGTTAAGCTTATAAAGTTGAACTTTTTTTTTTTT83830.938426336387165No Hit
AAACAGCAGCATGCTAATGAACTTAAATTACCTGTGAAGAATTGGAGGCA83580.9356277370301711No Hit
ATGAGGCCTCTTATACTGCCAAATCAATATAAAGAAGTAAAAGAAATCCC78930.8835737889900863No Hit
CATCAGATAAAATCCAAGAGCTTCTTCATTTAACGTAACTCACCATATGC71080.7956977691804805No Hit
CACATGGCATCTGTACAGTGTCTATAACAAAATAACCCAAAGTATGAGAT70290.7868541952123801No Hit
CAGGAGCTTCCAAATAGTATGTTCTCATTAAAAGAGGTGTTCTTGTGACA69120.773756750221649No Hit
CAGTGGAGGTTAACATTCATCAAGATTAATAACTGGTGTACTTGATAGGC68510.7669281677905841No Hit
AAATGACTTAGTTCTGGTTGAGATGAAAGGATTCCACTGAAAGTTTTCTG68240.7639056804850308No Hit
CAGGGAGGAAAGGAGGACAGCCAACAAGTACCAGGCAGCATGGTCAACTG67830.759315977539561No Hit
ACAGTCATGACCCACAGCAAACAGAACTGTTTTAGATATGCTGGGTATTC65690.735359967043694No Hit
GTTTTCTGTTGATATCTTTGATTACTTAACTTAAAAACAAAATAACTCCT65400.7321135917895811No Hit
GAGATACACAGTCTACCTGGTAAGAAAAAAAATAAAGTCTTCCTTATAAA64990.7275238888441113No Hit
GTTTTATGTATGATCTCTTACCTATGACTCTACTGAAATAGAATTTCTAT63590.711851732444946No Hit
CACAGACCAGGTTCCAGACATGGCTATTTTTATGCCTACAGATATCATGG63070.7060306457823988No Hit
CTGAGAGCATCTGGTTGGGAAGGACTGATCTAACAGCGGCTGGCAGCTGG61180.6848732346435256No Hit
AGATACGTTGACAACATTGGTGTGTAACAAAATCCGTATTTATAATGTGT60920.6819626913122521No Hit
CATTCAAGATAGAGAAAACACTGTCTGCCAAGAATAATTGTTTTTATTTC57300.6414389726229817No Hit
CAGCACTCTTTAGATAAACAGGTCATAAACAAGGAATTATATCAGACTAA55690.6234159927639416No Hit
CCTATTTCTCCTTCCTAACAGTTTACCAAAGTTGAATCATATATAAGCTT53560.5995719262423542No Hit
AGGGGAGGAGTTCAGAGGAAGACACTTGCCAGGGAAATATCCCTGTGGCT53500.5989002623966757No Hit
GGAAGAACAGGATAGAAAGACTGCTTATATATTGGTCTAAATATGTTATT53050.5938627835540868No Hit
TTGAAATAGACATTGAAGGTGTCAACCAATAAACTTCTTTAAAAACTTGT52490.5875939209944208No Hit
ATATATGGCTGTTGTGCCCTTCTCTTAGTGTTAATGAGTGCTTTTTATTT52170.5840117138174686No Hit
GTCCTTTGGTGAAGCTATTTATACATGTATATCTTAGGGTTCTGTTTTTA51600.5776309072835227No Hit
TTTGAGCTGTCTTGACGTTCACAGATATAAAATATTAAATATATTTTAAT51190.573041204338053No Hit
GGAGGCTGGCCTGAGACATTCCTATGTCCTGCTCGCCCACAGGCCTTCTT50640.5668842857526666No Hit
GATTCTGGCCTCTCTCCCTCTCAGGGCAGGGTGCCATTTGCCGTGCCTGA49850.558040711784566No Hit
GAAGTATCATTCTCCATGAATGTCATATTGAGATTACATATAAGGTATCA49650.5558018322989711No Hit
