FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001795600

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001795600
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences843628
Sequences flagged as poor quality0
Sequence length32-301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTCATCCTCTCCCCATAGAAAAGTCCAACCTGATCGTGGTCCAGAGA231802.7476565500433843No Hit
ATTTCCCAGGGCCTCTTACCCACTCCAGGCTGTCAGCAGGGATCTTGTGA194982.3112082576680715No Hit
ACCGGCATTGATTTTGGGACCCACATGCCCGAGGAATACACTCACAGGCT135501.6061581644990446No Hit
GAAGAACTTGAGGCTCGTGGGCGCTCAGCCTTCTCTTGTGTCTTTTTAGA131351.5569658664719521No Hit
AGATACGTTGACAACATTGGTGTGTAACAAAATCCGTATTTATAATGTGT115021.3633971371267906No Hit
TTTTCATTTTGGAAGTTCACTGGTCTATGAACAAAACTTTTTAAAACGAT114541.3577074255477533No Hit
GGAATGTTCTATTATTAAACTCATCATGTATACTTACAGGGCTTTTGGAA106801.2659608263357782No Hit
ACATAGAAAGCCAGGGTCCCAGATCAGCCACTCATTCCAGGGGAGGAAGG95421.131067247649438No Hit
AGAAGGAGAGTGTCTGTCGCTCAGTCCCACTCAGGGGCCACTCTTCTTTG94761.1232438942282617No Hit
GTCCTTTGGTGAAGCTATTTATACATGTATATCTTAGGGTTCTGTTTTTA86241.0222515137003514No Hit
AATAACTGATGTGTTCTGTTAAGCTTATAAAGTTGAACTTTTTTTTTTTT86071.020236407516109No Hit
ATGAGGCCTCTTATACTGCCAAATCAATATAAAGAAGTAAAAGAAATCCC85061.0082643060685517No Hit
AAACAAAAGTGTTGTCTTCATGCTAGTTTAAACTAATTTTTAAAAAATTA82730.9806454977786417No Hit
AAACTTGCATTCGTATCCACAGATAGCAAAAGAAAAATAGGACCACAAAA81260.9632207560678403No Hit
GAGATACACAGTCTACCTGGTAAGAAAAAAAATAAAGTCTTCCTTATAAA76370.9052568193563988No Hit
TCCTATATGTGATCCGCAGTTGACTGAAATGTCACGCAGCGCATGACTGT74970.8886618272508737No Hit
CAGTGGAGGTTAACATTCATCAAGATTAATAACTGGTGTACTTGATAGGC74870.8874764706719076No Hit
GTTTTATGTATGATCTCTTACCTATGACTCTACTGAAATAGAATTTCTAT74400.8819052947507668No Hit
ACTCTGGTTTCTGGTGGGACCATTATGGACATCAGGACATTCTCACCAAG71850.8516787019871318No Hit
CACAGACCAGGTTCCAGACATGGCTATTTTTATGCCTACAGATATCATGG70840.8397066005395742No Hit
GGTGAGCAGTATTTTAAGAAGGTCCTGTTGTCAGTTTTTCAGATTTTCTT68050.8066351519864204No Hit
CAGCACTCTTTAGATAAACAGGTCATAAACAAGGAATTATATCAGACTAA64010.7587467461961908No Hit
AAATGACTTAGTTCTGGTTGAGATGAAAGGATTCCACTGAAAGTTTTCTG63560.7534126415908434No Hit
CTCCCAAATTGCTGAGATTACAGATGTGAGCCACTGTGCCCAGCCTGATT62760.7439297889591147No Hit