GCTCAAAAAAAAAAAAAAGAAAAAAGTGGATTTATTTTTATTTTACAGGT49600.5552421124275723No Hit
CATATAAGGCAAAGCATTAGGTACTTGGTTTATATATTAAAGATCTTACT48870.5470702023051504No Hit
CATTCAAGATAGAGAAAACACTGTCTGCCGAGAATAATTGTTTTTATTTC46720.5230022478350036No Hit
CAGGAAATTTCTAAATGTGACATGACCTACTTACTGTACCTGGTGACAGA46600.5216589201436466No Hit
ACTCTGGTTTCTGGTGGGACCATTATGGACATCAGGACATTCTCACCAAG46580.521435032195087No Hit
TTCAAGCACAGGGTAGAATATTGGGCTGAGTAACACTTGCCTTCATCATG44790.5013970607990114No Hit
AAGGTACAATGATTTCCACTTCTCTTATTTACATTTTCTAATCCCTTTCT43970.4922176549080716No Hit
AAAGGTTTCAGTGAGGTGAACAGTTTAAAGGCCTTGGGAATAAGAAAATC43550.48751600798832195No Hit
GTCAGTCAGCGTGTTTTGGTCGATCTGGGTCTCAGCACATTACTGATGGG43160.48315019299141165No Hit
TCCACAGCACCCAAGCTCCCCAGCCAGGCCACCCACCCCTTTGAAAGAGA43010.48147103337721536No Hit
CAAGTTACACTCTAGATCCTAAACGTAAGAAGCAACACTCATTACATTAC42820.47934409786590004No Hit
AAACAAAAGTGTTGTCTTCATGCTAGTTTAAACTAATTTTTAAAAAATTA40930.45818668672702684No Hit
GTACACCACACACACACACGCACACGCACACGCAGAGCTGAGGGAAGGCT38700.4332231804626421No Hit
TCCTATATGTGATCCGCAGTTGACTGAAATGTCACGCAGCGCATGACTGT37750.42258850290606553No Hit
GTGTATTTATTGTAGCCGAGTATCTAATTAAACAAGTTTTTACTAAATCT37290.417439080089197No Hit
TAAGCAGTCACTACCATTGTATTCTATATCAACATGCTTTTATTTTGATA37090.4152002006036019No Hit
TGGGAGCTAGCAGTGTAAACAGAGTACATACATAAAAATTACATTTTAAT36620.4099388338124535No Hit
GGATTACGTTTGTGATTTTAAGCAGATTTACTTATTAGGCTCTATAACTA36340.40680440253262046No Hit
TCACTTCAGGGCACAGAGAGGTCACCCAGCCTTCTGCTTCTTCCCTCAGG35630.3988563803587581No Hit
GATACTGGTTCTACTGTTTCTAAGTATGTGATTAAAATGTACATTGTTCT35040.39225168587625264No Hit
CAGTTGTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAATCAAGTCA34440.3855350474194675No Hit
GGATTCCATCTTAAATCCATCTTTCTCTAGAACTGAGTTTACAGTATTAA34010.38072145652543815No Hit
CCTGGTCTTTGAGGGGAACAACTTATATCTTACGTTTCATCTTATGTTTC33910.3796020167826406No Hit
CCTTATAGCATAGTGGGAGACAGACACATAAACAAGAAGGAAGAAGGTGT33860.37904229691124186No Hit
AGCAGTATGTTGAGTTTATGGCAGATTAATCTATCATCTTTTAGAAATTT33180.3714301066602187No Hit
AGTTGGGTACAGTCATGGTAATGCATTATATTTTAAGATTTTGCCTTTCT33120.3707584428145402No Hit
ATCATGTGTGATTTTGTAGTTCTGTTAAAGTTCATGGCTTTTGTGTTTTA32730.3663926278176298No Hit
TGTTGGGTAGTTCCTTATGTAATGTTTTTTGTTTTTTATTAATAGGATCG32360.362250700769279No Hit
TCTTCATGCAGCTTCCCCTAATGTTAATATCTTACATACCCATGCCTGAC32240.360907373077922No Hit
GGCTACACTGGACACCTCATGGCTGTATGGCCTGGTCCAAGCCAGTGTCC28870.3231822537456454No Hit
TACCCTTATTGAGACAATGCCAACATTAATTTTAAAAACCAAAAATTCTA27970.31310729606046767No Hit
CATGGTAGTAGTATCAGTAGTAAAAGTATTTATTCCCATATGTCATTTTC27870.3119878563176701No Hit
TGCCAAACAACAAAGTGCTCAATCTACTATATGTACAAGAAGATTACCAA27370.3063906576036825No Hit
CAAACCACGCTCCTTCCACTGGGCCCAATATGACATCACAGCCCTCCCCA26900.30112929081253414No Hit
CCCCCACTTTGACAGAAGGGCAGACTGACATCCAGTCTGGGGAGATTGGG26880.30090540286397466No Hit
AGCTCTTTCTCCCTTTGCCTACTTCTCCCTCTAGTACAGAATACGCCATC25900.2899348933845589No Hit
ATCTTGGGTGCTGTGCTCAGACTCCCCCAATCTCTTCCACAGAAATCTCC25670.28736018197612456No Hit
CTAAGTAACTATCTTAAGGGTTGCTCCAAAAATCTTACCTTTTCTAGATA23620.2644116672487753No Hit
GGTGAGCACTGAGGGAATGAAAGTGGGATCAGGGATCCACTTCCTTGCCC22860.2559039252035141No Hit
CATTTTCCTGTGTCTGGCCCCCTTAGGAGGACAAAGGCCTGCCCCATGGC22260.249187286746729No Hit
CATATGATAACAGCAAATACATGTTACTTAAAATTACCATTTCTCTCATT22070.2470603512354137No Hit
CTCCCTACAGAGTCAGCCACTTAAGCTGATAGAGACATGATGTAACCATG21730.24325425610990212No Hit
CTCCCTACAGAGTCAGCCACTTAAGCTGATAGAGACATGATGTAACCGTG20760.23239569060476611No Hit
CAGTTGTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAATCAAGTCAA20520.22970903522205208No Hit
TTTCCCCAAACACAGCCTTCCTTCCCCCAGATCCCCAAGCTGCTCCATCG20350.2278059876592963No Hit
CTAAAATGCCCTCCCCTCGAACCCTTACCAAACTCCTATGCATACTACAG19930.22310434073954666No Hit
GGAGTTTCACCATATTGGCCAGGCTGTTCTCAAACTCCTGACCTTGTGAT19410.21728325407699955No Hit
GCCTCCCAAAGCATTATGAATATGGGCATGAGCCACTTCACCCAACCAAA18760.21000689574881565No Hit
CAGTAAAACACTAATCCAGCCAATAAAAAAAACAAACCGCTGCACAGCTG18690.20922328792885736No Hit
CATCTTTATTGCCCCTATATCTGTCATATTTTTATATAAAAATGTGTATA18670.20899939998029787No Hit
CAGATTTTTGAAAAGTACTACTATGTTCTCTAATAAAAAATAGTAGATGA18590.20810384818605981No Hit
TTGCTATTCTCAGATGACTCTGTGTTTTTATAATAAAATAAACTGTACTT17330.19399890742681103No Hit
TCCTTTTAGTTTGTTAATGTGATGGAATAGTTTTCAAATTATCCTTTTTT16610.18593894127866883No Hit
AAGACCAAGTCACTCTTTCTATGCAAAAACACTCACTCAGTTAACTGGTG16190.18123729435891925No Hit
TTGTTTGTTTGTTTGCTTGCTTGTTTTAAGATTGTTCCAGGACACGAAGG15950.17855063897620518No Hit
CATCACTAAAACTCTAAGGGCTAAGCCAGAGAAGGGAAGGCTCACCTTAA15770.17653564743916966No Hit