CCTATTTCTCCTTCCTAACAGTTTACCAAAGTTGAATCATATATAAGCTT61330.7269791898798996No Hit
CATCAGATAAAATCCAAGAGCTTCTTCATTTAACGTAACTCACCATATGC58640.695093097905712No Hit
CACATGGCATCTGTACAGTGTCTATAACAAAATAACCCAAAGTATGAGAT56150.6655777190894565No Hit
ACAGTCATGACCCACAGCAAACAGAACTGTTTTAGATATGCTGGGTATTC56050.6643923625104904No Hit
GTGTATTTATTGTAGCCGAGTATCTAATTAAACAAGTTTTTACTAAATCT55350.6560948664577279No Hit
CAGGAGCTTCCAAATAGTATGTTCTCATTAAAAGAGGTGTTCTTGTGACA55250.6549095098787617No Hit
TTTGAGCTGTCTTGACGTTCACAGATATAAAATATTAAATATATTTTAAT54930.6511163688260703No Hit
TCTTCATGCAGCTTCCCCTAATGTTAATATCTTACATACCCATGCCTGAC54900.6507607618523804No Hit
TGGGAGCTAGCAGTGTAAACAGAGTACATACATAAAAATTACATTTTAAT54470.6456637285628263No Hit
CATATAAGGCAAAGCATTAGGTACTTGGTTTATATATTAAAGATCTTACT52640.623971703167747No Hit
CTAAAGGCTGAATGAAAGGGTAATTCATATACTGAAGATCACACCCAAGC52240.6192302768518826No Hit
AAGGTACAATGATTTCCACTTCTCTTATTTACATTTTCTAATCCCTTTCT51910.6153186001412945No Hit
TGAATGAAGAAGCAAATTCAAAACAGTAAACATTTCCTTTATACCTTTTT50200.5950490026409744No Hit
GCTCAAAAAAAAAAAAAAGAAAAAAGTGGATTTATTTTTATTTTACAGGT49910.5916114685619728No Hit
GTCAGTCAGCGTGTTTTGGTCGATCTGGGTCTCAGCACATTACTGATGGG49230.5835510438250034No Hit
CTCAAGTTTTCAGAAAAGAAGCCATGACATTAAATGTTAAAATACAGTAA48940.5801135097460018No Hit
TCCACAGCACCCAAGCTCCCCAGCCAGGCCACCCACCCCTTTGAAAGAGA48390.5735940485616884No Hit
TGTTGGGTAGTTCCTTATGTAATGTTTTTTGTTTTTTATTAATAGGATCG46530.5515464161929192No Hit
GAAGTATCATTCTCCATGAATGTCATATTGAGATTACATATAAGGTATCA46420.5502425239560566No Hit
TCACTTCAGGGCACAGAGAGGTCACCCAGCCTTCTGCTTCTTCCCTCAGG45350.5375592085611194No Hit
ATATATGGCTGTTGTGCCCTTCTCTTAGTGTTAATGAGTGCTTTTTATTT45270.5366109232979465No Hit
TTCTTTCAGCATACCACTTCATAACTGTTCAGTTTGTACAGTTTGTTCCC43460.5151559692186604No Hit
GGAAGAACAGGATAGAAAGACTGCTTATATATTGGTCTAAATATGTTATT43330.5136150056660045No Hit
CAAGTTACACTCTAGATCCTAAACGTAAGAAGCAACACTCATTACATTAC43330.5136150056660045No Hit
CAGGGAGGAAAGGAGGACAGCCAACAAGTACCAGGCAGCATGGTCAACTG43070.5105330785606926No Hit
GGAGGCTGGCCTGAGACATTCCTATGTCCTGCTCGCCCACAGGCCTTCTT43050.5102960072448994No Hit
GGATTACGTTTGTGATTTTAAGCAGATTTACTTATTAGGCTCTATAACTA42410.5027097251395165No Hit
GATACTGGTTCTACTGTTTCTAAGTATGTGATTAAAATGTACATTGTTCT41570.49275272987620133No Hit
TTCAAGCACAGGGTAGAATATTGGGCTGAGTAACACTTGCCTTCATCATG41090.48706301829716414No Hit
AAAGGTTTCAGTGAGGTGAACAGTTTAAAGGCCTTGGGAATAAGAAAATC40780.4833884129023693No Hit
AGGGGAGGAGTTCAGAGGAAGACACTTGCCAGGGAAATATCCCTGTGGCT40300.47769870132333203No Hit
CAGGAAATTTCTAAATGTGACATGACCTACTTACTGTACCTGGTGACAGA37920.44948721474393927No Hit
ATCATGTGTGATTTTGTAGTTCTGTTAAAGTTCATGGCTTTTGTGTTTTA37740.4473535729018003No Hit
AAACAGCAGCATGCTAATGAACTTAAATTACCTGTGAAGAATTGGAGGCA37660.44640528763862747No Hit
TTGAAATAGACATTGAAGGTGTCAACCAATAAACTTCTTTAAAAACTTGT36410.43158833040155137No Hit
GTTTTCTGTTGATATCTTTGATTACTTAACTTAAAAACAAAATAACTCCT36210.4292176172436192No Hit
GGATTCCATCTTAAATCCATCTTTCTCTAGAACTGAGTTTACAGTATTAA34700.4113187329012314No Hit
AGCAGTATGTTGAGTTTATGGCAGATTAATCTATCATCTTTTAGAAATTT34050.40361391513795175No Hit
CATGGTAGTAGTATCAGTAGTAAAAGTATTTATTCCCATATGTCATTTTC33690.3993466314536739No Hit
CCTTATAGCATAGTGGGAGACAGACACATAAACAAGAAGGAAGAAGGTGT33430.3962647043483621No Hit
TGCCAAACAACAAAGTGCTCAATCTACTATATGTACAAGAAGATTACCAA33210.39365691987463663No Hit
CCTCCAATAGCTTGCTTTTCACAATTGTCCTTTGTTTTGTTATAGTCCTG32830.38915256487456557No Hit
CCTGGTCTTTGAGGGGAACAACTTATATCTTACGTTTCATCTTATGTTTC32710.38773013697980624No Hit
AGCTCTTTCTCCCTTTGCCTACTTCTCCCTCTAGTACAGAATACGCCATC31810.3770619277691115No Hit
CATCTTTATTGCCCCTATATCTGTCATATTTTTATATAAAAATGTGTATA31240.37030539526900486No Hit
GGCTACACTGGACACCTCATGGCTGTATGGCCTGGTCCAAGCCAGTGTCC31040.36793468211107266No Hit
GTACACCACACACACACACGCACACGCACACGCAGAGCTGAGGGAAGGCT30420.3605854713214829No Hit
GGGTGACAGAGAAAGATCCTATCTCAAAAAACAAAACAAGAAGAAGAAAA29780.35299918921609996No Hit
GATTCTGGCCTCTCTCCCTCTCAGGGCAGGGTGCCATTTGCCGTGCCTGA29760.3527621179003068No Hit
AGTTGGGTACAGTCATGGTAATGCATTATATTTTAAGATTTTGCCTTTCT28890.34244951566330184No Hit
AGTGCAGTTCAGTCTCCCAACCCCGCCTCTCCCTGCTGCCAACCAGGCAC27690.3282252367157088No Hit
GTATGTCCAAGATCAAAGTACACTGTAAAAAGCAATACTAAACTATAATT27360.32431356000512074No Hit
GGTGAGCACTGAGGGAATGAAAGTGGGATCAGGGATCCACTTCCTTGCCC26440.31340827947863276No Hit
CATTTTCCTGTGTCTGGCCCCCTTAGGAGGACAAAGGCCTGCCCCATGGC26070.3090224601364583No Hit
CATTTCTCTTGCTTACATGAACTCTATGTCGTGGCATTCAGATCAGTCAC25030.2966947517152109No Hit
TAGAGATCTCTATCTCTTAATGACATTTCTTTTTCCCTCAGGCTTTCTGT24990.2962206090836245No Hit