GGAAGCTTCTAATAAAATACTCATCCATATATACCTTTTTCACAAGGTGA15550.17407288000501508No Hit
CATTTCTCTTGCTTACATGAACTCTATGTCGTGGCATTCAGATCAGTCAC15220.17037872885378325No Hit
TAAGTGCAAATAGTGTATCTGACCTATTATCAATCATGTTTATACTTTTA15010.16802790539390847No Hit
GACTTCTGAATGAATTTATTTCAGAGTAATTTTCCAGAACTTACTGGTTG14990.16780401744534895No Hit
GTGTATTACCTTAATTTGAGTGATTCTTTAGATGTATTTAGTATTTGTAA14980.1676920734710692No Hit
GCCAATATTTAACCAATTTTGACCTACATAAAAATACTATTTCAAATGGT14870.16646068975399192No Hit
ACTATGAAAATGATCAGGGATATGTGAGTGTGTGTGTGTCTGTGTGTGTT14570.16310237052559934No Hit
GTGAGGGGACTTGCTAAGTATTGTTAACTAATTTTAAATTAAACAAATTT14210.15907238745152824No Hit
TTACAGTAAGTTTTGTTGGCTTACTTTAAAATTATTTCTCTCCTTATAAT14010.1568335079659332No Hit
AGTGCAGTTCAGTCTCCCAACCCCGCCTCTCCCTGCTGCCAACCAGGCAC13590.1521318610461836No Hit
GTCAAAAAATCTGGAGTTCAGTTGGGATTTTATAATTGATTGTTAAACAT12580.14082551964392861No Hit
CTGGGGCTGTCATATAGCGCTGATCCCCCCAGTTGTGCATTTACAATGGA12570.14071357566964884No Hit
TAGAGATCTCTATCTCTTAATGACATTTCTTTTTCCCTCAGGCTTTCTGT12100.13545220887850049No Hit
TTTATAAGCTTAACAGAACACATCAGTTATTTTATAACTTTAAAGTGAAC12010.1344447131099827No Hit
CACTCAAATCCTTCTAACAATACTTTATATATTTTAAATACCATTTTGAA12010.1344447131099827No Hit
TGGTGAAGTTTCATTTCATGAGGAAAAATTCACAAACAACAACCTTCAAA11810.13220583362438765No Hit
GAATGTTGTTTCTAGGTCCTACTCTAAATAATATTAACAAGCATTTAAAT10610.11877255671081738No Hit
TAAATAGTTGCCATTCCAAGTGTCTTATTTTTGTTCAAATTTATGTTTTT10020.11216786222831196No Hit
CCTCTTACCCACTCCAGGCTGTCAGCAGGGATCTTGTGAAATGTCATCTG9820.10992898274271694No Hit
CTATTTCATATTTAACCACAGTTCTTTTCCCGTAGGCTGATCCTTATTCA9760.10925731889703842No Hit
TTTGGAAGTTCACTGGTCTATGAACAAAACTTTTTAAAACGATGACTGTA9730.10892148697419915No Hit
AAAAACTCACGCGACAGTAATCTGTTAAGCCATTTATTTATAATTTATAA9710.10869759902563964No Hit
TATGTAAGACATTCTACTGCCATCTGCAGCATTCCAAATACTTCATGAAT9690.10847371107708015No Hit
TTGAGCAGCTGGGATTACAGGTGCAAAGAACCATGCCCAGCCCATGTAAT9600.10746621530856237No Hit
TTAGCTGTCAAACCTCCTAACTTCACTGTATTCTTTACTTTAGGTGTTGC9460.10589899966864584No Hit
GTCACTATTGGTAACAGAAAAGCTGCACTTTAGGATAACAAAGTCATACG9360.10477955992584831No Hit
ACTATTCCTGCTTGACCTTCAATGCTGTTCCTCAGTTTGTCACTAAAATC9320.10433178402872931No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)