CTAAAATGCCCTCCCCTCGAACCCTTACCAAACTCCTATGCATACTACAG24300.28804164868875853No Hit
TAAGTGCAAATAGTGTATCTGACCTATTATCAATCATGTTTATACTTTTA23940.2837743650044806No Hit
CTGAGAGCATCTGGTTGGGAAGGACTGATCTAACAGCGGCTGGCAGCTGG23660.2804553665833756No Hit
CAGTAAAACACTAATCCAGCCAATAAAAAAAACAAACCGCTGCACAGCTG23020.2728690844779927No Hit
ATCTTGGGTGCTGTGCTCAGACTCCCCCAATCTCTTCCACAGAAATCTCC22110.2620823396094013No Hit
ATATTTCAACAAAAAAATTATGCCTATTAGAATCAAAATATGATAGCAAA21680.2569853063198471No Hit
TTGCTATTCTCAGATGACTCTGTGTTTTTATAATAAAATAAACTGTACTT21310.2525994869776726No Hit
CATCACTAAAACTCTAAGGGCTAAGCCAGAGAAGGGAAGGCTCACCTTAA21130.25046584513553366No Hit
TATGTAAGACATTCTACTGCCATCTGCAGCATTCCAAATACTTCATGAAT20660.24489466921439307No Hit
TAAGCAGTCACTACCATTGTATTCTATATCAACATGCTTTTATTTTGATA20500.24299809868804736No Hit
CCCCCACTTTGACAGAAGGGCAGACTGACATCCAGTCTGGGGAGATTGGG20370.2414571351353914No Hit
CATTCAAGATAGAGAAAACACTGTCTGCCAAGAATAATTGTTTTTATTTC20010.23718985145111354No Hit
CAGGATATGCCACCTTTAACTCAGTTAACTGAACTTTTGTTTTTTAATAT19920.23612303053004405No Hit
TTTCCCCAAACACAGCCTTCCTTCCCCCAGATCCCCAAGCTGCTCCATCG19360.22948503368783396No Hit
CATATGATAACAGCAAATACATGTTACTTAAAATTACCATTTCTCTCATT18890.22391385776669337No Hit
TCCTTTTAGTTTGTTAATGTGATGGAATAGTTTTCAAATTATCCTTTTTT18760.22237289421403747No Hit
CTAAGTAACTATCTTAAGGGTTGCTCCAAAAATCTTACCTTTTCTAGATA18740.22213582289824424No Hit
CAAACCACGCTCCTTCCACTGGGCCCAATATGACATCACAGCCCTCCCCA18420.21834268184555278No Hit
GCCTCCCAAAGCATTATGAATATGGGCATGAGCCACTTCACCCAACCAAA17340.20554083079271906No Hit
GGAAGCTTCTAATAAAATACTCATCCATATATACCTTTTTCACAAGGTGA17070.20234036802951064No Hit
CTATTTCATATTTAACCACAGTTCTTTTCCCGTAGGCTGATCCTTATTCA16670.1975989417136463No Hit
TACCCTTATTGAGACAATGCCAACATTAATTTTAAAAACCAAAAATTCTA15420.18278198447657024No Hit
TTGTTTGTTTGTTTGCTTGCTTGTTTTAAGATTGTTCCAGGACACGAAGG15360.1820707705291906No Hit
CAGTTGTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAAATCAAGTCA15130.1793444503975686No Hit
TTAACCTGCATAAAATTTAATGTCTTCCAAACAAATGTAATAATTTTCAC14320.16974306210794332No Hit
CCTCTTCTTTATTTTCAGAGTGTCTTTTCTTTTTTGCTACTAGAGTAAAA14060.1666611350026315No Hit
CTCCCTACAGAGTCAGCCACTTAAGCTGATAGAGACATGATGTAACCATG13930.16512017144997557No Hit
CAGATTTTTGAAAAGTACTACTATGTTCTCTAATAAAAAATAGTAGATGA13920.165001635792079No Hit
TACTACCCAGCTAAAATTATCATCTTTGTAATTAAAGCTATAGCTTAAAA13770.16322360092362984No Hit
TTTATAAGCTTAACAGAACACATCAGTTATTTTATAACTTTAAAGTGAAC13620.1614455660551807No Hit
TCTTGTAGGAGTTAGGCCTTGAAAGAGAGATTTAATTGTTTTATTTGTTT13520.16026020947621464No Hit
AACAGGTAGAAATAGCCCATGTCATTTAAAATTAGCACCCTGAGAAGCTC13380.1586007102656621No Hit
GACTTCTGAATGAATTTATTTCAGAGTAATTTTCCAGAACTTACTGGTTG13090.15516317618666048No Hit
CCAATACGTGTTAAAAGCAAGTTACATTTTCTCTTTTAGGAAATTAACCA12830.15208124908134865No Hit
CTCCCTACAGAGTCAGCCACTTAAGCTGATAGAGACATGATGTAACCGTG12820.15196271342345205No Hit
CACTCAAATCCTTCTAACAATACTTTATATATTTTAAATACCATTTTGAA12480.14793250105496736No Hit
AAAAACTCACGCGACAGTAATCTGTTAAGCCATTTATTTATAATTTATAA12390.14686568013389786No Hit
ACTATTCCTGCTTGACCTTCAATGCTGTTCCTCAGTTTGTCACTAAAATC12350.14639153750231143No Hit
GTCACTATTGGTAACAGAAAAGCTGCACTTTAGGATAACAAAGTCATACG12330.14615446618651823No Hit
GTCAAAAAATCTGGAGTTCAGTTGGGATTTTATAATTGATTGTTAAACAT12240.14508764526544876No Hit
TTTGGAAGTTCACTGGTCTATGAACAAAACTTTTTAAAACGATGACTGTA12010.14236132513382674No Hit
GTATTCTTTACATGGCTTTTGGTCTTCTAAGTGAAGCTTTTTGTTTTTCT11960.1417686468443437No Hit
ACTATGAAAATGATCAGGGATATGTGAGTGTGTGTGTGTCTGTGTGTGTT11920.14129450421275727No Hit
GTGTATTACCTTAATTTGAGTGATTCTTTAGATGTATTTAGTATTTGTAA11910.14117596855486067No Hit
TTACAGTAAGTTTTGTTGGCTTACTTTAAAATTATTTCTCTCCTTATAAT11840.14034621894958443No Hit
GCCAATATTTAACCAATTTTGACCTACATAAAAATACTATTTCAAATGGT11090.13145604460733878No Hit
GTATTTCCATTTCTTAGAGGGAATGGTATATATACTTTATATAATATATA10710.12695168960726766No Hit
CATGAATGTCATATTGAGATTACATATAAGGTATCAATAAATACTCACCA10510.1245809764493355No Hit
TGGTGAAGTTTCATTTCATGAGGAAAAATTCACAAACAACAACCTTCAAA10500.12446244079143888No Hit
CCGCAGTTGACTGAAATGTCACGCAGCGCATGACTGTAGTTTACCTAGAA9800.11616494473867629No Hit
GGAGTTTCACCATATTGGCCAGGCTGTTCTCAAACTCCTGACCTTGTGAT9500.11260887500177805No Hit
TAAATAGTTGCCATTCCAAGTGTCTTATTTTTGTTCAAATTTATGTTTTT9030.10703769908063743No Hit
CAGTTGTTGTTTAGAATGAGGAGAGAGGCAAAAAAAAAAAATCAAGTCAA8890.10537819987008491No Hit
AAGACCAAGTCACTCTTTCTATGCAAAAACACTCACTCAGTTAACTGGTG8820.10454845026480865No Hit
GAATGTTGTTTCTAGGTCCTACTCTAAATAATATTAACAAGCATTTAAAT8670.10277041539635953No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)